›› 2018, Vol. 38 ›› Issue (5): 499-.doi: 10.3969/j.issn.1674-8115.2018.05.003

• Original article (Basic research) • Previous Articles     Next Articles

Effect of poly (L-lactic acid caprolactone)/gelatin blend electrospun on angiogenesis of rat bone marrow-derived endothelial progenitor cells

ZHANG Xia, ZHENG Lei-lei, LIU Yan, MING Ye, HE Hao-jue, HU Yun   

  1. Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 401147, China
  • Online:2018-05-28 Published:2018-05-28
  • Supported by:
    National Natural Science Foundation of China, 81470772; Chongqing Medical Research Project, 20141013, 2015HBRC009; Chongqing Natural Science Foundation, cstc2015jcyj10028, cstc2016jcyjA0238

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Abstract: Objective · To investigate the effect of poly (L-lactic acid caprolactone) (PLCL) /gelatin electrospinning on the angiogenesis differentiation of endothelial progenitor cells (EPCs). Methods · Rat bone marrow-derived EPCs were isolated and cultured, then identification was performed. After preparation of PLCL/gelatin blend electrospun scaffold, scanning electron microscopy and water contact angle test were carried out. EPCs were grown on PLCL/gelatin electrospinning and CCK8 was used to detect cell proliferation. The of vascular endothelial growth factor (Vegf ) and kinases region receptor (Kdr) was observedRT-PCR and the of VEGF protein was observedWestern blotting. Results · The density gradient centrifugation combined with differential adherence method could effectively isolate EPCs. PLCL/gelatin electrospun nanofibers were porous, and the hydrophilic properties were favorable for cell adhesion, and EPCs grew well on the scaffold. The of Vegf and Kdr gene in PLCL/gelatin group was higher than that in control group (P0.000), and the of VEGF protein was also increased (P0.000). Conclusion · PLCL/gelatin is an ideal scaffold for tissue engineering, and it can promote the angiogenesis differentiation of EPCs.

Key words: poly (L-lactic acid caprolactone), endothelial progenitor cell, isolation and culture, biological identification, electrospun, angiogenesis

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