›› 2011, Vol. 31 ›› Issue (10): 1398-.doi: 10.3969/j.issn.1674-8115.2011.10.009

• Original article (Basic research) • Previous Articles     Next Articles

Establishment of mouse model of cyclophosphamide-induced ovarian failure

WANG Fang-yuan1, XIE Chao2, CHENG Wei-wei1, HAN Wei3, LAI Dong-mei1   

  1. 1.Central Research Laboratory, the International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China；2.Shanghai Municipal Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China；3.Laboratory of Regeneration Medicine, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2011-10-28 Published:2011-10-27
  • Supported by:

    Foundation of the International Peace Maternity and Child Health Hospital affiliated to Shanghai Jiaotong University School of Medicine, 3030448；Shanghai Jiaotong University Foundation, YG2010MS66

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Abstract:

Objective To establish the mouse model of chemotherapy-induced ovarian failure. Methods Female C57BL/6 mice were intraperitoneally injected with cyclophosphamide at the dose of 75 mg/kg. The general conditions, estrous cycles, serum estrogen (estradiol) levels, morphology of ovarian tissues, changes of numbers of follicles, secretion of anti-Mullerian hormone and tissue fibrosis of mice were observed. Mice without cyclophosphamide injection were served as controls. Results Compared with controls, the serum estrogen level and secretion of anti-Mullerian hormone of ovarian tissues decreased, there were significant stromal hyperplasia and fibrosis in ovarian tissues, and the number of follicles reduced in mice injected with cyclophosphamide. Conclusion Intraperitoneal injection of 75 mg/kg cyclophosphamide may cause obvious ovarian failure, and the changes in reproductive endocrine and ovarian histopathology are similar to those of chemotherapy-induced ovarian failure in female patients in clinics. It can be used to establish the animal model of chemotherapy-induced ovarian failure due to its simplicity, feasibility, high success rate and low mortality.

Key words: ovarian failure, chemotherapy, animal model, mouse