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    Translational medicine
    2016, 36 (02):  143. 
    Abstract ( 354 )   PDF (1959KB) ( 710 )  
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    LI Xiao team got new progress in mechanism of myelodysplastic syndromes
    2016, 36 (02):  145. 
    Abstract ( 451 )   PDF (389KB) ( 862 )  
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    CHEN Guo-qiang said, “I am still a spring leaf”
    2016, 36 (02):  146. 
    Abstract ( 428 )   PDF (1423KB) ( 840 )  
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    Original article (Basic research)
    Effects of CCL20 on CCR6+ regulatory T cells and pregnancy outcome of CBA/J mice
    ZHANG Xiao-xin, ZHAO Ai-min
    2016, 36 (02):  147. 
    doi: 10.3969/j.issn.1674-8115.2016.02.001

    Abstract ( 853 )   PDF (2377KB) ( 685 )  

    Objective To explore the effects of CCL20 on CCR6+ regulatory T cells and pregnancy outcome of CBA/J mice. Methods The spontaneous abortion mice model CBA/J(♀) × DBA/2(♂) (spontaneous abortion group) and normal pregnant mice model CBA/J(♀) × Balb/c (♂)(normal pregnancy group) were established. The pregnancy outcomes of 2 groups were observed. Real-time PCR, Western blotting, ELISA, and flow cytometry were used to detect the dynamic changes of CCL20 and CCR6+Treg cells at different pregnant stages of CBA/J mice. Normal pregnant mice and spontaneous abortion mice were injected with CCL20 neutralizing antibody and recombined CCL20, respectively, and changes of the pregnancy outcome were observed. The effects of CCL20 and its antibody on changes of chemotaxis, proliferation, and cytokine level of Treg cells were observed in vitro. Results On gestational day 14.5 (gd 14.5), the rates of embryo resorption of spontaneous abortion group and normal pregnancy group were 11.25% and 2.54%, respectively. The difference was statistically significant (P=0.001 8). At the different pregnant stages, the expression levels of CCL20 and CCR6 in peripheral blood and uterus of rats of spontaneous abortion group were lower than those of the normal pregnancy group. At the early stage of pregnancy, especially on gd 2.5, the expression of CCL20-CCR6 and the proportion of Treg cells of normal pregnancy group significantly elevated, while those of spontaneous abortion group didnt. After normal pregnant mice were injected with CCL20 neutralizing antibody on gd 0.5, the embryo resorption rate significantly increased and the proportion of CCR6+ Treg cells significantly decreased. After spontaneous abortion mice were injected with recombined CCL20, the embryo resorption rate decreased and the proportion of CCR6+ Treg cells increased. CCL20 could increase the chemotactic function and proliferative function of Treg cells and the secretion of inhibiting cytokines, which resulted in the balance among T cells leaning towards Th2 and Treg cells and facilitated the maintenance of the pregnancy. Conclusion The spontaneous abortion of CBA/J(♀) × DBA/2(♂) mice may be relevant to the down-regulation of CCL20 and CCR6+Treg cells, especially at the early stage of pregnancy. The early intervention of the protein level of CCL20 can change the quantity and function of CCR6+Treg cells, which can influence the pregnancy outcome.

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    Effects of spleen tyrosine kinase on oxidative stress-induced mucous hypersecretion of airway epithelial cells
    RAN Dan-hua, CHEN Ling-xiu, HUANG Hua-ping, et al
    2016, 36 (02):  156. 
    doi: 10.3969/j.issn.1674-8115.2016.02.002

    Abstract ( 743 )   PDF (1412KB) ( 744 )  

    Objective To explore the effects of spleen tyrosine kinase (Syk) on oxidative stress-induced airway mucous hypersecretion. Methods Human airway epithelial cells 16HBE were cultured in vitro. The mucous hypersecretion model was established by stimulating cells by H2O2. Intervention factors were Syk siRNA and extracellular signal regulated kinase (ERK1/2) specific inhibitor U0126 and P38 specific inhibitor SB203580 of mitogen-activated protein kinase (MAPK) signaling pathway. The cells were randomly divided into the control group, H2O2 group, H2O2+Syk siRNA group, H2O2+negative siRNA group, H2O2+U0126 group, and H2O2+SB203580 group. The cell viability was detected by MTT assay. Transcription and protein expression of mucin (MUC) 5AC were detected by RT-PCR and ELISA respectively. The protein expression of Syk, phosphorylated ERK1/2 (p-ERK1/2), and phosphorylated P38 (p-P38) were detected by Western blotting. The content and distribution of MUC5AC protein were detected by confocal laser technology. Results Compared with the control group, expressions of Syk, p-ERK1/2, p-P38, and MUC5AC significantly increased after being stimulated by H2O2 (P=0.000). Above effects of H2O2 were significantly inhibited after being treated by Syk siRNA, U0126, and SB203580 (P=0.000). Conclusion Syk involves the oxidative stress induced airway mucous hypersecretion via ERK1/2 and P38 MAPK signaling pathways.

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    Effect of Wei Chang An and Si Teng Fang on stem cells CD133+ of human colon cancer strains
    ZHU Li-min, SHEN Ke-ping, ZHOU Hao, et al
    2016, 36 (02):  161. 
    doi: 10.3969/j.issn.1674-8115.2016.02.003

    Abstract ( 764 )   PDF (1300KB) ( 748 )  

    Objective To observe the inhibitory effect of Wei Chang An and Si Teng Fang on colon cancer cell strains HCT-116, SW480, and HT-29 and colon cancer stem cells CD133+. Methods Human colon cancer cell strains HCT-116, SW480, and HT-29 were randomly divided into the control group, WCA group (500 mg/L and 1 000 mg/L), and STF group (100 mg/L and 200 mg/L). After 24 h of intervention, cell viability was detected by CCK-8 assay and cell morphology was observed by the inverted microscope. The effects of WCA and STF on the expression of CD133+ in HCT-116, SW480, and HT-29 were analyzed by flow cytometry. Results The results of CCK-8 assay showed that after HCT-116, SW480, and HT-29 cells were intervened by WCA and STF, their viability decreased in a dose-dependent manner. The observation results of the inverted microscope indicated that after 24 h of intervention by WCA and STF, the number of SW480, HT-29, and HCT-116 cells decreased, the cell volume reduced, light shading weakened, and cells suspended, detached, and died. The results of flow cytometry analysis showed that for the control group, proportions of CD133+ cells in cell strains HCT-116, SW480, and HT-29 were 12.52%, 10.98%, and 2.33%, respectively. After 24h of intervention by WCA of 1 000 mg/L, proportions of CD133+ cells dropped to 7.49%, 5.36%, and 0.11%. After 24 h of intervention by STF of 200 mg/L, proportions of CD133+ cells dropped to 7.83% and 6.45%, and 0.18%. Conclusion Proportions of CD133+ cells in different colon cancer cell strains were different. Both WCA and STF can inhibit the proliferation of colon cancer cell strains HCT-116, HT-29, and SW480. But within the same intervention time, only higher concentration of WCA (1 000 mg/L) and STF (200 mg/L) can inhibit the expression of stem cells CD133+ of the three cell strains.

