›› 2018, Vol. 38 ›› Issue (2): 151-.doi: 10.3969/j.issn.1674-8115.2018.02.007

• Original article (Basic research) • Previous Articles     Next Articles

Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation

PAN Xu-feng1*, LI Jian2*, PAN Yan3, GU Chang1, GUO Zhen2, YANG Jun1   

  1. 1. Department of Thoracic Surgery, 2. Department of Extracorporeal Circulation, 3. Department of Pharmacy, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    Shanghai Municipal Commission of Health and Family Planning Project for Young Scientists, 20144Y0169

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Abstract: Objective · To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold
storage group. Methods · Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP. The biological
index, wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups. Results · Wet-to-dry weight ratio of lung tissue in
EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48, P=0.012); Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg
vs (300.5±21.6) mmHg, P=0.023]; Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL,
P=0.043]. Conclusion · EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.

Key words: ex vivo lung perfusion, cold storage, lung transplantation, rat