上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (3): 318-.

• 论著（基础研究） • 上一篇    下一篇

丙酮酸乙酯对小鼠缺血再灌注肾脏细胞凋亡的影响

徐欣晖1, 陈 怡1, 张星宇1, 吕利雄1, 朱长清1, 戴慧丽2, 钱家麒2   

  1. 上海交通大学 医学院仁济医院 1. 急诊科, 2. 肾脏科, 上海 200127
  • 出版日期:2010-03-25 发布日期:2010-03-24
  • 作者简介:徐欣晖（1972—）, 男, 副主任医师, 博士；电子信箱: xinhui_72@hotmail.com。

Effects of ethyl pyruvate on apoptosis of renal cells with ischemia-reperfusion in mice

XU Xin-hui1, CHEN Yi1, ZHANG Xing-yu1, LV Li-xiong1, ZHU Chang-qing1, DAI Hui-li2, QIAN Jia-qi2   

  1. 1. Department of Emergency, 2. Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
  • Online:2010-03-25 Published:2010-03-24

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摘要：

目的 研究丙酮酸乙酯（EP）对小鼠缺血再灌注（I/R）肾脏组织凋亡相关基因表达及细胞凋亡的影响。方法 8周龄雄性BABL/c小鼠52只，随机分为假手术组（n=10）、I/R组（建立I/R模型，n=10）、EP处理组（n=32）；EP处理组再分为EP预处理组（建模前30 min给药）和I/R后4、6、12 h EP处理组（分别于建模后4、6、12 h给药），每组8只动物。TUNEL染色分析和比较各组凋亡细胞数量的变化；Real-time PCR检测肾脏组织凋亡相关基因Bcl-2、Bax、Caspas-3 mRNA表达。结果 TUNEL染色分析显示，与假手术组比较，I/R组凋亡细胞数量明显增多（P<0.05）；EP预处理组凋亡细胞数量明显少于I/R组（P<0.05）。Real-time PCR检测结果显示，与假手术组相比，I/R组Bax和Caspase-3 mRNA表达显著升高，Bcl-2 mRNA表达明显降低（P<0.05）；与I/R组比较，EP预处理组和I/R后6 h EP处理组的Bcl-2 mRNA表达显著升高（P<0.05），EP预处理组和I/R后各时间点EP处理组的Bax和Caspase-3 mRNA表达明显降低（P<0.05）。结论 EP能有效抑制BABL/c小鼠肾脏I/R过程中的细胞凋亡，可能与其调控肾脏组织凋亡相关基因的表达有关。

关键词: 丙酮酸乙酯, 缺血再灌注, 细胞凋亡, 凋亡相关基因, 肾脏

Abstract:

Objective To investigate the effects of ethyl pyruvate (EP) on expression of apoptosis related gene of renal tissues and apoptosis of renal cells with ischemia-reperfusion (I/R) in mice. Methods Fifty-two male BABL/c mice of 8 weeks old were randomly divided into sham operation group (n=10), I/R group (I/R model was established, n=10) and EP treatment group (n=32). EP treatment group was subdivided into EP pretreatment group (administration of EP 30 min before model establishment, n=8) and 4 h, 6 h and 12 h post-I/R EP treatment groups (administration of EP 4 h, 6 h and 12 h after model establishment, respectively, n=8 in each group). Cell apoptosis of each group was analysed by TUNEL staining, and the expression of apoptosis related gene Bcl-2, Bax and Caspase-3 mRNA was detected by Real-time PCR. Results TUNEL staining analysis indicated that the number of apoptotic cells in I/R group was much larger than that in sham operation group (P<0.05), and the number of apoptotic cells in EP pretreatment group was much smaller than that in I/R group (P<0.05). Real-time PCR revealed that the expression of Bax and Caspase-3 mRNA was significantly higher and the expression of Bcl-2 mRNA was significantly lower in I/R group than that in sham operation group (P<0.05), the expression of Bcl-2 mRNA was significantly higher in EP pretreatment group and 6 h post-I/R EP treatment group than that in I/R group (P<0.05), and the expression of Bax and Caspase-3 mRNA was significantly lower in EP pretreatment group and 4 h, 6 h and 12 h post-I/R EP treatment groups than that in I/R group (P<0.05). Conclusion EP can effectively inhibit apoptosis of renal cells with I/R in BABL/c mice, which may be related to the regulation of apoptosis related gene of renal tissues.

Key words: ethyl pyruvate, ischemia-reperfusion, apoptosis, apoptosis related gene, kidney