›› 2019, Vol. 39 ›› Issue (7): 721-.doi: 10.3969/j.issn.1674-8115.2019.07.006

• Original article (Basic research) • Previous Articles     Next Articles

Effects of BRD4 inhibitor on histone crotonylation, proliferation and migration of prostate cancer cells

HONG Xi1, LIU Li-jie1, HUANG Xian-yu2, LUO Jing2, YU Jian-jun1   

  1. 1. Department of Urology, Shanghai Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China; 2. Academy of Life Sciences, East China Normal University, Shanghai 200241, China
  • Online:2019-07-28 Published:2019-08-26
  • Supported by:
    “the Belt and Road” Project of Shanghai Science and Technology Commission, 17410742300

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Abstract: Objective · To investigate the effects of bromodomain-containing protein 4 (BRD4) inhibitor I-BET762 on histone crotonylation, proliferation and migration of prostate cancer cells. Methods · Three human prostate cancer cell lines, i.e., LNCaP, C4-2B and PC-3, were respectively treated with I-BET762 of half maximal inhibitory concentration. Histone crotonylation modification and acetylase were detectedWestern blotting. CCK-8, transwell migration test and scratch test were used to detect the effects of I-BET762 on proliferation and migration of LNCaP, C4-2B and PC-3 cells. Results · I-BET762 inhibited the of histone acetylase P300 and GCN5, and reduced the histone crotonylation modification. Transwell migration test and scratch test showed that I-BET762 could inhibit the migration of prostate cancer cell lines LNCaP, C4-2B and PC-3 (all P<0.01); CCK-8 test showed that the proliferation of three prostate cancer cell lines was inhibitedI-BET762. Conclusion · In prostate cancer cells, I-BET762 can reduce the histone crotonylation and also inhibit cell proliferation and migration.

Key words: histone crotonylation, prostate cancer, bromodomain-containing protein 4 (BRD4), inhibitor, proliferation, migration

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