上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (12): 1532-.doi: 10.3969/j.issn.1674-8115.2012.12.002

• 专题报道（骨关节疾病） • 上一篇    下一篇

装载趋化因子CXCL13的中空羟基磷灰石微球对间充质干细胞的趋化效应研究

易诚青1, 姚爱华2, 马春辉1, 王 谦1, 周晓凯1, 曹 云1   

  1. 1.上海交通大学附属第一人民医院骨科, 上海 200080； 2.同济大学材料科学与工程学院, 上海 201804
  • 出版日期:2012-12-28 发布日期:2012-12-31
  • 作者简介:易诚青（1974—）, 男, 副主任医师, 博士, 硕士生导师；电子信箱: ycq3000@126.com。
  • 基金资助:

    国家自然科学基金（30700853）；上海市卫生局科研基金（044Y18）

Effects of hollow hydroxyapatite microspheres loaded with chemokine CXCL13 on migration of mesenchymal stem cells

YI Cheng-qing1, YAO Ai-hua2, MA Chun-hui1, WANG Qian1, ZHOU Xiao-kai1, CAO Yun1   

  1. 1.Department of Orthopaedics, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China；2.Institute of Materials Science and Engineering, Tongji University, Shanghai 201804, China
  • Online:2012-12-28 Published:2012-12-31
  • Supported by:

    National Natural Science Foundation of China, 30700853；Shanghai Municipal Health Bureau Foundation, 044Y18

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摘要：

目的 以中空羟基磷灰石（HA）微球装载趋化因子CXCL13，观察其对间充质干细胞（MSCs）的趋化效应。方法 采用压力渗透法将重组人CXCL13 (rhCXCL13)装载于中空HA微球，ELISA法测定其体外释放曲线。以含CXCL13浸提液处理的MSCs为实验组，单纯DMEM处理的MSCs为对照组，通过Boyden小室法与活细胞工作站观察两组MSCs的迁移率、迁移速率和迁移效率，流式细胞术测定两组MSCs中CXCL13特异性受体CXCR5的表达。结果 rhCXCL13最终累积释放率约为80%，释放周期延续至30 d以上。实验组MSCs的迁移率、迁移速率和迁移效率均高于对照组，且实验组MSCs中CXCR5表达增强。结论 中空HA微球是CXCL13的有效控释载体，其释放的CXCL13具有对MSCs良好的趋化效应，可能与CXCR5受体上调有关。

关键词: 趋化因子, 间充质干细胞, 羟基磷灰石

Abstract:

Objective To investigate the effects of hollow hydroxyapatite (HA) microspheres loaded with chemokine CXCL13 on the migration of mesenchymal stem cells (MSCs). Methods Recombinant human CXCL13 (rhCXCL13) was loaded in the HA microspheres, and the in vitro release behavior of rhCXCL13 was observed. MSCs treated with CXCL13 were served as experiment group, and those treated with DMEM were used as control group. Modified Boyden's chamber method and cell station were employed to determine the migration rate, migration velocity and migration efficiency of MSCs in two groups. Furthermore, the expression of CXCR5, the specific receptor of CXCL13, was detected in MSCs by flow cytometry. Results It was found that rhCXCL13 could be gradually released from the hollow HA microspheres for an extended period of time up to 30 d, with a cumulative release rate of 80%. The migration rate, migration velocity and migration efficiency of MSCs in experiment group were significantly higher than those in control group. In addition, the expression of CXCR5 in MSCs increased significantly in experiment group. Conclusion The delivery system of hollow HA microspheres is an effective controlled release scaffold for CXCL13, and the released CXCL13 has a favorable chemotaxis to MSCs, which may be related to the up-regulation of CXCR5.

Key words: chemokine, mesenchymal stem cells, hydroxyapatite