上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (12): 1496-.doi: 10.3969/j.issn.1674-8115.2010.12.012

• 论著（基础研究） • 上一篇    下一篇

国产13C-辛酸呼气试验评估小鼠胃排空的实验研究

张 敏1, 管 樑1, 李 彪1, 朱承谟1, 尹红燕1, 郭 睿1, 卢伟京2, 侯秀红2, 卢 浩2, 李良君2, 杜晓宁2   

  1. 1.上海交通大学 |医学院附属瑞金医院核医学科, 上海 200025；2.上海化工研究院, 上海 200062
  • 出版日期:2010-12-25 发布日期:2010-12-31
  • 通讯作者: 管 樑, 电子信箱: lanege2222@yahoo.com。
  • 作者简介:张 敏（1981—）, 男, 住院医师, 硕士；电子信箱: zmjacques@hotmail.com。
  • 基金资助:

    国家高技术研究发展计划（“八六三”计划）（2008AA02Z427）；上海市重点学科建设项目（S30203）

Mouse gastric emptying assessment by domestic 13C-octanoic acid breath test system

ZHANG Min1, GUAN Liang1, LI Biao1, ZHU Cheng-mo1, YIN Hong-yan1, GUO Rui1, LU Wei-jing2, HOU Xiu-hong2, LU Hao2, LI Liang-jun2, DU Xiao-ning2   

  1. 1.Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；2.Shanghai Research Institute of Chemical Industry, Shanghai 200062, China
  • Online:2010-12-25 Published:2010-12-31
  • Supported by:

    Hi-Tech Research and Development Program of China,“863” Program, 2008AA02Z427；Shanghai Leading Academic Discipline Project, S30203

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摘要：

目的 通过建立国产13C-辛酸呼气试验胃排空检测系统，评估糖尿病小鼠胃排空异常以及胃动力药物对胃动力功能的影响。方法 13C-辛酸采用格利雅方法合成标记。四氧嘧啶尾静脉注射制作糖尿病小鼠模型。多时间点（0、5、10、15、20、25、30、45、60、75、90、105、120 min）采集小鼠13C-辛酸灌胃后呼出的气样，红外线同位素能谱仪测定气样并获得参数13CO2峰值（Cmax）与半排空时间（T1/2）。分析13C-辛酸剂量对胃排空参数的影响及试验的可重复性，评估胃动力药物对糖尿病小鼠胃排空的干预作用，以及13C-辛酸的异常毒性作用。结果 13C-辛酸剂量与Cmax呈正相关（r= 0.954，P<0.05），但对T1/2无显著影响。重复呼气试验的T1/2变异系数为（4.76±1.43）%。正常对照组和糖尿病组小鼠的T1/2分别为（28.2±6.8）min和（28.1±4.8）min，差异无统计学意义（P>0.05）；格瑞林使糖尿病小鼠T1/2显著缩短为（19.6±4.9）min（P<0.05），而硫酸阿托品则使T1/2明显延长至（40.7±7.0）min（P<0.05）。13C-辛酸异常毒性试验未见明显毒副反应。结论 该研究所建立的小鼠13C-辛酸呼气试验胃排空检测系统具有非侵入性及良好的可重复性，能够有效检测胃排空异常和胃动力药物对胃排空的影响。

关键词: 13C-辛酸, 呼气试验, 胃排空, 小鼠

Abstract:

Objective To establish a domestic 13C-octanoic acid breath test system for gastric emptying assessment, evaluate the abnomal gastric emptying and investigate the effect of gastroprokinetic agents on gastric empting in diabetic mice. Methods 13C-octanoic acid was synthesized with Grignard method. Diabetic mouse models were established by intravenous injection of alloxan tetrahydrate. After gastric irrigation with 13C-octanoic acid to mice, expired air samples were collected at various time points (0, 5, 10, 15, 20, 25, 30, 45, 60, 75, 90, 105 and 120 min）and were examined with an infra red isotope analyzer for maximum concentration (Cmax) and half emptying time (T1/2) of 13CO2. Effect of 13C-octanoic acid dosage on gastric emptying parameters and test reproducibility were analyzed. The effect of gastroprokinetic agent intervention on gastric empting of diabetic mice was evaluated, and the abnormal toxicity of 13C-octanoic acid was observed. Results 13C-octanoic acid dosage was positively related to Cmax (r=0.954，P<0.05), while had no significant effect on T1/2. The coefficient of variation for reproducibility was (4.76±1.43)%. There was no significant difference in T1/2 between normal group and diabetes group [(28.2±6.8) min vs (28.1±4.8) min, P>0.05]. Ghrelin significantly decreased T1/2 to (19.6±4.9) min （P<0.05）, while atropine significantly increased T1/2 to (40.7±7.0) min (P<0.05) in diabetic mice. No apparent toxicity was observed in 13C-octanoic acid abnormal toxicity test. Conclusion The established 13C-octanoic acid breath test system can evaluate the gastric emptying and determine the effect of gastroprokinetic agents on gastric empting in mice, with no invasion and favourable reproducibly.

Key words: 13C-octanoic acid, breath test, gastric emptying, mouse