›› 2019, Vol. 39 ›› Issue (12): 1394-.doi: 10.3969/j.issn.1674-8115.2019.12.009

• Original article (Basic research) • Previous Articles     Next Articles

Effect of miR-218-2-3P on proliferation and apoptosis of NK/T-cell lymphomatargeting SIN3A

WANG Jia-lin, JI Di, CHEN Xiang, YANG Bo, YU Lin   

  1. Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2019-12-28 Published:2020-02-06
  • Supported by:
    Science and Technology Benefit Project of Chongqing Science and Technology Commission, cstc2015jcsf10001-02-03

PDF (PC)

377

Abstract: Objective · To investigate the of miR-218-2-3P in NK/T-cell lymphoma, and the effect of miR-218-2-3P on the proliferation, apoptosis and cycle of NK/T-cell lymphomatargeting SIN3A. Methods · Quantitative real-time PCR (qPCR) was used to detect the s of miR-218-2-3P in normal NK cells and NK/T-cell lymphoma cells NK92MI and NKYS. Lipofectamine 3000 was used to transfect the inhibitor containing nonsense sequences (inhibitors NC), miR-218-2-3P inhibitor and the same dose of transfection reagent without any fragment into NK92MI cells, which were divided into three groups. qPCR and Western blotting were used to detect the levels of miR-218-2-3P and SIN3A protein in the inhibitor NC group, the miR-218-2-3P inhibitor group and the blank control group, respectively. The cell proliferation activities of the three group were measuredCCK8 method. The apoptosis rates and cell cycles of the three group were determinedflow cytometry. The double luciferase reporter gene assay was performed to detect whether SIN3A was a target gene of miR-218-2-3P. NK92MI cells were transfected with miR-218-2-3P inhibitor+SIN3A small interfering RNA (si-SIN3A) and miR-218-2-3P inhibitor+nonsense sequences small interfering RNA (si-NC), respectively, which were divided into two groups. The cell proliferation activities of the two groups were detectedCCK8 method. Results · Compared with the normal NK cells, the s of miR-218-2-3P in NK92MI and NKYS cells significantly increased (both PPPPSIN3A was the target gene of miR-218-2-3P. Compared with the blank control group and the inhibitor NC group, the SIN3A protein of the miR-218-2-3P inhibitor group was increased (both PSIN3A could restore the proliferation activity of cells weakenedmiR-218-2-3P inhibitor (both PConclusion · miR-218-2-3P is highly expressed in the NK92MI and NKYS cell lines. miR-218-2-3P may affect the proliferation, apoptosis and cycle of NK/T-cell lymphoma through targeted regulation of SIN3A.

Key words: NK/T-cell lymphoma, miR-218-2-3P, SIN3A, proliferation, apoptosis, cell cycle