上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (10): 1152-.doi: 10.3969/j.issn.1674-8115.2018.10.003

• 论著·基础研究 • 上一篇    下一篇

3-甲基腺嘌呤对低氧状态下上皮性卵巢癌细胞自噬、迁移和侵袭的影响

唐中园 1, 2，张宁 2, 3，狄文 2, 3，李卫平 2, 3   

  1. 1. 上海交通大学医学院附属瑞金医院妇产科，上海 200025；2. 上海市妇科肿瘤重点实验室，上海 200127；3. 上海交通大学医学院附属仁济医院妇产科，上海 200127
  • 出版日期:2018-10-28 发布日期:2018-11-18
  • 作者简介:唐中园 (1978—)，女，主治医师，博士；电子信箱： oignonet@gmail.com。
  • 基金资助:
    国家自然科学基金（ 81101972）；2012年上海市慈善癌症研究中心慈善慢跑基金（ SCCRC12008）；上海市自然科学基金（ 16ZR1420000）

Effect of 3-methyladenine on autophagy, migration and invasion in epithelial ovarian cancer cells under hypoxia

TANG Zhong-yuan1, 2, ZHANG Ning2, 3, DI Wen2, 3, LI Wei-ping2, 3   

  1. 1. Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2. Shanghai Key Laboratory of Gynecologic Oncology, Shanghai 200127, China; 3. Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2018-10-28 Published:2018-11-18
  • Supported by:
    National Natural Science Foundation of China，81101972; Charity Jogging Fund of Shanghai ChariCancer Research Center，SCCRC12008；Natural Science Foundation of Shanghai，16ZR1420000

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摘要： 目的 ·检测 3-甲基腺嘌呤（ 3-methyladenine，3-MA）对低氧状态下上皮性卵巢癌细胞（ epithelial ovarian carcinoma，EOC）自噬、迁移和侵袭的影响。方法 ·不同浓度的 3-MA处理低氧环境下 EOC细胞， Western blotting检测自噬相关蛋白 LC3的表达，透射电子显微镜观察自噬体的生成，噻唑蓝比色法、黏附试验、 Transwell迁移试验和 Matrigel侵袭试验分别测定 EOC细胞的增殖、黏附、迁移和侵袭能力。结果 · 3-MA可以抑制低氧环境诱导的 LC3-Ⅱ表达增加以及自噬体生成。低氧环境下， 5.00 mmol/L的 3-MA作用 24 h可显著降低 EOC细胞存活率（ P0.000）；2.50 mmol/L的 3-MA作用 6 h后，细胞的黏附能力显著降低（ P0.007）； 1.25 mmol/L和 2.50 mmol/L的 3-MA均可抑制低氧环境下 EOC细胞的迁移和侵袭能力（均 P<0.01）。结论 · 3-MA在抑制低氧状态下 EOC细胞自噬的同时，具有抗细胞迁移和侵袭的作用。

关键词: 3-甲基腺嘌呤, 自噬, 上皮性卵巢癌细胞, 低氧, 迁移, 侵袭

Abstract:

Objective · To investigate the effect of 3-methyladenine (3-MA) on autophagy, migration and invasion of epithelial ovarian cancer (EOC) cells under hypoxia. Methods · Different concentrations of 3-MA were used to treat EOC cells in hypoxic environment. The of autophagy-associated protein LC3 was detectedWestern blotting. The autophagosome formation was observedtransmission electron microscopy. The proliferation, adhesion, migration and invasion of EOC cells were determinedthiazole blue colorimetry, adhesion test, Transwell migration test and Matrigel invasion test, respectively. Results · 3-MA was able to inhibit the increase of LC3-Ⅱ and autophagosome formation inducedhypoxia. In hypoxic environment, the survival rate of EOC cells was significantly decreased5.00 mmol/L 3-MA for 24 h (P0.000). The adhesion ability of EOC cells was significantly decreased2.50 mmol/L 3-MA for 6 h (P0.007). 1.25 mmol/L and 2.50 mmol/L 3-MA could inhibit the migration and invasion of EOC cells in hypoxic environment (P<0.01). Conclusion · 3-MA can inhibit the autophagy, migration and invasion of EOC cells in hypoxic environment.

Key words: 3-methyladenine (3-MA), autophagy, epithelial ovarian cancer, hypoxia, migration, invasion

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