上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (7): 871-.doi: 10.3969/j.issn.1674-8115.2012.07.011

• 论著（基础研究） • 上一篇    下一篇

神经营养素3及其受体在哮喘大鼠肺组织中的表达

尹 琦, 陆晶晶, 郭忠良, 张 路, 汪 蜀, 梁永杰   

  1. 同济大学附属东方医院呼吸内科, 上海 200120
  • 出版日期:2012-07-28 发布日期:2012-08-17
  • 作者简介:尹 琦（1977—）, 女, 主治医师, 硕士；电子信箱: yvonneyinq@hotmail.com。
  • 基金资助:

    上海市卫生局青年科研项目（2009Y080）

Expression of neurotrophin-3 and its receptors in lung tissues of rats with asthma

YIN Qi, LU Jing-jing, GUO Zhong-liang, ZHANG Lu, WA NG Shu, LIANG Yong-jie   

  1. Department of Respiratory Medicine, Oriental Hospital, Tongji University, Shanghai 200120, China
  • Online:2012-07-28 Published:2012-08-17
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2009Y080

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摘要：

目的 研究神经营养素3（NT-3）及其受体酪氨酸激酶C(TrkC)和p75神经营养素受体(p75NTR)在哮喘大鼠肺组织中的表达情况。方法 将28只4~6周龄的SD大鼠随机分为分为哮喘组（n=16）（以卵蛋白致敏和激发建立大鼠哮喘模型）和正常对照组（n=12）。取两组大鼠的肺组织制作病理切片，HE染色后光学显微镜下观察支气管和肺组织的病理学特征。采用Real-Time PCR技术检测两组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的表达，并分析三者之间的相关性。结果 光学显微镜观察发现：哮喘组大鼠支气管壁周围有较多淋巴细胞和嗜酸性粒细胞浸润，可见支气管气道上皮脱落、杯状细胞增生及黏液栓增多等变化；正常对照组大鼠支气管及肺泡结构未见明显异常。哮喘组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的相对表达量均显著高于正常对照组，差异有统计学意义（P＜0.05）。相关性分析结果显示：NT-3 mRNA与TrkC mRNA的表达、NT-3 mRNA与p75NTR mRNA的表达以及TrkC mRNA与p75NTR mRNA的表达均呈正相关(r分别为0.89、0.90和0.82，均P<0.01)。结论 致敏哮喘大鼠肺组织内NT-3 mRNA表达水平升高。NT-3通过上调TrkC和p75NTR mRNA的表达，增强NT-3的功能效应，三者共同参与了哮喘气道神经源性炎症的发生。

关键词: 哮喘, 神经营养素3, 酪氨酸激酶C, p75神经营养素受体

Abstract:

Objective To determine the expression of neurotrophin-3 (NT-3) and its receptors tyrosine kinase C (TrkC) and p75 pan-neurotrophin receptor (P75NTR) in the lung tissues of rats with asthma. Methods Twenty-eight SD rats aged 4 to 6 weeks were randomized into asthma group (rat asthma model established by sensitization with ovalbumin and challenge, n=16) and normal control group (n=12). Lung tissues of rats in groups rats were obtained for pathological sections, and pathological characteristics of bronchus and lung tissues were observed with HE staining under light microscopy. The expression of NT-3, TrkC and p75NTR mRNA in the lung tissues of two groups of rats was detected by Real-Time PCR, and the correlation among them was analysed. Results Light microscopy revealed that the numbers of lymphocytes and eosinophils surrounding bronchial wall were increased, and there were bronchial airway epithelium shedding, goblet cell hyperplasia and increase of mucous suppository in asthma group, while there was no abnormal findings in the bronchial and alveolar structure in normal control group. The relative expression of NT-3, TrkC and p75NTR mRNA in lung tissues in asthma group was significantly higher than that in normal control group (P＜0.05). Correlation analysis indicated that the expression of NT-3 mRNA was positively related to that of TrkC mRNA (r=0.89, P<0.01) and p75NTR mRNA (r=0.90, P<0.01), and the expression of TrkC mRNA was positively related to that of p75NTR mRNA (r=0.82, P<0.01). Conclusion The expression of NT-3 mRNA increases in lung tissues of rats with asthma induced by sensitization. NT-3 upregulates the expression of TrkC and P75NTR mRNA, enhances the function of NT-3, which may participate in the airway neuronal inflammation in asthma.

Key words: asthma, neurotrophin-3, tyrosine kinase C, p75 neurotrophin receptor