›› 2011, Vol. 31 ›› Issue (11): 1564-.doi: 10.3969/j.issn.1674-8115.2011.11.013

• 论著(基础研究) • 上一篇    下一篇

葛根总黄酮联合三氧化二砷对Kasumi-1和HL-60细胞增殖和凋亡的影响

唐宇宏1, 邵化敏2, 朱红青3, 姜鹏君4, 季建敏4, 沈 群4   

  1. 1.上海交通大学 医学院附属第三人民医院血液科, 上海201900; 2.中国人民解放军第155医院血液肿瘤科, 开封 475003; 3.马鞍山市中心医院血液科, 马鞍山 243000; 4.南京中医药大学附属医院血液科, 南京 210009
  • 出版日期:2011-11-28 发布日期:2011-11-29
  • 通讯作者: 沈 群, 电子信箱: shenq@medmail.com.cn。
  • 作者简介:唐宇宏(1978—), 男, 住院医师, 博士;电子信箱: yuhong_tang@126.com。
  • 基金资助:

    江苏省卫生厅“科教兴卫工程”医学重点人才基金(RC2007068);江苏省“六大人才高峰”第五批高层次人才基金(2008年)

Effects of co-treatment with puerariae radix flavones and arsenic trioxide on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells

TANG Yu-hong1, SHAO Hua-min2, ZHU Hong-qing3, JIANG Peng-jun4, JI Jian-min4, SHEN Qun4   

  1. 1.Department of Hematology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China;2.Department of Hematology and Oncology, No.155 Hospital of People's Liberation Army, Kaifeng 475003, China;3.Department of Hematology, The Central Hospital of Maanshan, Maanshan 243000, China;4.Department of Hematology, the Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210009, China
  • Online:2011-11-28 Published:2011-11-29
  • Supported by:

    Foundation of Department of Health, Jiangsu Province, RC2007068;Talent Program of Jiangsu Province, 2008

摘要:

目的 探讨葛根总黄酮(PRF)联合三氧化二砷(ATO)对M2型急性髓系白血病细胞株Kasumi-1和HL-60细胞增殖和凋亡的影响。方法 以100 μg/mL PRF和1 μmol/L ATO联合处理Kasumi-1和HL-60细胞48 h(RRF+ATO组),MTT法检测细胞增殖抑制率,Annexin V-FITC/PI双染法流式细胞术检测细胞早期凋亡率,Real-Time PCR检测凋亡抑制基因Bcl-2、Survivin mRNA表达,Western blotting检测细胞凋亡相关蛋白酶Caspase-3、-8、-9的表达。设立单用100 μg/mL PRF的PRF处理组和空白对照组。结果 RRF+ATO组Kasum-1细胞增殖抑制率、早期凋亡率以及Caspase-3、-9表达均显著高于PRF处理组(P<0.05),细胞中Bcl-2 mRNA表达的下调趋势明显。RRF+ATO组HL-60细胞各项指标检测结果与PRF处理组比较差异均无统计学意义(P>0.05)。结论 PRF联合ATO能有效抑制Kasumi-1细胞增殖并诱导早期凋亡,而对HL-60细胞则无明显影响。

关键词: 葛根总黄酮, 三氧化二砷, 细胞增殖, 细胞凋亡, M2型急性髓系白血病

Abstract:

Objective To investigate the effects of co-treatment with puerariae radix flavones (PRF) and arsenic trioxide (ATO) on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells of acute myeloid leukemia M2. Methods Kasumi-1 cells and HL-60 cells were treated with 100 μg/mL PRF and 1 μmol/L ATO for 48 h (RRF+ATO group). MTT assay was employed to measure the inhibition rate of cell proliferation, Annexin V-FITC/PI double staining was used to determine the early apoptosis rate by flow cytometry, the expression of Bcl-2 and Survivin mRNA was detected by Real-Time PCR, and the expression of Caspase-3, Caspase-8 and Caspase-9 protein was detected by Western blotting. Cells only treated with 100 μg/mL PRF were served as PRF group, and blank control group was also established. Results The inhibition rate of cell proliferation, early apoptosis rate and expression of Caspase-3 and Caspase-9 protein of Kasumi-1 cells in RRF+ATO group were significantly higher than those in PRF group (P<0.05), and there was an obvious trend of down-regulation of expression of Bcl-2 mRNA in Kasumi-1 cells. There was no significant difference in each parameter of HL-60 cells between RRF+ATO group and PRF group (P>0.05). Conclusion Co-treatment with PRF and ATO can effectively inhibit the proliferation and induce early apoptosis of Kasumi-1 cells, while that has no significant effect on HL-60 cells.

Key words: puerariae radix flavones, arsenic trioxide, proliferation, apoptosis, acute myeloid leukemia M2