上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (4): 409-.doi: 10.3969/j.issn.1674-8115.2013.04.006

• 论著（基础研究） • 上一篇    下一篇

硝化花粉对哮喘小鼠气道炎症的影响

杨 玲, 韩 丽, 王根发, 田 烨, 周 妍   

  1. 上海交通大学 医学院附属新华医院老年科, 上海 200092
  • 出版日期:2013-04-28 发布日期:2013-05-03
  • 通讯作者: 王根发, 电子信箱: genfa_wang@163.com。
  • 作者简介:杨 玲（1973—）, 女, 副主任医师, 博士； 电子信箱: yanglingjoy@hotmail.com。
  • 基金资助:

    国家自然科学基金（81200017）；上海市卫生局基金（20114310）；上海交通大学医工合作课题（YG2010MS12）

Effect of nitrated pollen on airway inflammation in asthmatic mice

YANG Ling, HAN Li, WANG Gen-fa, TIAN Ye, ZHOU Yan   

  1. Department of Geriatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2013-04-28 Published:2013-05-03
  • Supported by:

    National Natural Science Foundation of China, 81200017； Shanghai Municipal Health Bureau Foundation, 20114310；Foundation of Shanghai Jiaotong University, YG2010MS12

PDF (PC)

1408

摘要：

目的 观察硝化花粉诱导的哮喘小鼠气道的过敏性炎症及肺部的病理变化。方法 60只小鼠按免疫及激发物的不同分为5组，其中A~D组为哮喘模型组，E组为正常对照组。取小鼠的右肺组织，HE染色后观察肺组织病理学变化，计算气管壁厚度（WAt/Pbm）和气道平滑肌厚度（WAm/Pbm）；观察支气管肺泡灌洗液（BALF）的细胞学改变；免疫组织化学法检测肺组织内核因子-κB （NF-κB）p65活化和三硝化酪氨酸（3-NT）的表达；TUNEL法检测肺组织内嗜酸性粒细胞（EOS）的凋亡情况。结果 各哮喘模型组小鼠BALF中白细胞总数、EOS和中性粒细胞（NEU）分类计数及其占总细胞数的百分比以及WAt/Pbm和WAm/Pbm、肺组织NFκB p65核表达阳性率、3-NT阳性信号均显著高于E组，差异有统计学意义（P<0.01）；但各模型组小鼠肺组织的EOS凋亡率显著低于E组，差异有统计学意义（P<0.01）；用尾气硝化花粉免疫和激发的D组较其他模型组的变化更显著（P<0.05或P<0.01）。Pearson相关分析显示：3-NT表达量与EOS凋亡率呈显著负相关（r=-0.632，P<0.05）；与NF-κB p65核表达阳性率呈显著正相关（r=0.667，P<0.05）。结论 尾气硝化花粉诱发的哮喘小鼠肺组织氧化应激水平升高，使3-NT表达增多，从而激活NF-κB信号通路，使EOS凋亡延迟，产生更严重的气道炎症和病理改变。

关键词: 空气污染, 花粉, 哮喘, 氧化应激, 炎症

Abstract:

Objective To observe the allergic airway inflammation and lung pathology in mice with asthma induced by nitrated pollen. Methods Sixty mice were randomly divided into 5 groups according to different sensitization and challenge. Group A to group D were asthma model groups, and group E was normal control group. The right lung tissues of mice were obtained, the pathological changes of lung tissues were observed with HE staining, and the tracheal wall thickness (total wall area/basement membrane perimeter, WAt/Pbm) and airway smooth muscle thickness (smooth muscle area/basement membrane perimeter, WAm/Pbm) were calculated. The cytological changes in bronchoalveolar lavage fluid (BALF) were observed, the level of nuclear factor kappa B (NF-κB) p65 activation and expression of 3-nitrotyrosine (3-NT) in lung tissues were determined by immunohistochemical method, and the apoptosis of eosinophils (EOS) in lung tissues was detected by TUNEL. Results The numbers of white cells, EOS and neutrophil (NEU) and percents of them in total cells in BALF, WAt/Pbm and WAm/Pbm, the level of NF-κB p65 activation and expression of 3-NT in each asthma model group were significantly higher than those in group E (P<0.01), while the apoptosis rate of eosinophils in lung tissues in each group was significantly lower than that in group E (P<0.01). Compared with the other model groups, the values of those indicators in group D in which mice were immunized and challenged with nitrated pollen changed more significantly (P<0.05 or P<0.01). Pearson correlation analysis revealed that the expression of 3-NT was significantly negatively correlated with apoptosis rate of EOS (r=－0.632, P<0.05), and was significantly positively correlated with the level of NF-κB p65 activation (r=0.667, P<0.05). Conclusion High level of oxidative stress which exists in asthmatic mice immunized and challenged with nitrated pollen may elevate the expression of 3-NT, activate NF-κB signaling pathway, delay apoptosis of EOS, and result in more severe airway inflammation and pathological changes.

Key words: air pollution, pollen, asthma, oxidative stress, inflammation