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    Comparison of electrophysiological properties of selective serotonin reuptake inhibitors in heterologously expressed model of oocytes
    YANG Zhi-fang, ZHANG Ying, LI Ci-zhen, et al
    2016, 36 (02):  166. 
    doi: 10.3969/j.issn.1674-8115.2016.02.004

    Abstract ( 594 )   PDF (1077KB) ( 806 )  

    Objective To observe and explore the inhibitory effect of selective serotonin reuptake inhibitors (SSRIs) citalopram, fluoxetine, and paroxetine and the atypical anti-depressant drug trazodone on the human serotonin transporters (hSERTs). Methods hSERTs were transfected into the oocytes of African Xenopus laevis to establish a heterologous expression system. Two-electrode voltage clamp technique was used to record the changes of 5-HT-induced transport current under the action of different drugs. Results The same concentration of paroxetine, citalopram, fluoxetine, and trazodone inhibited the 5-HT-induced initial transport current by (29.31±1.52)%, (47.17±3.38)%, (27.72±2.01)%, and (43.30±2.83)%, respectively. But trazodone failed to efficiently reverse the 5-HT-induced transport current within its action time. The binding time constants between drugs and hSERTs and competition time constants between drugs and 5-HT were calculated by fitting current curves with the exponential decay function. Among three SSRIs, binding and competition time constants of paroxentine were the smallest, followed by fluoxentine and citalopram. Conclusion The initial inhibition effect of paroxetine on 5-HT-induced transport current is better than that of fluoxentine and citalopram.

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    Expression and distribution of dCTP pyrophosphatase 1 in gastric cancer tissues and its clinical relevance
    XU Ling, XIA Li-liang, SONG Fei-fei, et al
    2016, 36 (02):  172. 
    doi: 10.3969/j.issn.1674-8115.2016.02.005

    Abstract ( 598 )   PDF (2945KB) ( 739 )  

    Objective To detect the expression and distribution of dCTP pyrophosphatase 1 (DCTPP1) in gastric cancer tissues, analyze the correlation between DCTPP1 and clinicopathological characteristics of gastric cancer, and explore possible mechanisms of the involvement of DCTPP1 in the occurrence and development of gastric cancer. Methods The normal, adjacent, and tumor tissues of patients with gastric cancer were collected. The expression of DCTPP1 in these tissues was compared by Western blotting. The distribution of expression of DCTPP1 in gastric cancer cells was detected by immunohistochemical method and its correlation with clinical pathological characteristics of gastric cancer was explored. The expression and distribution of DCTPP1 of gastric cancer with different stages were analyzed. Results For three types of tissues of the same patient, the expression of DCTPP1 in gastric cancer tissue was significantly higher than that of normal and adjacent tissues. Results of the histological observation indicated that DCTPP1 expressed in cytoplasm and nucleus of gastric cancer tissue cells. The expression of DCTPP1 in nucleus increased with the TNM stage. The expression of DCTPP1 in nucleus of gastric cancer tissues of patients with lymph node metastasis was higher than that of patients without lymph node metastasis. Conclusion Compared with normal and adjacent tissues, the expression of DCTPP1 in gastric cancer tissues is higher. The high expression of DCTPP1 in nucleus of gastric cancer cells statistically correlates with the severity and metastasis of tumors to a certain extent. Since DCTPP1 participates in regulating the metabolism of abnormal nucleotides, its high expression in tumor cells and the accumulation in nucleus may play an important role in the occurrence and development of gastric cancer.

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    Effects of BMSCs conditioned medium on ratio of MMP-9 to TIMP-1 in lung tissues of rats with pulmonary fibrosis induced by bleomycin
    XIONG Jian-fei, LI Yan-qin, XU Jia-bo, et al
    2016, 36 (02):  178. 
    doi: 10.3969/j.issn.1674-8115.2016.02.006

    Abstract ( 632 )   PDF (2831KB) ( 764 )  

    Objective To observe the effects of bone marrow-derived meschymal stem cells (BMSCs) conditioned medium on pulmonary fibrosis of rats. Methods Fifty SD rats were randomly divided into normal control group, model group, and treatment group (A, B, and C). Rats of the model group and treatment groups were intratracheally perfused with bleomycin (5 mg/kg) and rats of the control group were intratracheally perfused with normal saline. Rats of the model group were injected with DMEM/F-12 concentrated culture medium via caudal vein on the next day. Rats of treatment groups were injected with different volumes of BMSCs conditioned medium via caudal vein. Lung tissues of rats were harvested after 20 d and hematoxylin-eosin (H-E) staining and Masson staining were performed. The hydroxyproline (HYP) level and changes of mRNA expressions of MMP-9 and TIMP-1 in lung tissues and levels of MMP-9 and TIMP-1 proteins in lung tissue homogenate were detected. Results Compared with the model group, alveolitis and pulmonary fibrosis of three treatment groups alleviated and the level of HYP decreased. The mRNA level of MMP-9 in lung tissues and the protein level of MMP-9 and the ratio of MMP-9 to TIMP-1 in lung tissue homogenate of treatment groups were higher than those of the model group and control group. Conclusion The BMSCs conditioned medium can alleviate alveolitis and pulmonary fibrosis induced by bleomycin via increasing the level of MMP-9 and the ratio of MMP-9 to TIMP-1.

