上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 404-.

• 论著（基础研究） • 上一篇    下一篇

甲状腺增生性疾病中p16INK4a基因甲基化及其表达

李晓芳1, 张 帆2, 吴平平2, 姜叙诚2   

  1. 1. 上海职工医学院病理学教研室, 上海 200237；2. 上海交通大学 基础医学院病理学教研室, 上海 200025
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 作者简介:李晓芳（1974—）, 女, 硕士；电子信箱: xingfuzhousinuo@yahoo.com.cn。
  • 基金资助:

    上海市高等学校科学技术发展基金（02BK07）

Methylation of p16INK4a gene and its expression in thyroid proliferative diseases

LI Xiao-fang1, ZHANG Fan2, WU Ping-ping2, JIANG Xu-cheng2   

  1. 1. Departtmenet of Pathology, Shanghai Medical Workers College, Shanghai200237, China；2. Departmenet of Pathology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-04-25 Published:2010-04-26
  • Supported by:

    Shanghai Colleges and Universities Science and Technology Development Fund, 02BK07

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摘要：

目的 研究甲状腺增生性疾病中p16INK4a基因的甲基化状况及其与表达之间的关系。方法 采用免疫组织化学方法检测45例甲状腺增生性疾病（15例甲状腺乳头状癌，17例甲状腺腺瘤，13例结节性甲状腺肿）样本中p16INK4a蛋白的表达；采用甲基化特异性PCR检测p16INK4a基因的甲基化状况。采用RT-PCR检测其中10例甲状腺增生性疾病（3例甲状腺乳头状癌，5例甲状腺腺瘤，2例结节性甲状腺肿）中p16INK4a mRNA 的表达。结果 45例甲状腺增生性疾病样本中，8例检测到p16INK4a基因异常甲基化，19例p16INK4a蛋白表达阳性；p16INK4A基因异常甲基化与其蛋白表达之间呈负相关(r=－0.2934，P=0.0387)。10例甲状腺增生性疾病样本中，5例检测到转录产物；p16INK4A基因异常甲基化与其转录水平之间呈负相关(r=－0.6547，P=0.04)。结论 p16INK4a基因甲基化与p16INK4a基因失活有关，但并不与失活完全一致；p16INK4a基因甲基化可能在甲状腺增生性疾病中起作用。

关键词: 甲状腺增生性疾病, p16INK4a, 甲基化

Abstract:

Objective To investigate the methylation status of p16INK4a gene and its expression in throid proliferative diseases. Methods The expression of p16INK4a protein in 45 cases of throid proliferative diseases (15 cases of papillary thyroid carcinoma, 17 cases of throid adenoma, 13 cases of nodular goiter）was detected by immunohistochemistry. Methylation status of p16INK4a gene was detected by methylation specific PCR. The expression of p16INK4a mRNA in 10 cases of thyroid proliferative diseases (3 cases of papillary thyroid carcinoma, 5 cases of throid adenoma, 2 cases of nodular goiter）was detected by RT-PCR. Results In 45 cases of thyroid proliferative diseases, aberrant methylation of p16INK4a gene was detected in 8 cases, and positive expression of p16INK4a protein was found in 19 cases. The aberrant methylation of p16INK4a gene was negatively related to its expression of protein(r=－0.2934, P=0.0387). Among the 10 cases of thyroid proliferative diseases, gene transcripts was detected in 5 cases, and the aberrant methylation of p16INK4a gene was negatively related to its transcription (r=－0.6547, P=0.04). Conclusion The methylation of p16INK4a may lead to the loss of its expression, but they are not always concordant. The methylation of p16INK4a may play a role in the pathogenesis of thyroid proliferative diseases.

Key words: thyroid proliferative disease, p16INK4a, methylation