上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 200-.

• 论著（基础研究） • 上一篇    下一篇

乳腺癌高强度聚焦超声消融后残留肿瘤增殖能力的变化

牛陵川1, 邹文兵1, 张 炼2, 朱 辉2, 向理科3, 王智彪1,2   

  1. 重庆医科大学 1. 生物医学工程系超声医学工程重庆市市级重点实验室, 重庆 400016；2. 第二医院肿瘤治疗中心, 重庆 400010；3. 病理学教研室, 重庆 400016
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 作者简介:牛陵川（1977—）, 女, 博士生；电子信箱: niulingchuan@yahoo.com.cn。

Changes in proliferative capability of remnant breast tumor after ablation with high intensity focused ultrasound

NIU Ling-chuan1, ZOU Wen-bing1, ZHANG Lian2, ZHU Hui2, XIANG Li-ke3, WANG Zhi-biao1,2   

  1. 1. Department of Biomedical Engineering, Chongqing University of Medical Sciences, Chongqing Key Laboratory of Ultrasound Medical Engineering, Chongqing 400016, China；2. Clinical Center for Tumor Therapy, The Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China；3. Department of Pathology, Chongqing University of Medical Sciences, Chongqing 400016, China
  • Online:2010-02-25 Published:2010-02-25

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摘要：

目的 探讨兔乳腺种植瘤经高强度聚焦超声（HIFU）消融后残留肿瘤增殖能力的变化。方法 兔鳞癌细胞株VX2肿瘤组织块接种于新西兰兔乳腺，建立兔乳腺种植瘤模型，2周后行HIFU治疗，通过控制探针侧温度，造成肿瘤残留。56只兔子随机分为对照组(n=16)和HIFU组(n=40)，经电子显微镜和琥珀酸脱氢酶（SDH）组织化学染色检查确认消融效果和残留肿瘤组织消融后不同时间点检测增殖细胞核抗原（PCNA）的表达情况，同时观察动物生存时间和肿瘤脏器转移的情况。结果 HIFU组消融后,残留肿瘤的PCNA表达呈一过性降低，21 d后恢复至消融前水平。HIFU组的生存时间明显长于对照组，而肺部及腹腔脏器出现转移的时间明显晚于对照组（P＜0.05）。结论 即使HIFU未能一次完全消融肿瘤，在短时间内也能有效抑制残留肿瘤的生长和转移，延长机体的生存时间。

关键词: 高强度聚焦超声, 肿瘤, 消融, 增殖细胞核抗原, 乳腺癌

Abstract:

Objective To observe the changes in proliferative capability of remnant tumors implanted in rabbit breast after ablation with high intensity focused ultrasound (HIFU). Methods Rabbit implanted models were established by implanting VX2 tumor mass into the breast of New Zealand rabbits. HIFU was applied to the tumors, and the remnant tumors were harvested by controlling temperature around probe of tumors. Fifty-six rabbits were randomly divided into control group(n=10) and HIFU group(n=40). The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry at different time points after ablation, and the survival time and organ metastasis status were observed after ablation. Results After ablation in HIFU group, the positive expression of PCNA was decreased shortly in remnant tumors, and restored to the level before ablation 21 days after treatment. The survival time in HIFU group was much longer than that in control group, and the time of lung and abdominal viscera metastasis in HIFU group appeared significantly later than that in control group (P＜0.05). Conclusion Though HIFU ablation can not completely eradicate tumor after one treatment, the growth and metastasis of remnant tumors can be effectively inhibited for a short time, and HIFU ablation can prolong the survival time.

Key words: high intensity focused ultrasound, tumor, ablation, proliferating cell nuclear antigen, breast cancer