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    Effects of color of abutment and resin cement on color of CAD/CAM all-ceramic veneers
    DU Yi-min, CHEN Li-Ping, SHE Wen-jun, et al
    2016, 36 (02):  184. 
    doi: 10.3969/j.issn.1674-8115.2016.02.007

    Abstract ( 622 )   PDF (1354KB) ( 856 )  

    Objective To investigate the effect of the color of resin cement on the color of CAD/CAM lithium disilicate ceramic veneers. Methods Different colors (ND1-ND7) of abutment specimens and IPS e.max CAD LTA1 0.7 mm ceramic slices were prepared and cemented by resin cement Variolink N (four different colors). The color of veneers was measured. The color difference (ΔE) between the color of veneers and standard color (2 mm LTA1, LTA2 lithium disilicate ceramic blocks) was calculated. ANOVA analysis and Tukeys HSD test were performed for multiple comparisons. Results ΔE values were smaller than the clinical acceptance level. The differences of the following colors were not statistically significant: white, yellow, and bleach XL of the ND1 group (P=0.999, 0.969, and 0.991); colors of the ND2 group (df=3, F=2.865, P=0.069); white and yellow of the ND4 group (P=0.819); white, transparent color, and yellow of the ND5 group (P=1.000, 0.998, 1.000); and transparent color, white, and yellow of the ND6 group (P=0.487,0.169,0.883). For the ND3 group, only ΔE values of the yellow color were smaller than the clinical acceptance level and the differences between the ND3 group and other groups were statistically significant (P<0.05). ΔE values of all colors of the ND7 group were larger than the clinical acceptance level. Conclusion Under limited conditions, the yellow color of veneers is insufficient after cemented by Veriolink N resin cement and the adjustment of yellow color is needed. Bleach XL resin cement will turn the veneer color towards cool color tone and yellow resin cement will turn the veneer color towards warm color tone. The effect of white and transparent resin cement is between that of bleach XL and yellow resin cement.

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    Effects of irbesartan on left ventricular hypertrophy and expression of urea transporter B in myocardium of 5/6 nephrectomized rats with constricted abdominal aorta
    LIU Yi-sheng, LU Wei, HUANG Hai-dong, et al
    2016, 36 (02):  189. 
    doi: 10.3969/j.issn.1674-8115.2016.02.008

    Abstract ( 681 )   PDF (1803KB) ( 828 )  

    Objective To observe the effects of angiotensin Ⅱ receptor antagonist irbesartan on left ventricular hypertrophy and the expression of urea transporter B (UT-B) in myocardium of 5/6 nephrectomized rats with constricted abdominal aorta. Methods SD rats were randomly divided into the sham group, 5/6 nephrectomy group, abdominal aorta constriction group, 5/6 nephrectomy and abdominal aorta constriction group, and irbesartan group. Each group consisted of eight rats. Serum Cr, BUN, AngⅡ, and BNP were detected after rats were sacrificed. Rat hearts were harvested and the left ventricular weight/heart weight (LVW/HW), heart weight/body weight (HW/BW), and left ventricular weight/body weight (LVW/BW) were measured. The expression of UT-B in myocardium was determined by RT-PCR and Western blotting. Results Compared with the sham group, rats of 5/6 nephrectomy group, abdominal aorta constriction group, and 5/6 nephrectomy and abdominal aorta constriction group had the left ventricular hypertrophy and elevated LVW/HW, HW/BW, LVW/BW, serum AngⅡ, BNP, and mRNA and protein expressions of UT-B in myocardium. Levels of SCr and BUN of the 5/6 nephrectomy group and 5/6 nephrectomy and abdominal aorta constriction group increased. Compared with above three groups, rats of the irbesartan group had alleviated left ventricular hypertrophy and lower LVW/HW, HW/BW, LVW/BW, serum AngⅡ, BNP, and mRNA and protein expressions of UT-B in myocardium. Compared with the 5/6 nephrectomy group and 5/6 nephrectomy and abdominal aorta constriction group, levels of SCr and BUN of the irbesartan group were lower. Conclusion 5/6 nephrectomy and abdominal aorta constriction can cause significant decrease of heart function of rats, left ventricular hypertrophy, and elevated expression of UT-B. Irbesartan can improve heart and kidney functions and inhibit the expression of UT-B, which may play an important role in heart failure of rats with renal inadequacy.

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    Correlation between metastasis-associated protein 3 and prognosis of patients with breast cancer
    WANG Cheng, HONG Yan, YU Yan-min, et al
    2016, 36 (02):  195. 
    doi: 10.3969/j.issn.1674-8115.2016.02.009

    Abstract ( 650 )   PDF (2026KB) ( 818 )  

    Objective To investigate the correlation between metastasis-associated protein 3 (MTA3) and the prognosis of patients with breast cancer. Methods Tissue microarrays were made using 160 breast cancer samples and contained epidermal growth factor receptor (EGFR) data, estrogen receptor (ER) data, and follow-up information. The expression of MTA3 protein was detected by immunohistochemical method and the correlation between the expression of MTA3 and the prognosis was analyzed. Results For EGFR+/ER- breast cancer, the overall survival of patients with high expression of MTA3 in cancer tissues was longer (67.9% and 41.2%, P=0.059). For EGFR-/ER+ breast cancer, the overall survival of patients with low expression of MTA3 in cancer tissues was longer (100.0% and 72.0%, P=0.039). For EGFR+/ER+ breast cancer, the expression of MTA3 in cancer tissues did not correlate to the prognosis (P=0.405). Conclusion The function of MTA3 may be bi-directionally regulated by EGFR and ER pathways, so as to affect the prognosis of patients with breast cancer.

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    Expressions of CIP2A and CK2β in tissues of esophageal squamous cell carcinoma and the correlation with prognosis
    LI Hong-wei, LI Duo-jie, CHAI Da-min, et al
    2016, 36 (02):  200. 
    doi: 10.3969/j.issn.1674-8115.2016.02.010

    Abstract ( 764 )   PDF (1731KB) ( 860 )  

