›› 2011, Vol. 31 ›› Issue (10): 1393-.doi: 10.3969/j.issn.1674-8115.2011.10.008

• Original article (Basic research) • Previous Articles     Next Articles

Effects of androgen and high-fat diet on ultrastructures of ovary, pituitary and adrenal in female rats

ZHAI Hua-ling1, WU Hui1, ZHANG Lan2, LU Ying-li1   

  1. 1.Department of Endocrinology and Metabolism, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai200011, China；2.Department of Biochemical and Cell Molecular Biology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-10-28 Published:2011-10-27
  • Supported by:

    Foundation of Shanghai Jiaotong University School of Medicine, YZ1001

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Abstract:

Objective To investigate the effects of androgen and high-fat diet on ultrastructures of ovary, pituitary and adrenal in female SD rats. Methods Twenty-four female SD rats aged 21 d were randomly divided into control group, androgen injection group and androgen injection+high-fat diet group. Eight weeks after treatment, serum concentrations of glucose, insulin, testosterone, triglyceride and cholesterol were measured, and ultrastructures of ovary, pituitary and adrenal were observed by transmission electron microscopy. Results The serum concentrations of glucose, insulin, testosterone, triglyceride and cholesterol in androgen injection group and androgen injection+high-fat diet group were higher than those in control group. Polycystic changes of ovaries and increasing apoptosis in pituitaries and adrenals were observed in androgen injection group and androgen injection+high-fat diet group, and increasing lipids were found in androgen injection+high-fat diet group. Conclusion Androgen can induce the polycystic ovaries and increase the blood glucose in female rats. Androgen combined with high-fat diet may well meet the modeling requirements for polycystic ovary syndrome in female rats.

Key words: polycystic ovary syndrome, ultrastructure, androgen, high-fat diet