›› 2019, Vol. 39 ›› Issue (7): 730-.doi: 10.3969/j.issn.1674-8115.2019.07.007

• Original article (Basic research) • Previous Articles     Next Articles

Improved efficiency of adeno-associated virus mediated gene transfer to vein graftsof pluronic F-127-trypsin gel

LI Song1, ZHONG Chang-ming1, WANG Xiao-wen1, 2, ZHANG Cheng1, 3, FENG Bo2, XIE Xiao4, HUANG Chun1, XIANG Xiao-yong1   

  1. 1. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2. Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong 999077, China; 3. Centre for Clinical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London EC1M 6BQ, United Kingdom; 4. Department of Cardiovascular Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2019-07-28 Published:2019-08-26
  • Supported by:
    National Natural Science Foundation of China, 81700320; Chongqing Science and Technology Commission Basic Research Funding, CSTC 2015JCYA10099

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Abstract: Objective · To investigate whether pluronic F-127-trypsin gel enhances the efficiency of adeno-associated virus (AAV) mediated gene transfer to vein grafts. Methods · Rat model of venous bridge vascular restenosis was established. The vein grafts were infected with recombinant AAV encoding an enhanced green fluorescent protein (Egfp) reporter gene, and pluronic F-127-trypsin gel was used to increase viral contact time and viral penetration. The of EGFP in vein grafts was determinedfrozen section, immunohistochemistry staining and quantitative reverse transcriptase-mediated PCR 21 days after surgery. Structural integrity of the tissue was evaluatedmeasurement of tissue tensile strength. Results · Pluronic F-127-trypsin gel can significantly improve the efficiency of AAV infection in vein grafts, in which AAV serotype 6 was more efficient than AAV 1, 8, and 9 serotypes in infecting vein grafts, and tissue tensile strength was not affected. Conclusion · Pluronic F-127 trypsin gel may be a safe and effective method to improve the efficiency of AAV mediated gene transfer to vein grafts. It can be used for the prevention and treatment of venous bridge vascular restenosis.

Key words: gene therapy, adeno-associated virus, pluronic F-127, vein grafts, restenosis

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