上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (12): 1435-.doi: 10.3969/j.issn.1674-8115.2018.12.007

• 论著·基础研究 • 上一篇    下一篇

新西兰兔静脉移植内膜增生模型的构建

曹博军 1，王小文 2，邹榕江 3，吕志前 1   

  1. 1. 上海交通大学附属第六人民医院心胸外科，上海 200233；2. 重庆医科大学附属第一医院胸心外科，重庆 400016；3. 上海交通大学医学院附属仁济医院心血管外科，上海 200001
  • 出版日期:2018-12-28 发布日期:2019-01-27
  • 通讯作者: 吕志前，电子信箱：18930177678@163.com。
  • 作者简介:曹博军（1992—），男，硕士生；电子信箱： cbjsurgery@163.com。
  • 基金资助:
    上海市科学技术委员会项目 (15411952400)

Construction of the model of intimal hyperplasia in vein graft in New Zealand rabbit

CAO Bo-jun1, WANG Xiao-wen2, ZOU Rong-jiang3, Lü Zhi-qian1   

  1. 1. Department of Cardiothoracic Surgery, Shanghai Sixth People′s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China; 2. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 3. Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China
  • Online:2018-12-28 Published:2019-01-27
  • Supported by:
    Project of Science and Technology Commission of Shanghai Municipality, 15411952400

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摘要： 目的 ·构建新西兰兔自体静脉移植模型，观察移植术后不同时间段桥静脉内膜增生的变化情况。方法 ·取 10周龄雌性新西兰兔 35只，随机分成 5组，即术后 7 d组、术后 14 d组、术后 28 d组、术后 60 d组、正常对照组，每组 7只。建立颈外静脉颈总动脉旁路移植手术动物模型，并对模型构建的步骤进行优化。应用苏木精 -伊红（ hematoxylin-eosin，H-E）染色及免疫荧光染色观察静脉移植术后内膜增生情况及平滑肌细胞标志蛋白— — α平滑肌肌动蛋白 (α-smooth muscle actin，α-SMA)表达情况，评估模型构建效果。结果 ·除正常对照组外，其余 28只新西兰兔均顺利完成静脉移植手术。术后桥静脉取材发现，除术后 7 d组内有 1例发生桥静脉坏死，其余新西兰兔的桥静脉均通畅，成功率为 96.4%（27/28）。H-E染色及免疫荧光染色结果显示，与正常对照组相比，静脉移植术后各时间组桥静脉内膜厚度均明显增加（均 P0.000），α-SMA荧光表达强度均明显增强。结论 ·新西兰兔静脉移植内膜增生模型构建的成功率较高、重复性较好，其为静脉移植术后再狭窄机制的研究及防治提供了理想的实验模型。

关键词: 自体静脉移植, 内膜增生, 动物模型

Abstract:

Objective · To establish New Zealand rabbit autogenous vein graft model and observe the changes of intimal hyperplasia in vein grafts at different time after grafting. Methods · 35 female New Zealand rabbits of 10 weeks old were randomly divided into 5 groups (7 rats in each group), i.e. postoperative 7 d group, postoperative 14 d group, postoperative 28 d group, postoperative 60 d group and normal control group. An animal model of external jugular vein common carotid artery bypass grafting was established, and the model construction steps were elaborated and optimized. Hematoxylin-eosin (H-E) staining was used to observe the intimal thickness after surgery and immunofluorescence staining was used to observe the of α-smooth muscle actin (α-SMA), which was one of the vascular smooth muscle cell (VSMC) differentiation marker protein. The effect of model construction was finally evaluated. Results · In addition to the normal control group, the other 28 New Zealand rabbits were successfully completed the vein graft operation. All the grafted veins kept unblocked except for one graft in postoperative 7 d group developed thrombosis, and the success rate was 96.4% (27/28). H-E staining and immunofluorescence staining showed significant intimal hyperplasia after vein grafting. Compared with the normal control group, the intimal thickness of the vein grafts was significantly increased (all P0.000) and the fluorescence intensity of α-SMA was significantly enhanced at each time group after surgery. Conclusion · New Zealand rabbit vein graft intimal hyperplasia modeling has an ideal success rate and good repeatability, which provides an ideal experimental model for studying the mechanism and prevention of restenosis after vein grafting.

Key words: autologous vein graft, intimal hyperplasia, animal model

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