    Objective To investigate the significance of expressions of phosphatase 2A (CIP2A) and kinase 2 beta (CK2β) in tissues of esophageal squamous cell carcinoma (ESCC) and the effect on the prognosis of patients. Methods Immunohistochemical staining was used to detect the expressions of CIP2A and CK2β in 155 tissue samples of ESCC (experiment group) and 50 adjacent normal mucous membrane tissues (control group). The correlation between the expressions of CIP2A and CK2β and the clinical pathological parameters was analyzed. Kaplan-Meier survival analysis and Cox multivariate analysis were adopted to analyze the survival of patients with ESCC. Results The positive rates of expressions of CIP2A and CK2β in tissues of ESCC were 76.67% and 66.45%, which were remarkably higher than 16.00% and 12.00% of the control group. The differences were statistically significant (P=0.000). CIP2A was correlated with lymph node metastasis and TNM stage of patients with ESCC (P=0.005, P=0.000), while was not correlated with age, gender, histological grade, and depth of invasion. The expression of CK2β was correlated with histological grade, lymph node metastasis, and TNM stage of patients with ESCC (P=0.000,P=0.004,P=0.000), while was not correlated with age, gender, and depth of invasion. The expression of CIP2A was positively correlated with the expression of CK2β (r=0.611,P=0.000). The overall 5-year survival rate of patients with ESCC was 23.33%. The 5-year survival rate of patients with positive expression of CIP2A was 8.11% (9/111) and that of patients with negative expression of CIP2A was 66.67% (26/39). The differences were statistically significant (P=0.000). The 5-year survival rate of patients with positive expression of CK2β was 6.06% (6/99) and that of patients with negative expression of CK2β was 56.86% (29/51). The differences were statistically significant (P=0.000). Cox multivariate analysis revealed that the TNM stage and expression levels of CIP2A and CK2β were independent risk factors of influencing the prognosis of patients with ESCC (P=0.008,P=0.013, P=0.000). Conclusion Expressions of CIP2A and CK2β are closely related with the occurrence and development of ESCC and may involve in the invasion and metastasis of ESCC. The prognosis of patients with positive expressions is poor. Combined detection of expressions of CIP2A and CK2β is vital for predicting the prognosis of patients with ESCC.

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    Therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms
    YE Fei, LU Xiao-xiao, LIU Zi-yu, et al
    2016, 36 (02):  206. 
    doi: 10.3969/j.issn.1674-8115.2016.02.011

    Abstract ( 727 )   PDF (966KB) ( 648 )  

    Objective To investigate the therapeutic effect of Jinlida granules on the liver injury of mice with type 1 diabetes mellitus and possible mechanisms. Methods The SD mouse model of type 1 diabetes mellitus was established by a single intraperitoneal injection of streptozotocin (STZ). The model rats were randomly divided into the model group (DM group), 0.75 g/kg, 1.5 g/kg, and 3.0 g/kg therapy groups. A normal control group (NC group) was established and there were 10 rats in every group. The fasting glucose (FBG), HbA1C, insulin, C-peptide, liver function indexes, hepatic fibrosis indexes, oxidative stress indexes, and inflammation indexes were detected and pathological changes of liver tissues were observed 8 weeks after the administration. Results Compared with the NC group, levels of FBG, liver enzyme, hepatic fibrosis indexes, oxidative stress indexes, and inflammation factors of the DM group increased. Levels of GPT, GOT, AKP, HA, IV-C, PC-Ⅲ, LN, SOD, MAD, NO, and ROS of the 3.0 g/kg therapy group decreased after administration, while levels of GSH-PX and CAT increased. Levels of CRP and IL-6 of three therapy groups decreased significantly and the hepatic fibrosis alleviated. Conclusion Jinlida granules have protective effect on the liver injury of mice with type 1 diabetes mellitus. The mechanism may be relevant to the decrease of oxidative stress reaction and alleviation of inflammation.

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    Effects of gestational diabetes mellitus on cardiac contractile function of female offspring rats after ischemia/reperfusion and underlying mechanisms
    ZOU Hong, JIANG Xiao-xue, WANG Han-bing, et al
    2016, 36 (02):  211. 
    doi: 10.3969/j.issn.1674-8115.2016.02.012

    Abstract ( 573 )   PDF (2249KB) ( 659 )  

    Objective To investigate the effects of gestational diabetes mellitus (GDM) on the cardiac function of female offspring rats and underlying mechanisms by the rat model. Methods Wild type female SD rats were injected with streptozotocin (35 mg/kg) in the second trimester of pregnancy to establish the GDM model (GDM group). The control group was also established. Female F1 offspring rats were fed for 24 weeks after they were born. During this period, random blood glucose level, blood pressure, and heart rate were measured and glucose tolerance test and insulin tolerance test were conducted. The in vitro myocardial ischemia/reperfusion (I/R) model was established. The signaling pathways of protein kinase B (Akt), adenine monophosphate activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in cardiac tissues were detected by Western blotting. Results The in vitro cardiac contractile function of female F1 offspring of the GDM group after I/R was significantly weaker than that of the control group (P=0.048 6). The results of Western blotting showed that the phosphorylation of AMPK and ACC in cardiac tissues of female F1 offspring of the GDM group after in vitro I/R was significantly lower than that of the control group (P=0.005 6). Conclusion GDM results in cardiac I/R intolerance of female offspring rats, which may be relevant to the blunt response to I/R stimulation caused by the phosphorylation of AMPK.

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    Effects of silencing FUNDC1 gene on proliferation and invasion of ovarian cancer cell SKOV3
    LONG Ying-fei, TANG Jun-ying
    2016, 36 (02):  218. 
    doi: 10.3969/j.issn.1674-8115.2016.02.013

    Abstract ( 624 )   PDF (2819KB) ( 682 )  

    Objective To explore the expression of FUNDC1 protein in tissues of ovarian cancer and its effects on proliferation and invasion of ovarian cancer cells. Methods The expression of FUNDC1 protein in tissues of ovarian cancer was detected by immunohistochemical (S-P method). Changes of the cell cycle of ovarian cancer cell SKOV3 were detected by flow cytometry after the FUNDC1 gene was silenced. The abilities of proliferation and invasion of ovarian cancer cell SKOV3 were detected by EdU assay and Transwell assay, respectively. Western blotting was used to detect the expressions of p21 and p53 proteins. Results FUNDC1 expressed in the cytoplasm of ovarian cancer tissues. FUNDC1 positively expressed in tissues of ovarian cancer, while showed weakly positive or negative expression in normal ovarian tissues. Compared with normal ovarian tissues, the expression of FUNDC1 in ovarian cancer tissues was significantly higher (P=0.021). The expression of FUNDC1 in advanced ovarian cancer tissues (stage Ⅲ/Ⅳ) was significantly higher that in early stage ovarian cancer tissues (stage Ⅰ/Ⅱ) and the difference was statistically significant (P=0.012). After the FUNDC1 gene of ovarian cancer cell SKOV3 was silenced, the abilities of proliferation (P=0.032) and invasion (P=0.035)of ovarian cancer cells decreased. Cells at S phase decreased significantly (P=0.015) and expressions of p21 and p53 proteins up-regulated. Conclusion The expression of FUNDC1 protein in ovarian cancer tissues is up-regulated. The regulation of proliferation of ovarian cancer cell SKOV3 by FUNDC1 may be relevant to p21/p53 pathway.

