JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2022, Vol. 42 ›› Issue (1): 36-43.doi: 10.3969/j.issn.1674-8115.2022.01.006
• Basic research • Previous Articles Next Articles
Yanna LIU1(), Zhaorui REN1,2, Jingbin YAN1,2(
)
Received:
2021-09-27
Online:
2022-01-28
Published:
2022-02-18
Contact:
Jingbin YAN
E-mail:www.liuyanna@163.com;m18917128323@163.com
Supported by:
CLC Number:
Yanna LIU, Zhaorui REN, Jingbin YAN. Enhancement of BMP4 inhibitor DMH1 on the efficiency of BiSF in human iPSC-induced differentiation into neurons[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2022, 42(1): 36-43.
Primer name | Forward (5'→3') | Reverse (5'→3') |
---|---|---|
GAPDH | TTCCAAGCATAAAAGAACGG | TACATCCTCATCACCACCCA |
NANOG | TTTGTGGGGCTGAAGAAACT | AGGGCTGTCCTGAATAAGCAG |
OCT4 | GACGCCATCAACACCGAGTT | CTTTGTCGTTGGTTAGCTGGT |
Nestin | CAGCGTTGGAACAGAGGTTGG | TGGCACAGGTGTCTCAAGGGTAG |
PAX6 | ATGTGTGAGTAAAATTCTGGGCA | GCTTACAACTTCTGGAGTCGCTA |
MAP2 | CCACCTGAGATTAAGGATCA | GGCTTACTTTGCTTCTC |
Tab 1 Genes and primer sequences used for qRT-PCR analysis
Primer name | Forward (5'→3') | Reverse (5'→3') |
---|---|---|
GAPDH | TTCCAAGCATAAAAGAACGG | TACATCCTCATCACCACCCA |
NANOG | TTTGTGGGGCTGAAGAAACT | AGGGCTGTCCTGAATAAGCAG |
OCT4 | GACGCCATCAACACCGAGTT | CTTTGTCGTTGGTTAGCTGGT |
Nestin | CAGCGTTGGAACAGAGGTTGG | TGGCACAGGTGTCTCAAGGGTAG |
PAX6 | ATGTGTGAGTAAAATTCTGGGCA | GCTTACAACTTCTGGAGTCGCTA |
MAP2 | CCACCTGAGATTAAGGATCA | GGCTTACTTTGCTTCTC |
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