上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

青藤碱衍生物4EDB的合成、鉴定以及对实验性自身免疫性心肌炎的治疗作用观察

苏晓莲 1，吕宏祥 1，姜媛媛 1，周姗姗 1，唐晴 2，苏兆亮 1，邵晓轶 1,2   

  1. 1. 江苏大学免疫研究所，镇江 212013；2. 南通大学医学院免疫学系，南通 226001
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 邵晓轶，电子信箱：xy_shao@163.com。
  • 作者简介:苏晓莲（1989—），女，硕士生；电子信箱：sxl15262902499@163.com。
  • 基金资助:

    国家自然科学基金（81370084）；江苏省普通高校研究生实践创新计划项目（省立校助SJLX15_0518）；南通市科技计划项目（MS12016007）

Synthesis and identification of sinomenine derivate 4EDB and its therapeutic effects on mice with experimental autoimmune myocarditis

SU Xiao-lian1, Lü Hong-xiang1, JIANG Yuan-yuan1, ZHOU Shan-shan1, TANG Qing2, SU Zhao-liang1, SHAO Xiao-yi1, 2   

  1. 1. Institute of Immunology, Jiangsu University, Zhenjiang 212013, China; 2. Department of Immunology, Medical School, Nantong University, Nantong 226001, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    National Natural Science Foundation of China, 81370084; Practice Innovation Program of Ordinary University Graduate Students in Jiangsu Province, SJLX15_0518; Project of Nantong Municipal Science and Technology Bureau, MS12016007

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摘要：

目的 ·制备青藤碱衍生物4EDB，研究体内4EDB对实验性自身免疫性心肌炎（EAM）小鼠的治疗作用。方法 ·采用随机分组方法，将BALB/c小鼠分为空白对照组、EAM+4EDB组和EAM组，每组6只。免疫开始的第14日开始灌胃给药，5 mg/（kg·d）。第21日处死小鼠，取出心脏做病理切片，并进行天狼星红染色和苏木精-伊红染色（H-E染色）；real-time PCR检测心肌组织相关炎症因子白介素-1（IL-1）、IL-6、IL-17A、转化生长因子-β(TGF-β)的表达。结果 ·与EAM组相比，
EAM+4EDB组心肌炎症病理评分降低（P=0.020），纤维化得到缓解，心肌组织炎症因子IL-1、IL-6、IL-17A、TGF-β水平下降（P<0.05）。结论 ·新合成的青藤碱衍生物4EDB对EAM模型小鼠的心肌炎症有缓解作用，其机制可能与其抑制炎症因子表达有关。

关键词: 青藤碱, 4EDB, 实验性自身免疫性心肌炎, 炎症

Abstract:

Objective · To synthesize 4EDB, the derivative of sinomenine, and to observe its therapeutic effects on mice with experimental autoimmune myocarditis (EAM). Methods · The BALB/c mice were randomly divided into three groups: control group, EAM group and EAM+4EDB group with 6 mice in each group, and then treated with 4EDB [5 mg/(kg·d)] by intragastric administration from the 14th day of immunization until sacrificed on the 21th day. Hematoxylin-eosin (H-E) staining was used to identify the areas of inflammation, and the hematoxylin-picrosirius red staining was used to detect the degree of myocardial fibrosis. The expression levels of related inflammatory factors including IL-1, IL-6, IL-17A and TGF-β in myocardial tissues were detected by real-time PCR. Results · Compared with EAM group, the pathological score of myocardial inflammation decreased in EAM+4EDB group (P=0.020), with myocardial fibrosis alleviated, and the expression levels of inflammatory cytokines including IL-1, IL-6, IL-17A and TGF-β in myocardium reduced (P<0.05). Conclusion · The new derivative of sinomenine, 4EDB, can alleviate the myocarditis in EAM mice, and the inhibition of inflammatory factors may play an important role.

Key words: sinomenine, 4EDB, experimental autoimmune myocarditis, inflammation