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    Preliminary study on the synthesis and biological activity of small molecule #br# inhibitor JK043 of SUMO-specific protease 1
    CHEN Ying-yi, SUN Jin-ling, LI Shuai, et al
    2016, 36 (02):  224. 
    doi: 10.3969/j.issn.1674-8115.2016.02.014

    Abstract ( 1008 )   PDF (1333KB) ( 739 )  

    Objective To identify novel small molecule inhibitors of SENP1 by virtual screening and evaluate the biological activity in vitro. Methods The possible small molecule inhibitors of SENP1 were selected from the small molecule database by virtual screening. Screened JK043 were chemically synthesized and the inhibitory effect of JK043 on the activity of SENP1 was evaluated by the in vitro test system of SENP1. IC50 was calculated. The action mode between JK043 and SENP1 was then observed through the docking model. Results The activity test in vitro showed that chemically synthesized JK043 could inhibit the activity of SENP1 and the valued of IC50 was 1.339 μmol/L. The docking model indicated that JK043 interacted with 465W, 468D, 529H, and 597Q of SENP1 protein. Conclusion JK043 has been identified and synthesized, which can inhibit the activity of SENP1 in vitro to a certain extent.

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    Correlation between polymorphism of resistin gene and cerebral infraction
    GU Lin, CAI Gao-yu, CHEN Chun, et al
    2016, 36 (02):  229. 
    doi: 10.3969/j.issn.1674-8115.2016.02.015

    Abstract ( 524 )   PDF (546KB) ( 664 )  

    Objective To investigate the correlation between the polymorphism of locus -420C/G of the resistin gene and cerebral infraction. Methods The polymorphism of locus -420C/G of the resistin gene of 426 patients with cerebral infraction and 305 healthy controls was analyzed by PCR-LDR. Results The difference of TC between the cerebral infraction group and control group were not statistically significant. The TG, LDL-C, fasting blood glucose, and blood pressure of the cerebral infraction group were higher than those of the control group, while HDL-C was lower than that of the control group. The differences of genotype frequency and allele frequency of -420C/G gene between the cerebral infraction group and control group were not statistically significant. Samples of both groups accorded with the H-W test. The logistic regression analysis showed that sytolic pressure, fasting blood glucose, LDL-C, and TG were independent risk factors of cerebral infraction. Conclusion The polymorphism of locus -420 C/G of the resistin gene does not correlate to cerebral infraction.

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    Original article (Clinical research)
    Analysis of the correlation between the severity of coronary artery lesions and levels of glycated hemoglobin and bilirubin for patients with diabetes and coronary atherosclerotic cardiopathy
    LI Hui-hua, LV Hui, LU Jian-can, et al
    2016, 36 (02):  233. 
    doi: 10.3969/j.issn.1674-8115.2016.02.016

    Abstract ( 688 )   PDF (671KB) ( 705 )  

    Objective To explore the correlation between the severity of coronary artery lesions and levels of glycated hemoglobin (HbA1c) and bilirubin (TBI) for patients with diabetes and coronary atherosclerotic cardiopathy (coronary heart disease for short). Methods A total of 124 patients with diabetes and coronary heart disease who treated in Shanghai Changhai Hospital from March 2010 to December 2014 were selected. Patients were divided into single-vessel lesion group (41 cases), double-vessel lesion group (42 cases), and three-vessel lesion group (41 cases) according to the severity of coronary artery lesions. The differences of HbA1c and TBI levels among three groups were compared. The correlation between levels of HbA1c and TBI and the severity of coronary artery lesions was analyzed. Results The differences of age, BMI, TC, TG, LDL, HDL, diabetes duration, HbA1c, fasting glucose, and TBI of three groups were statistically significant (P<0.05). The multivariate logistic regression analysis showed that duration of diabetes, HbA1c, and bilirubin were independent risk factors of the severity of coronary artery lesions (P<0.05). The severity of coronary artery lesions of patients with diabetes and coronary heart disease positively correlated to the level of HbA1c (r=0.337, P=0.000) and negatively correlated to the level of TBI (r=-0.372, P=0.000). Conclusion The severity of coronary artery lesions of patients with diabetes and coronary heart disease positively correlates to the level of HbA1c and negatively correlates to the level of TBI.

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    Elastin gene mutation screening for patients with supravalvular aortic stenosis
    LIU Yang, XU Li-juan, SUN Kun
    2016, 36 (02):  237. 
    doi: 10.3969/j.issn.1674-8115.2016.02.017

    Abstract ( 753 )   PDF (974KB) ( 721 )  

    Objective To screen the elastin gene (ELN) mutations of patients with Williams syndrome (WS) or isolated supravalvular aortic stenosis (SVAS) and analyze the causes of cardiovascular variability among patients with WS and the genetic causes of the isolated SVAS. Methods Eight patients with WS and six patients with isolated SVAS were enrolled. Multiplex ligation-dependent probe amplification (MLPA) was adopted to detect the microdeletion. PCR was applied to amplify all exons of ELN gene. Direct forward and reverse sequencing was performed for all amplified fragments. The results of sequencing were compared with the sequence of elastin in GenBank by the BLAST program and possible mutations were screened. Results The microdeletion in 7q11.23 region was present in all eight patients with WS and absent in all patients with isolated SVAS and the deletion size was 1.04-1.61Mb. Among seven detected SNPs, five of them located in the intron region without involving splice-site, while another two SNPs located in exon region. The mutation c.212 C>T was detected in No. 5 exon of No.01 patient and a synonymous mutation c.1674G>A was identified in No. 25 exon of No.07 patient. Conclusion The causes of cardiovascular variability of patients with WS and the genetic causes of the isolated SVAS are complicated and need more extensive gene screening.

