上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 129-.

• 专题报道（儿科学研究） • 上一篇    下一篇

RANTES和Eotaxin-3基因单核苷酸多态性与儿童哮喘的关系

霍 婧, 刘全华, 华 丽, 鲍一笑   

  1. 上海交通大学 医学院新华医院儿内科, 上海 200092
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 鲍一笑, 电子信箱: dr.smilebao@yahoo.com.cn。
  • 作者简介:霍 婧（1984—）, 女, 硕士生；电子信箱: huojing0202@163.com。
  • 基金资助:

    国家自然科学基金（30872805）和上海市科学技术委员会科研计划项目（08411953200）

Association of single nucleotide polymorphisms in RANTES and Eotaxin-3 genes with childhood asthma

HUO Jing, LIU Quan-hua, HUA Li, BAO Yi-xiao   

  1. Department of Pediatrics, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    National Natural Science Foundation of China, 30872805；Shanghai Science and Technology Committee Foundation, 08411953200

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摘要：

目的 研究趋化因子激活调节正常T细胞表达和分泌因子（RANTES）基因C-28G（RANTES C-28G）、RANTES A-403G及嗜酸性粒细胞趋化蛋白（Eotaxin-3）基因C+77T （Eotaxin-3 C+77T）的单核苷酸多态性（SNP）与汉族儿童哮喘的关系。方法 采集192例3~12岁汉族哮喘患儿（哮喘组）的口腔颊黏膜拭子，应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对RANTES C-28G、RANTES A-403G和Eotaxin-3 C+77T的SNP位点进行检测，以与哮喘组患儿无血缘关系的192名健康志愿者（18～22岁）作为正常对照组。分析两组基因型及等位基因频率分布情况。结果 两组间RANTES C-28G和RANTES A-403G两个SNP位点的基因型分布比较，差异无统计学意义（P>0.05）。两组间Eotaxin-3 C+77T基因型分布比较，差异有统计学意义；哮喘组Eotaxin-3 C+77T T/T纯合子基因型频率为32.3%，明显高于正常对照组的12.5%（OR=3.44，P=0.000）。结论 Eotaxin-3 C+77T可能是汉族儿童哮喘易感SNP位点，其中Eotaxin-3 C+77T T/T纯合子基因型与哮喘发病密切相关。

关键词: 激活调节正常T细胞表达和分泌因子, 嗜酸性粒细胞趋化蛋白, 单核苷酸多态性, 哮喘, 儿童, 汉族

Abstract:

Objective To investigate the association of single nucleotide polymorphisms (SNPs) in regulated upon activation normal T cell expressed and secreted (RANTES) gene C-28G (RANTES C-28G), RANTES A-403G and Eotaxin-3 gene C+77T (Eotaxin-3 C+77T) with asthma in Han ethnic children. Methods The buccal mucosa swabs of 192 Han ethnic children with asthma (asthma group) were collected,  and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect the SNP loci of RANTES A-403G, C-28G and Eotaxin-3 C+77T. Besides, another 192 healthy subjects (aged 18 to 22 years) without sibship with those in asthma group were served as controls. Genotype and genotypic distribution between these two groups were analysed. Results There was no significant differences in genotype and genotypic distribution of SNP loci of RANTES A-403G and RANTES C-28G between asthma group and control group (P>0.05), while there were significant differences in genotypic distribution of Eotaxin-3 C+77T between these two groups. The frequency of Eotaxin-3 C+77T T/T genotype in asthma group was significantly higher than that in control group (32.3% vs 12.5%, OR=3.44, P=0.000). Conclusion Eotaxin-3 C+77T may be the asthma susceptible SNP loci for Han ethnic children, and Eotaxin-3 C+77T T/T is significantly related with the development of childhood asthma.

Key words: regulated upon activation normal T cell expressed and secreted, Eotaxin, single nucleotide polymorphisms, asthma, children, Han ethnic