上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (10): 1169-.

• 论著（基础研究） • 上一篇    下一篇

内毒素休克大鼠肾组织TLR2 mRNA和TLR4 mRNA的表达

张丽梅1, 张育才2, 黄绮薇1, 龚小慧2   

  1. 上海交通大学 儿童医院 1. 新生儿科, 2. 重症监护病房, 上海 200040
  • 出版日期:2009-10-25 发布日期:2009-10-26
  • 通讯作者: 张育才, 电子信箱: zyucai2005@hotmail.com。
  • 作者简介:张丽梅（1981—）, 女, 硕士生；电子信箱: zlmmay1281022@126.com。
  • 基金资助:

    上海市卫生局基金（05041）

Expression of TLR2 mRNA and TLR4 mRNA in renal tissues of rats with endotoxin-induced shock

ZHANG Li-mei1, ZHANG Yu-cai2, HUANG Qi-wei1, GONG Xiao-hui2   

  1. 1. Neonatal Department, 2. Critical Care Unit, Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China
  • Online:2009-10-25 Published:2009-10-26
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 05041

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摘要：

目的 探讨内毒素休克大鼠肾组织Toll样受体2（TLR2） mRNA和TLR4 mRNA的表达变化，以及血管活性肠肽（VIP）和甲基泼尼松龙（MP）的作用及可能机制。方法 采用大肠杆菌脂多糖（LPS）（E coli O55:B5）10 mg/kg静脉注射，建立大鼠内毒素休克模型(LPS组，n=16)；LPS+VIP组(n=16)建模后注射VIP（5 nmol/kg），LPS+VIP+MP组（n=16）注射VIP（5 nmol/kg）和MP（3 mg/kg）；另设生理盐水对照组（n=8）。透射电子显微镜观察LPS注射6 h和24 h大鼠肾组织病理学变化；RT-PCR测定肾组织TLR2 mRNA和TLR4 mRNA的表达。结果 建模后6 h，电子显微镜下见LPS组肾小球毛细血管内皮细胞肿胀，线粒体空泡变性；建模后24 h，可见LPS组肾小管细胞线粒体肿胀、空泡变性，细胞核轻度固缩；LPS+VIP组及LPS+VIP+MP组肾组织病变轻于同期LPS组。LPS注射6 h，LPS组、LPS+VIP组及LPS+VIP+MP组肾组织TLR2 mRNA和TLR4 mRNA表达均高于对照组（P<0.01）；LPS注射24 h，LPS组TLR2 mRNA和TLR4 mRNA表达高于其他组（P<0.01）。结论 内毒素休克大鼠肾组织TLR2 mRNA和TLR4 mRNA表达增强；VIP和MP干预可减轻肾脏损伤，可能与其下调TLR2 mRNA和TLR4 mRNA的表达有关。

关键词: 内毒素, 休克, Toll样受体, 血管活性肠肽, 甲基泼尼松龙, 肾脏损伤, 大鼠

Abstract:

Objective To investigate the expression of Toll like receptor 2 (TLR2) mRNA and TLR4 mRNA in renal tissues of rats with endotoxin-induced shock, and explore the possible effects and mechanism of vasoactive intestinal peptide (VIP) and methylprednisolone (MP) on renal injury. Methods Endotoxin (E coli O55:B5 LPS, 10 mg/kg) was injected intravenously to the rats to establish the endotoxin-induced shock models (LPS group, n=16). The rats in LPS+VIP group (n=16) was injected with VIP (5 nmol/kg) after model establishment, and those in LPS+VIP+MP group (n=16) was injected with VIP (5 nmol/kg) and MP (3 mg/kg) after model establishment. Besides, normal saline control group (control group) was established(n=8). Electron microscope was used to observe the histopathological changes in renal tissues 6 h and 24 h after model establishment, and RT-PCR was performed to evaluate the expression of TLR2 mRNA and TLR4 mRNA in renal tissues. Results Six h after model establishment, glomerular endothelial cell swelling and vacuolar degeneration of mitochondria were observed in LPS group under electronic microscope. Twenty-four h after model establishment, mitochondrial swelling, vacuolar degeneration and slight nucleus pyknosis of renal tubular cells were observed in LPS group. The renal pathological changes in LPS+VIP group and LPS+VIP+MP group were much less severe than those in LPS group at the same time. The expression of TLR2 mRNA and TLR4 mRNA in LPS, LPS+VIP and LPS+VIP+MP groups was significantly higher than that in control group 6 h after LPS injection (P<0.01), while the expression of TLR2 mRNA and TLR4 mRNA in LPS group was higher than that in the other groups 24 h after LPS injection (P<0.01). Conclusion The expression of TLR2 mRNA and TLR4 mRNA in renal tissues of rats with endotoxin-induced shock is markedly up-regulated. VIP and MP can obviously alleviate renal pathological changes, and the mechanism may contribute to impacting on the expression of TLR2 mRNA and TLR4 mRNA.

Key words: endotoxin, shock, Toll like receptor, vasoactive intestine peptide, methylprednisolone, kidney injury, rat

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