上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

脂肪组织成分对纤维化愈合的影响

原 博，郇京宁   

  1. 上海交通大学 医学院附属瑞金医院灼伤整形科， 上海 200025
  • 出版日期:2014-11-28 发布日期:2014-12-02
  • 作者简介:原 博（1982—）， 男， 住院医师， 博士; 电子信箱: hiyuanbo2002@163.com。
  • 基金资助:

    国家自然科学基金（81071566，81101433）

Effects of fractions of adipose tissue on fibrogenesis during wound healing

YUAN Bo, HUAN Jing-ning   

  1. Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2014-11-28 Published:2014-12-02
  • Supported by:

    National Natural Science Foundation of China, 81071566，81101433

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摘要：

目的 探讨脂肪组织成分对纤维化愈合的影响及其可能机制。方法 通过在同一动物个体中分别制作真皮和脂肪组织切割伤创伤模型，组织学检查明确脂肪组织纤维化愈合特征。经体外实验从脂肪组织中分离成熟脂肪细胞和血管基质，观察两种成分对真皮成纤维细胞增殖和分泌胶原能力的影响。结果 与真皮组织比较，脂肪组织纤维化愈合程度轻。成熟脂肪细胞对真皮成纤维细胞具有促进凋亡或坏死的作用，而脂肪组织中血管基质成分对真皮成纤维细胞分泌Ⅰ型胶原功能具有促进作用。结论 脂肪组织中的成熟脂肪细胞及血管基质成分可对成纤维细胞增殖和分泌胶原等功能产生影响，这可能是创伤后脂肪组织纤维化愈合特征的细胞学机制之一。

关键词: 脂肪组织, 纤维化, 成熟脂肪细胞, 血管基质成分, 成纤维细胞

Abstract:

Objective To explore the effects of fractions of adipose tissue on the fibrogenesis during wound healing and their possible mechanisms. Methods The models of incised wounds of the dermal tissue and adipose tissue were established in the same animal. The characteristics of fibrogenesis of the adipose tissue during wound healing were determined by the histological examination. Mature adipocytes and stromal vascular fraction (SVF) were separated from the adipose tissue in vitro and the effects of two fractions on the proliferation and collagen production of dermal fibroblasts were observed. Results Compared to the dermal tissue, the extent of fibrogenesis of the adipose tissue was lesser. Mature adipocytes promoted the necrosis or apoptosis of dermal fibroblasts and SVF of the adipose tissue promoted dermal fibroblasts to secrete type I collagen. Conclusion Mature adipocytes and SVF in the adipose tissue influence the proliferation and collagen production of fibroblasts. This may be one of cytological mechanisms that can account for the characteristics of fibrogenesis of adipose tissue after being injured.

Key words: adipose tissue, fibrogenesis, mature adipocyte, stromal vascular fraction, fibroblast