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    Clinical features of electrical storm of ventricular tachycardia of patients with implantable cardioverter defibrillator
    CAO Dong-lai, LI Chang, WANG Yi-Long, et al
    2016, 36 (02):  243. 
    doi: 10.3969/j.issn.1674-8115.2016.02.018

    Abstract ( 578 )   PDF (719KB) ( 909 )  

    Objective To analyze the clinical features and treatment of electrical storm (ES) of ventricular tachycardia after operation for patients with implantable cardioverter defibrillator/cardiac resynchronization therapy defibrillator (ICD/CRT-D). Methods Eighty-nine patients with ICD/CRT-D were followed up regularly to collect relevant data and analyze the onset characteristics of electrical storm of ventricular tachycardia, related risk factors, and corresponding treatment outcomes. Results Nineteen patients (21.3%) experienced at least one episode of ES during the follow up. Among them, 11 patients experienced two or more episodes of ES. 14 patients (73.7%) experienced the first episode of ES within one year after ICD operation. Monomorphic ventricular tachycardia (VT)(68.4%) was the main arrhythmic type of ES and no obvious causes were identified for the onset of ES of most patients (68.4%). The multivariate logistic regression analysis showed that as the secondary prevention of sudden cardiac death, ICD was an independent risk factor of the onset of ES (P=0.014 2). The onset times of ES of patients with coronary atherosclerotic cardiopathy and dilated cardiomyopathy implanted with CRT-D was less than those of patients implanted with ICD (P=0.033). The mortality rate of ES group was not significantly higher. Conclusion ES is common for patients with ICD. The incidence of ES of patients with ICD for secondary prevention of sudden cardiac death is significantly higher than that of patients for primary prevention. The onset of ES of patients with coronary atherosclerotic cardiopathy and dilated cardiomyopathy who have implanted the CRT-D is less common.

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    Effects of estradiol pretreatment at luteal phase on outcome of in vitro fertilization and embryo transfer treatment of antagonist protocol for patients with poor ovarian responder
    LU Xiang, XI Ji, JIANG Shan, et al
    2016, 36 (02):  248. 
    doi: 10.3969/j.issn.1674-8115.2016.02.019

    Abstract ( 929 )   PDF (632KB) ( 745 )  

    Objective To explore the effects of estradiol pretreatment at the luteal phase on the outcome of in vitro fertilization and embryo transfer (IVF-ET) treatment of antagonist protocol for patients with poor ovarian responder. Methods Data of 173 patients with poor ovarian response who have underwent IVF-ET treatment were retrospectively analyzed. Patients were divided into the pretreatment group (n=96) and non-pretreatment group (n=77) according to whether the estradiol pretreatment (oral administration with 17 β-estradiol for 4 mg/d at the luteal phase till the second day of menstrual cycle) were conducted before super ovulation induction. General information and indexes relevant to the outcome of IVF-ET treatment of two groups were compared. Results The differences of age (P=0.857), BMI (P=0.727), basal serum FSH (P=0.926)/LH (P=0.579)/E2 value (P=0.898), basal antral follicle count (AFC) (P=0.662), endometrial thickness (P=0.542) and the number of embryos transferred (P=0.321) on transfer day between the two groups were not statistically significant. Compared with the non-pretreatment group, blood FSH (P=0.000) and LH (P=0.019) levels of the pretreatment group before super ovulation induction significantly decreased, mean antral follicle sizes significantly decreased (P=0.000), the total amount of gonadotropin (P=0.001) and stimulation period (P=0.001) significantly increased, and HCG daily E2 (P=0.000) and LH level (P=0.000) increased. The number of retrieved oocytes (P=0.001) and obtained embryos (P=0.005) of the pretreatment group was larger than that of the non-pretreatment group, while the differences of fertilization rate (P=0.648), high-quality embryo rate (P=0.172), implantation rate (P=0.845), clinical pregnancy rate (P=0.948), and continued pregnancy rate (P=0.899) between two groups were not statistically significant. Conclusion Estradiol pretreatment at the luteal phase can increase the number of retrieved oocytes and obtained embryos during the IVF-ET treatment of antagonist protocol for patients with poor ovarian responder.

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    Determination of the minimum local analgesic concentration of ropivacaine for the application of ropivacaine combined with sufentanil to epidural labor analgesia by sequential allocation method
    SHEN Ting, ZHENG Jing
    2016, 36 (02):  252. 
    doi: 10.3969/j.issn.1674-8115.2016.02.020

    Abstract ( 577 )   PDF (724KB) ( 712 )  

    Objective To determine the minimum local analgesic concentration (MLAC) of ropivacaine for the application of ropivacaine combined with sufentanil to epidural analgesia at the first and the second stage of labor by sequential allocation method and observe its adverse reactions. Methods A total of 30 nulliparous full-term primiparas were enrolled. The epidural analgesia was performed when cervical dilation reached 3 cm at the first stage of labor. The initial concentration of ropivacaine was 0.1% combined with sufentanil of 0.3 μg/mL. According to the response of the previous primipara, the concentration of ropivacaine was increased or decreased by 0.01% for the next primipara. The analgesia was defined as effective if the visual analogue scale (VAS) score was no more than 3 from 30 min after administration to the end of the second stage of labor. The Brownlee up-and-down sequential allocation was used to calculate the MLAC and 95%CI of ropivacaine. Results For epidural analgesia at the first and the second stage of labor, the MLAC and 95%CI of ropivacaine were 0.092 3% and 0.091 8%-0.092 8%. Five primiparas had PONV and the incidence was 16.7%. One primipara had pruritus and the incidence was 3.3%. Conclusion The MLAC of ropivacaine is 0.092 3% when applying ropivacaine with sufentanil of 0.3 μg/mL to epidural analgesia at the first and the second stage of labor.

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    Direct identification of positive blood culture specimens by MSK-MS method
    LI Yuan-rui, YU Jing, LIU Jing-xian, et al
    2016, 36 (02):  256. 
    doi: 10.3969/j.issn.1674-8115.2016.02.021

    Abstract ( 821 )   PDF (848KB) ( 711 )  

    Objective To evaluate the feasibility and clinical significance of applying MALDI SepsityperTM Kit and MicroflexTM LT matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MSK-MS method) to direct identification of microorganisms in positive blood culture specimens. Methods From March to August 2014, both MSK-MS method and traditional blood culture identification method were adopted to identify microorganisms in positive blood culture specimens collected in Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine. If identified results of two methods were inconsistent, the gene sequencing was adopted to make confirmation. Results Among 491 positive blood culture specimens, 462 of them were monobacterial specimens and 29 of them were polymicrobial specimens. There were a total of 30 genera and 64 species or groups. For monobacterial specimens, the coincidence rates of MSK-MS method for identifying genera and species were 72.5% (335/462) and 76.0% (351/462), respectively. The coincidence rates of MSK-MS method for identifying genera and species of Gram-negative bacteria, Gram-positive bacteria, and Candida were 75.4% (132/175) and 77.7% (136/175), 70.9% (178/251) and 74.9% (188/251), and 75.0% (27/36) and 75.0% (27/36), respectively. For polymicrobial specimens, the coincidence rates for identifying one and two bacteria were 55.2% (16/29) and 20.7% (6/29), respectively. The coincidence rates of MSK-MS method for identifying genera of common clinical bacteria of the bloodstream infection were between 74.2% and 96.9%. The identification time was only 40 min per specimen. Conclusion MSK-MS method can accurately and rapidly identify common bacteria of the bloodstream infection, so as to shorten the time for diagnosing the bloodstream infection and provide evidences for quick and rational use of antibiotics.

