上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 428-.

• 论著（基础研究） • 上一篇    下一篇

超声造影定量评价兔肾缺血再灌注损伤

姚洁洁1, 詹维伟2, 陈 曼2, 周建桥2, 陈 林2, 刘振华2   

  1. 1. 上海市静安区中心医院超声科, 上海 200040；2. 上海交通大学 医学院瑞金医院超声科, 上海 200025
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 作者简介:姚洁洁（1973—）, 女, 主治医师, 硕士；电子信箱: jannyfulyao@hotmail.com。

Quantitative evaluation of rabbit renal ischemia reperfusion injury by contrast-enhanced ultrasound

YAO Jie-jie1, ZHAN Wei-wei2, CHEN Man2, ZHOU Jian-qiao2, CHEN Lin2, LIU Zhen-hua2   

  1. 1. Department of Ultrasonography, Jing'an District Central Hospital, Shanghai 200040, China；2. Department of Ultrasono-graphy, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-04-25 Published:2010-04-26

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摘要：

目的 通过灰阶超声造影及其量化分析技术评价兔肾缺血后不同时间的再灌注损伤。方法 术前先对10只新西兰家兔行左肾超声造影检查作为对照（R0组，n=10）；再将10只家兔随机分为术后缺血30 min后再灌注30 min组（R1组，n=5）和再灌注60 min组（R2组，n=5），分别建立肾缺血再灌注模型。灰阶超声造影观察各组肾皮质灌注，并分析灌注峰值时间 (TP)、灌注峰值强度（A）、Qontraxt时间强度曲线（TIC）上升斜率（β）和曲线下面积（AUC）。结果 随着再灌注损伤加重，超声造影显示肾皮质灌注回声无明显变化；TP和AUC呈增加趋势，β值逐渐降低，组间差异有统计学意义（P<0.05或P<0.01），其中AUC变化显著（P<0.01）；A值无明显变化（P>0.05）。结论 AUC是评价兔肾缺血再灌注损伤的有效指标。

关键词: 肾, 缺血再灌注损伤, 灰阶超声造影, 定量分析, 家兔

Abstract:

Objective To evaluate rabbit renal ischemia reperfusion injury (IRI) by gray-scale contrast-enhanced ultrasonography (CEUS) and quantitative analysis techniques. Methods Ten New Zealand rabbits were performed left renal ultrasonography before surgery and were served as controls (R0 group, n=10), and then were randomly divided into R1 group (reperfusion for 30 min after ischemia for 30 min group, n=5) and R2 group (reperfusion for 60 min after ischemia for 30 min group, n=5) to establish the models of renal IRI. The pattern of renal perfusion was observed by CEUS, and time to peak (TP), amplitude of peak intensity(A), rising slope (β) of Qontraxt time-intensity curve and area under the curve (AUC) were analysed. Results With the increase of reperfusion injury, there was no significant change in renal cortex echo revealed by CEUS. TP and AUC increased, β values decreased (P<0.05 or P<0.01), and the change of AUC was even more significant (P<0.01). Besides, there was no significant change in A values among groups (P>0.05). Conclusion AUC is an effective index for evaluating rabbit renal IRI.

Key words: renal, ischemia reperfusion injury, gray-scale contrast-enhanced ultrasonography, quantitative analysis, rabbit