上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (4): 353-.doi: 10.3969/j.issn.1674-8115.2019.04.004

• 论著·基础研究 • 上一篇    下一篇

Fbxo22基因敲除小鼠模型的建立和表型研究

张辉林，朱晓娜，杨烁，刘朦迪，朱迪，余韵   

  1. 上海交通大学医学院附属瑞金医院分子医学中心，上海 200025
  • 出版日期:2019-04-28 发布日期:2019-05-23
  • 通讯作者: 余韵，电子信箱：yy@shsmu.edu.cn。
  • 作者简介:张辉林（1990—），女，硕士生；电子信箱： lugylucy@126.com。
  • 基金资助:
    国家自然科学基金（ 81772936）；中国福利会国际和平妇幼保健院院级科研基金（ GFY5809）

Generation and phenotypic analysis of Fbxo22knockout mice

ZHANG Hui-lin, ZHU Xiao-na, YANG Shuo, LIU Meng-di, ZHU Di, YU Yun   

  1. Center of Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-04-28 Published:2019-05-23
  • Supported by:
    National Natural Science Foundation of China, 81772936; Foundation of the International Peace Maternity & Child Health Hospital of China, GFY5809

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摘要： 目的 ·分析 Fbxo22基因敲除小鼠的表型，为探索 FBXO22的生物学功能提供理论依据。方法 ·利用 CRISPR-Cas9（clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9）技术成功构建 Fbxo22全身敲除小鼠，观察胚胎和小鼠的外观，测定其数量和质量，并分析小鼠的进食量和存活时间。结果 · Fbxo22敲除小鼠胚胎期 17.5/18.5 d的胚胎数量符合孟德尔遗传定律，外观未见异常，但是多数 Fbxo22敲除小鼠在出生后 48 h内死亡。少数存活小鼠体型偏小，进食减少，存活时间缩短。结论 · FBXO22对于小鼠出生后早期存活和正常发育有重要作用。

关键词: FBXO22, CRISPR-Cas9技术, 早期生后死亡

Abstract:

Objective · To establish the Fbxo22 knockout momodel and study the biological function of FBXO22. Methods · The Fbxo22 knockout mice were generatedCRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/-mice were analyzed. Results · Although the Fbxo22-/-embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/- mice showed reduced body size and food intake and decreased life span. Conclusion · FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development. [Key words]FBXO22; CRISPR-Cas9 technology; early postnatal lethality

Key words: FBXO22, CRISPR-Cas9 technology, early postnatal lethality

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