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    Effects of serum 25(OH)D3 and lipid levels at the second trimester on gestational diabetes mellitus
    ZHOU Ye, GU Wei, LIN Jing
    2016, 36 (02):  264. 
    doi: 10.3969/j.issn.1674-8115.2016.02.022

    Abstract ( 759 )   PDF (797KB) ( 820 )  

    Objective To investigate the effects of serum 25(OH)D3 and lipid levels on the gestational diabetes mellitus (GDM). Methods Pregnant women who underwent prenatal examinations were enrolled and divided into the normal pregnancy group (control group, n=6 796) and GDM group (n=977). Serum 25(OH)D3, total cholesterol (TCh), triglyceride (TAG), high density lipoprotein cholesterol (HDL), and low density lipoprotein (LDL) levels of two groups at the second trimester of pregnancy (12-16 weeks) were detected. The logistic regression analysis was adopted to evaluate the effects of 25(OH)D3 and lipid levels on the pathogenesis of GDM. Results 25(OH)D3 and HDL levels of the GDM group were lower than those of the control group, while TAG, TCh, and LDL levels were higher than those of the control group. Logistic regression analysis showed that all indexes were relevant to the pathogenesis of GDM except LDL. TAG (OR=1.54) and TCh (OR=1.63) were risk factors of GDM, while 25(OH)D3 (OR=0.995) and HDL (OR=0.392) were protective factors of GDM. Conclusion Serum 25(OH)D3 and lipid levels at the second trimester play an important role in the pathogenesis of GDM. TAG and TCh are risk factors of GDM, while 25(OH)D3 and HDL are protective factors of GDM. Therefore, it can be attempted to reduce the incidence of GDM by the combination therapy of lowering lipids and glucose.

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    Correlation between coronary and iliac atherosclerosis
    HUA Sha, ZHAO Jian-rong, QIU Xiao-wei
    2016, 36 (02):  269. 
    doi: 10.3969/j.issn.1674-8115.2016.02.023

    Abstract ( 713 )   PDF (789KB) ( 658 )  

    Objective To explore the correlation between the coronary and iliac artery atherosclerosis by simultaneously conducting iliac angiography and coronary angiography (CAG). Methods A total of 288 patients who underwent iliac angiography immediately after coronary angiography were enrolled. The detection rate and risk factors of iliac atherosclerosis and their correlation with coronary atherosclerosis and its severity were analyzed. Results The mean age of the patients was (66.64±10.58) years. The total detection rate of iliac atherosclerosis was 42.7% (123/288). The incidence of iliac atherosclerosis of patients with coronary atherosclerosis was 54.8%, which was significantly higher than that (23.4%) of patients without coronary atherosclerosis (P=0.000). The result of risk factor analysis showed that elder, male, smoking, hypertension, diabetes mellitus, and hypercholesterolemia were risk factors of iliac atherosclerosis. The iliac atherosclerosis significant correlated with coronary atherosclerosis and its severity (the number of involved vessels) (P=0.001 and 0.000). Conclusion Risk factors of iliac atherosclerosis are similar to those of coronary atherosclerosis and iliac atherosclerosis also correlates with the severity of coronary atherosclerosis.

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    Preliminary investigation of treating patients with critical limb ischemia by staged endovascular technology
    LIU Guang, LI Wei-min, LU Xin-wu, et al
    2016, 36 (02):  274. 
    doi: 10.3969/j.issn.1674-8115.2016.02.024

    Abstract ( 637 )   PDF (1041KB) ( 691 )  

    Objective To investigate the application of staged endovascular treatment to high-risk patients with critical limb ischemia (CLI). Methods From September 2013 to March 2015, a total of 16 patients with CLI (both iliac artery and distal arterial occlusion, TASC-ⅡD) underwent the staged endovascular angioplasty. Clinical data of patients were collected and retrospectively analyzed. The average follow-up period was 7.8 months. Results The technical success was achieved for all patients. The rest pain of patients disappeared after the first endovascular treatment with no amputation and serious complications. During two treatments, the difference of the ankle brachial index (ABI) before and after surgery was statistically significant (P=0.000). Conclusion The staged endovascular technology can effectively alleviate the symptoms of rest pain and tissue necrosis of high-risk patients with CLI, avoid serious complications such as the ischemia reperfusion injury and acute cardiovascular events caused by unstaged endovascular treatment, and improve the life quality of patients.

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    Gender difference of long term prognosis of patients with diastolic heart failure
    YU Tong-tong, WANG Chuan-he, LIU Shuang-shuang, et al
    2016, 36 (02):  278. 
    doi: 10.3969/j.issn.1674-8115.2016.02.025

    Abstract ( 623 )   PDF (1058KB) ( 690 )  

    Objective To investigate the gender difference of long term prognosis of patients with diastolic heart failure. Methods A total of 562 patients with diastolic heart failure were consecutively enrolled and 297 (52.8%) of them were female. The average follow-up time was 3.7 years. The primary end point was all-cause mortality. Cox regression analysis was adopted to investigate the gender difference of long term prognosis of patients with diastolic heart failure. Results Compared with male patients, female patients were older with higher systolic blood pressure and higher incidences of diabetes and anemia, but less smokers. The echocardiography examination showed that the end-diastolic volume and end-systolic volume of females were lower. The laboratory examination indicated that levels of total cholesterol, lower density lipoprotein, higher density lipoprotein and serum sodium of females were higher, while the red blood cell count and levels of hemoglobin, pre-albumin, alanine aminotransferase, serum creatinine, uric acid, urea nitrogen, serum potassium, cardiac troponin Ⅰ, and creatine kinase MB were lower. The differences of medications between males and females after hospital discharge were not statistically significant. During the follow-up, the all-cause mortality of all patients, female patients, and male patients were 24.0%, 23.9%, and 24.2%, respectively. Kaplan-Meier survival analysis (Log Rank, P=0.924), Cox univariate regression analysis (HR=1.016, 95%CI=0.725-1.425, P=0.924), and multivariate regression analysis (OR=0.922, 95%CI=0.571-1.490, P=0.741) after all clinical factors were corrected indicated that the long term survival rate of males was not significantly different from that of females. Cox univariate regression analysis of different genders showed that predictive factors of the elevated all-cause mortality of males were higher age, higher NYHA status, higher heart rate on admission, higher B-type natriuretic peptide, lower serum sodium, lower hemoglobin, lower albumin, renal insufficiency, and higher uric acid. Predictive factors of the elevated all-cause mortality of females were higher age, higher NYHA status, higher heart rate on admission, higher B-type natriuretic peptide, lower hemoglobin, lower serum sodium, and lower albumin. Conclusion The gender difference of long term prognosis of patients with diastolic heart failure is not significant. But the independent predictive factors of patients with different genders are different, thus males and females should be treated accordingly.

