上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (11): 1363-.

• 论著（临床研究） • 上一篇    下一篇

冻结肩患者关节囊组织中TGF-β和MMPs表达研究

李宏云1, 陈世益1, 翟伟韬2, 陈疾忤1   

  1. 1. 复旦大学 运动医学中心华山医院 运动医学与关节镜外科, 上海 200040；2. 上海市中西医结合光华医院, 上海 200052
  • 出版日期:2009-11-25 发布日期:2009-11-24
  • 通讯作者: 陈世益, 电子信箱: cshiyi@163.com。
  • 作者简介:李宏云（1978—）, 男, 住院医师, 硕士；电子信箱: lieaoon@126.com。

Expression of transforming growth factor-beta and metalloproteinases in joint capsule of frozen shoulder

LI Hong-yun1, CHEN Shi-yi1, ZHAI Wei-tao2, CHEN Ji-wu1   

  1. 1. Sports Medicine Center, Department of Sports Medicine and Arthroscopy, Huashan Hospital, Fudan University, Shanghai 200040, China|2. Shanghai Guanghua Hospital, Shanghai 200052, China
  • Online:2009-11-25 Published:2009-11-24

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摘要：

目的 研究转化生长因子-β(TGF-β)和基质金属蛋白酶（MMPs）在冻结肩发生中的可能作用。方法 以12例接受肩关节镜手术的冻结肩患者为研究对象，术中取肩袖间隙部位关节囊组织，Real-time PCR和Western blotting分别检测TFG-β、MMP1、MMP2、MMP3、MMP9、MMP12 mRNA和蛋白表达。以同期接受肩关节镜手术的12例非冻结肩患者作为对照，其中肩关节不稳2例、肩袖损伤5例、肩峰下撞击症5例。结果 冻结肩组和对照组关节囊组织TGF-β mRNA表达分别为3.36×104±2.18×103和1.85×104±3.31×103，蛋白表达分别为1.55±0.33和1.13±0.21，两组比较差异均有统计学意义（P<0.05）。冻结肩组关节囊组织MMP1、MMP2、MMP3、MMP9、MMP12 mRNA和蛋白表达均显著高于对照组（P<0.05）。结论 冻结肩的发生可能与患者关节囊组织中TFG-β和MMPs的高表达有关。

关键词: 冻结肩, 转化生长因子-β, 基质金属蛋白酶, 纤维化

Abstract:

Objective To explore the role of transforming growth factor (TFG)-β and matrix metalloproteinases（MMPs）in the development of frozen shoulder. Methods Twenty-four patients who underwent shoulder arthroscopy were included, and were divided into frozen shoulder group (n=12) and control group (n=12; n=2 for shoulder instability, n=5 for rotator cuff tear and n=5 for subacromial impingement). Joint capsule tissues at the rotator cuff interval were obtained, and the expression of TGF-β, MMP-1, MMP-2, MMP-3, MMP-9 and MMP-12 mRNA and protein was detected by Real-time PCR and Western blotting, respectively. Results The expression of TGF-β mRNA in frozen shoulder group and control group was 3.36×104±2.18×103 and 1.85×104±3.31×103, respectively, the expression of TGF-β protein was 1.55±0.33 and 1.13±0.21, respectively, and there were significant differences between these two groups （P<0.05）. The expression of MMP1, MMP2, MMP3, MMP9 and MMP12 mRNA and protein in frozen shoulder group was significantly higher than that in control group（P<0.05）. Conclusion The higher expression of TGF-β and MMPs in joint capsule of frozen shoulder may be associated with the development of frozen shoulder.

Key words: frozen shoulder, transforming growth factor-β, metalloproteinases, fibrosis