上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 213-.

• 论著（临床研究） • 上一篇    下一篇

胃癌组织中CD133 mRNA表达及与临床病理特征的关系

张 鹏1, 吴巨钢1, 伍宏彪1, 王世婷2, 俞继卫1, 姜波健1   

  1. 上海交通大学 医学院第三人民医院 1. 普外科, 2. 实验中心, 上海 201900
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 姜波健, 电子信箱: Jiang_md@hotmail.com。
  • 作者简介:张 鹏（1983—）, 男, 硕士生；电子信箱: doc.vh@hotmail.com。
  • 基金资助:

    上海交通大学医学院科研项目（2007XJ032, 2009XJ21037）；上海市科委科研项目（09411962300）

Expression of CD133 mRNA in tissues of gastric cancer and its relationship with clinicopathological features

ZHANG Peng1, WU Ju-gang1, WU Hong-biao1, WANG Shi-ting2, YU Ji-wei1, JIANG Bo-jian1   

  1. 1. Department of General Surgery, 2. Laboratory Center, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    Shanghai Jiaotong University School of Medicine Foundation, 2007XJ032, 2009XJ21037；Shanghai Science and Technology Committee Foundation, 09411962300

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摘要：

目的 探讨CD133 mRNA在胃癌组织中的表达及与临床病理特征的关系。方法 选取31例手术切除的胃癌组织和癌旁正常胃黏膜组织标本，半定量RT-PCR检测CD133 mRNA的表达，分析胃癌组织CD133 mRNA表达与性别、年龄、肿瘤直径、浸润深度、TNM分期、肿瘤分化程度、淋巴结转移和Ki-67增殖指数等临床病理特征的关系。结果 胃癌组织和癌旁正常胃黏膜组织CD133 mRNA的相对灰度值分别为0.378 3±0.141 1和0.038 1±0.091 9 (P=0.000）；肿瘤直径>5 cm者胃癌组织CD133 mRNA相对灰度值显著高于肿瘤直径≤5 cm者[(0.439 3±0.148 4) vs (0.334 3±0.121 2)](P=0.041)；有淋巴结转移者显著高于无淋巴结转移者[(0.426 6±0.132 0) vs (0.239 5±0.030 9)]（P=0.004)，淋巴结转移率和转移淋巴结数与CD133 mRNA相对灰度值呈正相关（r=0.466，P=0.008； r=0.464，P=0.009）；Ki-67低表达者CD133 mRNA相对灰度值显著高于Ki-67高表达者[(0.436 4±0.139 8) vs (0.316 4±0.117 4)]（P=0.02)，Ki-67表达与CD133 mRNA相对灰度值呈负相关(r=－0.461，P=0.009）。结论 胃癌组织CD133 mRNA的表达与反映淋巴转移的淋巴结转移率和转移淋巴结数及反映肿瘤增殖状态的Ki-67表达相关。

关键词: 胃, CD133 mRNA, 淋巴转移, 新生物/癌

Abstract:

Objective To investigate the expression of CD133 mRNA in tissues of gastric cancer, and explore its relationship with clinicopathological features. Methods The tissues of gastric cancer and normal tissues adjacent to gastric cancer were obtained from 31 patients. The expression of CD133 mRNA was detected by semi-quantitative RT-PCR, and its relationship with clinicopathological features such as sex, age, tumor diameter, infiltration depth, TNM staging, tumor differentiation, lymph node metastasis and Ki-67 proliferation index was analysed. Results The relative gray scale values of CD133 mRNA in tissues of gastric cancer and normal tissues adjacent to gastric cancer were 0.378 3±0.141 1 and 0.038 1±0.091 9, respectively (P=0.000). The relative gray scale values of CD133 mRNA in tissues of gastric cancer with tumor diameter ＞5 cm were significantly higher than those with tumor diameter ≤5 cm [(0.439 3±0.148 4) vs (0.334 3±0.121 2)](P=0.041), and those in tissues with lymph node metastasis were significantly higher than those without lymph node metastasis [(0.426 6±0.132 0) vs (0.239 5±0.030 9)]（P=0.004). The rate of lymph node metastasis and the number of metastatic lymph nodes were positively related to relative gray scale values of CD133 mRNA（r=0.466, P=0.008; r=0.464, P=0.009）. The relative gray scale values of CD133 mRNA in those with low expression of Ki-67 were significantly higher than those with high expression of Ki-67 [(0.436 4±0.139 8) vs (0.316 4±0.117 4)]（P=0.02), and expression of Ki-67 were negatively related to relative gray scale values of CD133 mRNA (r=－0.461, P=0.009). Conclusion The expression of CD133 mRNA in tissues of gastric cancer was associated with the rate of lymph node metastasis, number of metastatic lymph nodes and expression of Ki-67, which reflect the status of lymph node metastasis and proliferation of gastric cancer.

Key words: stomach, CD133 mRNA, lymphatic metastasis, neoplasma/cancer