上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

柠檬酸钠对卵巢癌细胞增殖和凋亡的影响

刘 燕1，王炼炼2   

  1. 1.武警重庆总队医院妇产科, 重庆 400061； 2.重庆医科大学附属第一医院妇产科， 重庆 400042
  • 出版日期:2014-09-28 发布日期:2014-09-26
  • 通讯作者: 王炼炼, 电子信箱: 343650062@qq.com。
  • 作者简介:刘燕（1971—）， 女， 主治医师， 学士; 电子信箱: huangji6905@163.com。

Effects of sodium citrate on proliferation and apoptosis of ovarian cancer cells

LIU Yan1, WANG Lian-lian2   

  1. 1.Department of Obstetrics and Gynecology, Chongqing Corps Hospital of Chinese People's Armed Police Force，Chongqing 400061, China; 2.Department of Obstetrics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China
  • Online:2014-09-28 Published:2014-09-26

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摘要：

目的 探讨不同浓度的柠檬酸钠对卵巢癌细胞增殖和凋亡的影响。方法 卵巢癌细胞株SKOV3分为空白对照组和不同浓度柠檬酸钠处理组，分别用不同浓度（0、3、5、7、10、15 mmol/L）柠檬酸钠处理12、24、48 h，MTT比色法检测细胞增殖，计算细胞增殖抑制率和细胞克隆形成率；Western blotting检测凋亡相关蛋白聚腺苷二磷酸核糖聚合酶(PARP)和Procasease-3及自噬相关蛋白LC3和p62的表达。结果 与空白对照组比较，柠檬酸钠处理组的细胞活力和细胞克隆形成率均显著降低，并呈浓度和时间依赖性（P<0.05）。Western blotting检测结果显示：与空白对照组比较，5、10 mmol/L柠檬酸钠处理组SKOV3中PARP和Procasease-3表达增加；LC3蛋白表达上调，而p62蛋白表达下调。结论 柠檬酸钠能有效抑制卵巢癌细胞株SKOV3的增殖，并经线粒体通路诱导其凋亡，其作用随柠檬酸钠浓度的升高和作用时间的延长而增强。此外，柠檬酸钠还能诱导SKOV3细胞自噬。

关键词: 柠檬酸钠, 卵巢癌, 细胞增殖, 细胞凋亡, 自噬

Abstract:

Objective To investigate the effects of sodium citrate of different concentrations on the proliferation and apoptosis of ovarian carcinoma cells. Methods Ovarian cancer cell line SKOV3 was divided into the blank control group and sodium citrate groups, which were treated by citrate sodium of 0, 3, 5, 7, 10, and 15 mmol/L for 12, 24, and 48 h. The cell proliferation was detected by the MTT and the inhibition rate of cell proliferation and formation rate of cell clone were calculated. The expressions of apoptosis related proteins PARP and Procasease-3 and autophagy related proteins LC3 and p62 were detected by the Western blotting. Results Compared to the blank control group, the cell viability and formation rate of cell clone of the sodium citrate groups significantly decreased and were concentration and time dependent (P<0.05). The results of Western blotting showed that compared to the blank control group, the expressions of PARP and Procasease-3 in SKOV3 of the sodium citrate group treated by sodium citrate of 5 and 10 mmol/L increased; the expression of LC3 up-regulated, and the expression of p62 downregulated. Conclusion Sodium citrate can efficiently inhibit the proliferation of ovarian cancer cell line SKOV3 and induce the apoptosis via the mitochondrial pathway. The effect increases with the time and concentration of sodium citrate. Besides, sodium citrate can also induce the autophagy of SKOV3 cells.

Key words: sodium citrate, ovarian carcinoma, cell proliferation, cell apoptosis, autophagy