Abstract: Objective · To investigate the effect of poly (L-lactic acid caprolactone) (PLCL) /gelatin electrospinning on the angiogenesis differentiation of endothelial progenitor cells (EPCs). Methods · Rat bone marrow-derived EPCs were isolated and cultured, then identification was performed. After preparation of PLCL/gelatin blend electrospun scaffold, scanning electron microscopy and water contact angle test were carried out. EPCs were grown on PLCL/gelatin electrospinning and CCK8 was used to detect cell proliferation. The of vascular endothelial growth factor (Vegf ) and kinases region receptor (Kdr) was observedRT-PCR and the of VEGF protein was observedWestern blotting. Results · The density gradient centrifugation combined with differential adherence method could effectively isolate EPCs. PLCL/gelatin electrospun nanofibers were porous, and the hydrophilic properties were favorable for cell adhesion, and EPCs grew well on the scaffold. The of Vegf and Kdr gene in PLCL/gelatin group was higher than that in control group (P0.000), and the of VEGF protein was also increased (P0.000). Conclusion · PLCL/gelatin is an ideal scaffold for tissue engineering, and it can promote the angiogenesis differentiation of EPCs.

Key words: poly (L-lactic acid caprolactone), endothelial progenitor cell, isolation and culture, biological identification, electrospun, angiogenesis