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Effects of N-stearoyltyrosine on H2O2-induced oxidative-stress damage of PC12 cells

YAO Li-yun, LIN Qi, LU Yang   

  1. Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2014-05-28 Published:2014-05-30
  • Supported by:

    Natural Science Foundation of Shanghai Science and Technology Commission Municipality, 11ZR1419200; National Natural Science Foundation of China, 81171245; Key Basic Projects of Shanghai Science and Technology Commission Municipality, 11JC1406600

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Abstract:

Objective To investigate the effects of N-stearoyltyrosine (NsTyr) pretreatment on oxidative stress damage of PC12 cells induced by H2O2. Methods PC12 cells cultured in vitro were divided into the blank control group, H2O2 damage group (induced by H2O2 of different concentrations for 16 h), and NsTyr pretreatment group (induced by H2O2 of 100 μmol/L for 16 h after being pretreated by NsTy of different concentrations for 30 min). The apoptosis rate and ROS level were detected by the flow cytometer. The changes of expressions of Bax and Bcl-2 were determinated by the Western blotting. Results Compared to the H2O2 (100 μmol/L) damage group, the survival rates of PC12 cells of the NsTyr (5 and 10 μmol/L) pretreatment groups were significantly increased (P<0.01); the apoptotic rates of PC12 cells decreased (P<0.05); the intracellular production of ROS decreased (P<0.05, P<0.01); and the ratios of Bcl-2/Bax increased (P<0.05). Conclusion NsTyr can protect PC12 cells against H2O2-induced oxidative-stress damage; up-regulate the expression of Bcl-2; down-regulate the expression of Bax; and inhibit the apoptosis.

Key words: N-stearoyltyrosine, H2O2, PC12 cells, oxidative stress