›› 2011, Vol. 31 ›› Issue (7): 971-.doi: 10.3969/j.issn.1674-8115.2011.07.022

• Original article (Clinical research) • Previous Articles     Next Articles

Detection of mass concentrations of ATP in CD4+T cells for immunity monitoring in renal transplant recipients

LI Chun-jing, ZHOU Pei-jun, XU Da, WANG Xiang-hui, SHAO Kun   

  1. Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 09411952600

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Abstract:

Objective To investigate the value of detection of mass concentrations of adenosine triphosphate (ATP) in CD4+ T cells in immunity monitoring of renal transplant recipients. Methods Seventy renal transplant recipients were divided into infection group (with infection, n=20) and control group (without infection, n=50) according to the clinical status, and both groups received immunosuppressive therapy based on calcineurin inhibitors of ciclosporin and tacrolimus. Peripheral blood samples were collected from renal transplant recipients. The mass concentrations of ATP in CD4+ T cells in peripheral blood were detected by ImmuKnow method, the percentages of T lymphocyte subset of CD3+ T cells, CD4+ T cells and CD8+ T cells and ratio of CD4+ T cells to CD8+ T cells (CD4+/CD8+) in peripheral blood were determined by flow cytometry, and the plasma mass concentrations of ciclosporin and tacrolimus were measured by homogeneous immunoassay. The correlations of mass concentrations of ATP in CD4+ T cells in peripheral blood with the other parameters were analysed. Results The mass concentration of ATP in CD4+ T cells in peripheral blood in infection group was significantly lower than that in control group (P<0.05), and the percentages of CD3+ T cells and CD8+ T cells in infection group were significantly higher than those in control group (P<0.05). There was no significant difference in percentage of CD4+ T cells and CD4+/CD8+ in peripheral blood, and plasma concentrations of ciclosporin and tacrolimus between two groups (P>0.05). Correlation analysis revealed that the mass concentration of ATP in CD4+ T cells in peripheral blood was significantly negatively related to the percentage of CD8+ T cells in peripheral blood (r=－0.299, P=0.041), and was significantly positively related to CD4+/CD8+ (r=0.324, P=0.026). Conclusion Compared with detection of T lymphocyte subset in peripheral blood and plasma concentrations of CNI, detection of mass concentration of ATP in CD4+ T cells in peripheral blood is more accurate in immunity monitoring of renal transplant recipients, and can serve as guidance for adjustment of immunosuppressants.

Key words: renal transplant recipient, infection, CD4+ T cells, adenosine triphosphate, immunity monitoring