›› 2019, Vol. 39 ›› Issue (7): 706-.doi: 10.3969/j.issn.1674-8115.2019.07.004

• Original article (Basic research) • Previous Articles     Next Articles

SUMO specific peptidase 3 regulates autophagy in motesticular Sertoli cells

TAO Ya-qun1, ZHU Hui-qin1, PAN Yi-qing2, LAO Yi-min1, YI Jing1, YANG Jie1   

  1. 1. Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China; 2. Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
  • Online:2019-07-28 Published:2019-08-26
  • Supported by:
    National Natural Science Foundation of China, 31771522; Natural Science Foundation of Shanghai, 16ZR1418400; Shanghai Charity Race for Cancer Research Fund 2016, SCCRC16004

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Abstract: Objective · To investigate the regulation of autophagySUMO specific peptidase 3 (SENP3, normally called SUMO specific protease 3) in motestis. Methods · Immunofluorescence was used to detect the localization of SENP3 in spermatogenic cells and Sertoli cells of testis. Senp3 wild type (Senp3+/+) mice and Senp3 gene knockout heterozygous (Senp3+/-) mice were subjected to starvation treatment to induce autophagy. Testicular tissue proteins were extracted, and the extent of autophagy was detectedWestern blotting. The extent of autophagy of Sertoli cells was detected and compared with that of spermatogenic cells in testistransmission electron microscopy and immunofluorescence. Results · SENP3 mainly localized in the nucleus of Sertoli cells. Compared to Senp3+/+ mice, the extent of starvation-induced autophagy in Sertoli cells of Senp3+/- mice increased. Conclusion · SENP3 can inhibit autophagy in Sertoli cells during nutrient deficiency, which may play a role in controlling the extent of autophagy.

Key words: autophagy, SUMO specific protease 3, testis, Sertoli cell

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