Journal of Shanghai Jiao Tong University (Medical Science) ›› 2023, Vol. 43 ›› Issue (2): 143-151.doi: 10.3969/j.issn.1674-8115.2023.02.002

• Basic research • Previous Articles    

Quantitative characteristics of serum lipoprotein phenotypes for HBV patients

YI Xiaoxuan1(), HU Senlin2, SUN Yang2, HUANG Qingxia1(), TANG Huiru1,3()   

  1. 1.School of Life Sciences, Human Phenome Institute, State Key Laboratory of Genetic Engineering, Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics, Shanghai 200438, China
    2.Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    3.Zhongshan Hospital, Fudan University, Shanghai 200032, China
  • Received:2022-12-08 Accepted:2023-02-03 Online:2023-02-28 Published:2023-02-28
  • Contact: HUANG Qingxia,TANG Huiru;;
  • Supported by:
    National Key R&D Program of China(2021YFC2500600);National Natural Science Foundation of China(31821002)


Objective ·Previous studies showed that hepatitis B virus (HBV) infection caused outstanding changes in host lipoproteins. However, there are no reports on such component changes of lipoprotein subfractions. This study aimed to quantify the HBV-caused changes in the serum lipoprotein subfractions and their components. Methods ·Hepatitis B surface antigen-positive [HBsAg (+)] patients at Division of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from March to June 2017 were included (n=40), and 40 HBsAg-negative [HBsAg (-)] population were matched as controls. Serum lipoprotein subfractions and their components were quantified by using 1H-NMR. Orthogonal partial least-squares discriminant analysis (OPLS-DA), variance analysis between the two groups, logistic regression analysis and Spearman correlation analysis were conducted to reveal the lipoprotein changes in chronic HBV patients against controls. Results ·HBsAg (+) population had significantly lower levels in most lipoprotein subfractions than HBsAg (-) population. After adjustments for age, gender, hypertension, diabetes mellitus and coronary heart disease, the levels of total VLDL, VLDL1-VLDL3, IDL, HDL4, non-HDL and their components were protective factors for HBV infection (OR < 1, P < 0.01). In contrast, VLDL5-(TAG/LP) was a risk factor for HBV infection (OR > 1, P < 0.01). In addition, the severity of inflammation in the HBsAg (+) population was negatively correlated with the levels of lipids in HDL4 with correlation coefficient ranging from -0.71 to -0.51(P≤ 0.002). Six lipoprotein subfractions were obtained through feature screening, and the AUC of HBV infection diagnosis model was 0.861. Conclusion ·HBV infection causes significant changes in liver-excretion of lipoproteins and their circulation metabolism; the lipoprotein phenotypes can differentiate HBV-infected patients from controls.

Key words: lipoprotein, 1H-NMR, hepatitis B virus, chronic hepatitis B

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