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    Original article (Public health)
    Study on equity of allocating health resources of Chongqing
    TAO Yi, ZHONG Xiao-ni, WEN Xiao-yan
    2016, 36 (02):  285. 
    doi: 10.3969/j.issn.1674-8115.2016.02.026

    Abstract ( 762 )   PDF (1038KB) ( 816 )  

    Objective To analyze the status of allocation of health resources in Chongqing and provide scientific evidences for further optimize the allocation of health resources based on the demand and supply. Methods Grey relational analysis was adopted to analyze the demand and supply of health resources of 38 districts and counties in Chongqing. Results The 38 districts and counties were divided into 4 categories according to relative demand and supply of health resources, i.e. category one: Yuzhong, Nanan, Jiangbei, etc; category two: Wulong, Chengkou, Rongchang, etc; category three: Wanzhou, Banan, Yubei, etc; and category four: Dadukou, Yunyang, Dianjiang, etc. Analysis results showed that relative demand and supply of health resources in Chongqing was imbalanced and the allocation of health resources was inequitable. Conclusion The allocation of health resources in Chongqing is inequitable among districts and counties. The classification result based on the demand and supply analysis of health resources in Chongqing is basically consistent with functional classification of districts and counties of Chongqing. The allocation of health resources in Chongqing is inequitable among districts and counties and the structure is unreasonable. Most health resources locate in districts and counties with developed social economy and fewer health resources are available for people in districts and counties with undeveloped social economy. This study provides the statistical evidence for promoting the equity of allocating health resources and facilitates the government to make corresponding policies to reasonably plan health resources.

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    Review
    Research advances in transcription regulation related to azole resistance of Candida albicans
    LIU Jin-yan, SHI Ce, WANG Ying, et al
    2016, 36 (02):  291. 
    doi: 10.3969/j.issn.1674-8115.2016.02.027

    Abstract ( 583 )   PDF (756KB) ( 725 )  

    In recent 30 years, Candida albicans has become the most common opportunistic pathogen that causes the incidence and mortality of patients with low immunity. With the frequent use of azole antifungal drugs, azole-resistant strains are often detected. Studies on the mechanism of drug resistance have drawn more and more attention. In recent years, studies on the mechanism of transcriptional regulation have also made great progress. The cis-acting elements and trans-acting factors of the resistant-related encoding gene were identified and the acting mechanism was partially elucidated. In this paper, research advances in the transcription regulation related to azole resistance of Candida albicans are reviewed.

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    Research progresses of clinical prediction of development of IgA nephropathy towards end-stage renal disease
    ZHOU Yue-ling, JIANG Geng-ru
    2016, 36 (02):  296. 
    doi: 10.3969/j.issn.1674-8115.2016.02.028

    Abstract ( 934 )   PDF (738KB) ( 841 )  

    IgA nephropathy (IgAN) is the most common primary glomerulonephritis in the world with a highly variable course. About 15% to 40% of patients will progressively develop to end-stage renal disease (ESRD) within 10 to 20 years after diagnosis. Many studies indicate that the incidence of IgAN in Asia is higher than that in North America and Europe. Asian patients are more likely to develop to ESRD and IgAN has become the principal primary glomerulonephritis leading to ESRD in Asian population. Risk factors that cause the incidence of ESRD of patients with IgAN include proteinuria, decline of estimated glomerular filtration rate, severe histopathological injury, hypertension, hypoalbuminemia, hyperuricemia, and anemia. Other factors such as male gender, hematuria, and unhealthy lifestyles are controversial. Meanwhile, researchers have established various models for predicting the risk of this disease to develop to ESRD, including Goto model, Utsunomiya model, Berthoux model, and Ruijin Hospital model, etc, which provide the possibility for early prediction and intervention.

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    Research advances in protective effect of resveratrol on intestine
    GAO Run-ying, CAI Mei-qin
    2016, 36 (02):  302. 
    doi: 10.3969/j.issn.1674-8115.2016.02.029

    Abstract ( 926 )   PDF (743KB) ( 870 )  

    Intestine has the physiological function of digestion and absorption, as well as barrier function of blocking exogenous harmful substances from human body. It also plays a very important role in maintaining body functions. As a physiological active substance, resveratrol can alleviate bowel dysfunction and protect impaired intestine through anti-inflammation, anti-fibrosis, anti-oxidation, and inhibition of apoptosis, etc. In this article, the research advances in the protective effect of resveratrol on intestinal function are reviewed, which can provide the scientific basis for the possible use of resveratrol as a dietary supplement or a medicine for intestinal diseases.

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    Predictive value of 18F-FDG PET and International Prognostic Index for DLBCL patients treated by R-CHOP and the developing trend
    LI Xue-jing, HUANG Gang
    2016, 36 (02):  307. 
    doi: 10.3969/j.issn.1674-8115.2016.02.030

    Abstract ( 642 )   PDF (820KB) ( 724 )  

    The R-CHOP combined chemotherapy has largely improved the curability of patients with CD 20+ diffuse large B cell lymphoma (DLBCL). As classic methods for predicting the prognosis, now 18F-FDG PET and international prognostic index (IPI) is facing the challenge of rituximab. This paper summarizes the predictive efficiency and research advances of two methods. For PET, the assessment has been changes from traditional vision assessment to quantitative/semi-quantitative parameter assessment. For IPI, ages and LDH levels are refined and grouped. After comparison, it is recommended that two methods should be integrated in order to better predict the development of the disease.

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