Abstract:

Objective To investigate the gene expression pattern of metabotropic glutamate receptor-Ⅰ(mGluR-Ⅰ), D-p-hydroxyphenylglycine (DHPG)-induced rat hippocampal slice epileptic seizure model. Methods In vitro rat hippocampal sclice was continously perfused with artificial cerebrospinal fluid containing 50 μmol DHPG, and epileptic seizure model was established (DHPG group, n=3). cDNA microarray chip was applied to explore the gene expression pattern in DHPG group, the differentially expressed genes were screened in comparison with control group (n=3), and functional classification analysis was conducted. Results There were 206 up-regulated genes and 489 down-regulated genes, among which 67 up-regulated genes and 86 down-regulated genes differentially expressed by 1.5 fold, 6 up-regulated genes differentially expressed by more than 2.0 folds, and 25 down-regulated genes differentially expressed by less than 0.5 fold. Functional classification analysis revealed that differentially expressed gene function involved in protein binding (19 genes), molecular function, calciumion binding and nucleotide binding. Conclusion Epileptic seizure and roles of mGluR-Ⅰagonist may be related to various genes, which is a complicated process. This experiment provides evidences for further researches.

Key words: metabotropic glutamate receptor, D-p-hydroxyphenylglycine, microarray, gene expression, hippocampal sclice, epilepsy