上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (5): 458-.doi: 10.3969/j.issn.1674-8115.2019.05.004

• 论著·基础研究 • 上一篇    下一篇

胚胎期双酚 A暴露对斑马鱼发育及神经行为的影响

潘睿 1，胡晶莹 2，户宜 3，姚谦 1，李卫华 2，田英 1, 4，高宇 1   

  1. 1.上海交通大学公共卫生学院，上海 200025；2.复旦大学生殖与发育研究院，上海市计划生育科学研究所，国家卫生健康委员会计划生育药具重点实验室，上海 200032；3.上海交通大学附属儿童医院上海市儿童医院生物医学信息研究中心，上海 200040；4.上海交通大学医学院附属新华医院环境与儿童健康重点实验室，上海 200092
  • 出版日期:2019-05-28 发布日期:2019-07-26
  • 通讯作者: 高宇，电子信箱：gaoyu_ciel@sjtu.edu.cn。
  • 作者简介:潘睿（1992—），男，硕士生；电子信箱： 18817555912@163.com。
  • 基金资助:
    国家自然科学基金（ 81872629）；上海市自然科学基金（ 17ZR1415800）；教育部和上海市环境与儿童健康重点实验室开放课题（ CEH2018072）

Effects of bisphenol A exposure during embryonic phase on development and neurobehavior of zebrafish

PAN Rui1, HU Jing-ying2, HU Yi3, YAO Qian1, LI Wei-hua2, TIAN Ying1, 4, GAO Yu1   

  1. 1. Shanghai Jiao Tong University School of Public Health, Shanghai 200025, China; 2. National Health Commission Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Reproduction and Developmental Research Institute of Fudan University, Shanghai 200032, China; 3. Center for Medical Bioinformatics, Shanghai Childrens Hospital, Shanghai Jiao Tong University, Shanghai 200040, China; 4. MOE and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2019-05-28 Published:2019-07-26
  • Supported by:
    National Natural Science Foundation of China, 81872629; Natural Science Foundation of Shanghai, 17ZR1415800; Open Subject of Ministry of Education and Shanghai Key Laboratory of Environment and Children Health, CEH2018072

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摘要： 目的 ·运用斑马鱼模型初步探讨胚胎期双酚 A（bisphenol A，BPA）暴露对斑马鱼发育和神经行为的影响。方法 ·对受精后小时数（hours post-fertilization，hpf）为 4的正常斑马鱼受精卵以不同浓度 BPA（0、2.5、25、250、2 500 μg/L）作染毒处理；观察并记录斑马鱼存活率、畸形率和孵化率，以及斑马鱼胚胎 24 hpf自主运动和 48 hpf心率，并对发育至 144 hpf的斑马鱼幼鱼进行神经行为学检测。结果 ·各浓度 BPA均未影响斑马鱼的存活率、畸形率和孵化率。暴露于 2 500 μg/L浓度 BPA的斑马鱼胚胎 24 hpf自主运动和 48 hpf心率低于对照组（ P0.000），发育至 144 hpf的幼鱼运动总距离和平均速度降低（ P0.000）。暴露于 250 μg/L和 2 500 μg/L浓度 BPA的斑马鱼幼鱼在强光刺激惊恐反射检测中运动行为改变，主要表现为在强光刺激下运动总距离减少（ P<0.05）。结论 ·胚胎期 BPA暴露对斑马鱼具有一定的发育毒性和神经毒性。

关键词: 双酚 A, 斑马鱼, 胚胎期, 发育, 神经行为

Abstract:

Objective · To investigate the effects of bisphenol A (BPA) on the development and neurobehavior of zebrafish. Methods · The zebrafish fertilized eggs that developed to 4 hpf (hours post-fertilization) were treated with different concentrations of BPA (0, 2.5, 25, 250, and 2 500 μg/L). Survival rates, malformation rates, hatching rates, 24 hpf autonomic movement, and 48 hpf heart rates in the groups were observed and recorded. And the neurobehavioral test of larval zebrafish that developed to 144 hpf was performed. Results · The BPA exposure did not affect the survival rates, malformation rates and hatching rates of zebrafish. Compared with the control group, the autonomic movement in 24 hpf and heart rate in 48 hpf of zebrafish embryos that exposed to 2 500 μg/L BPA decreased (P0.000), and the total distance and average speed of larval zebrafish that developed to 144 hpf also decreased (P0.000). The behavior of larval zebrafish exposed to 250 μg/L and 2 500 μg/L BPA changed on light-evoked startle escape response activity, mainly including the decrease of total distance under strong light stimulation (P<0.05). Conclusion · BPA exposure during embryonic phase has developmental toxicity and neurotoxicity on zebrafish.

Key words: bisphenol A, zebrafish, embryonic phase, development, neurobehavior

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