上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2020, Vol. 40 ›› Issue (1): 18-.doi: 10.3969/j.issn.1674-8115.2020.01.003

• 论著·基础研究 • 上一篇    下一篇

SIRT1基因与抑郁症及其临床表型的关联研究

赵俊雄1，华 深1，范卫星1，王卫平1，唐 伟2，张 晨1, 3   

  1. 1. 浙江省金华市第二医院，金华321016；2. 浙江省温州市康宁医院，温州 325000；3. 上海交通大学医学院附属精神卫生中心，上海 200030
  • 出版日期:2020-01-28 发布日期:2020-03-05
  • 通讯作者: 张 晨，电子信箱：zhangchen645@gmail.com。
  • 作者简介:赵俊雄（1971—），男，副主任医师，学士；电子信箱：zhaojunxiong@sohu.com。
  • 基金资助:
    国家自然科学基金（81771450）；金华市科学技术局项目（2015-3-063）；上海市科学技术委员会西医引导类项目（19411969300）。

Association of SIRT1 gene with major depression and its clinical features

ZHAO Jun-xiong, HUA Shen, FAN Wei-xing, WANG Wei-ping, TANG Wei, ZHANG Chen   

  1. 1. Jinhua Second Hospital, Zhejiang Province, Jinhua 321016, China; 2. Wenzhou Kangning Hospital, Zhejiang Province, Wenzhou 325000, China; 3. Shanghai Mental Health Center, Shanghai Jiao Tong University, School of Medicine, Shanghai 200030, China
  • Online:2020-01-28 Published:2020-03-05
  • Supported by:
    National Natural Science Foundation of China (81771450); Jinhua Science and Technology Beaureu Foundation (2015-3-063); Shanghai Science and Technology Commission Foundation (19411969300).

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摘要： 目的·探索SIRT1基因rs3758391位点与抑郁症候群之间的关系，以进一步明确SIRT1基因在抑郁症发生过程中的作用。方法·回顾性收集浙江省金华市第二医院和温州市康宁医院的重症抑郁发作患者323例，健康对照者347名，采用汉密尔顿抑郁量表（Hamilton Depression Scale，HAMD）评估患者抑郁症状，采用TaqMan探针SNP基因分型技术对rs3758391位点进行分型。采用BRAINEAS数据库分析rs3758391位点对脑内SIRT1基因mRNA表达的影响，多因素方差分析用于比较rs3758391位点3种不同基因型间症状严重程度的差异。结果·SIRT1基因rs3758391位点C、T等位基因频率比较，观察组分别为18.7%、81.3%，对照组分别为14.3%、85.7%，2组差异有统计学意义（χ24.86，P0.03）。携带rs3758391位点不同基因型患者的情绪、认知障碍和HAMD总分比较，差异有统计学意义（PSIRT1基因表达有显著性相关（P0.003）。结论·SIRT1基因rs3758391位点可能是中国汉族抑郁症的风险因子，且与抑郁症患者的疾病严重程度有关，特别是情绪症状和认知障碍。

关键词: SIRT1基因, 抑郁症, 临床维度, 汉密尔顿抑郁量表, 数量性状分析

Abstract:

Objective · To explore the relationship between rs3758391 polymorphism of SIRT1 gene and depressive symptoms, and to further understand the role of SIRT1 gene in major depressive disorder. Methods · A total of 323 patients with major depressive disorder were retrospectively collected the Jinhua Second Hospital, Wenzhou Kangning Hospital and Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine. A total of 347 healthy subjects were also recruited. Depressive symptoms were evaluatedusing the Hamilton Depression Scale (HAMD), and rs3758391 polymorphism was genotypedusing the TaqMan SNP genotyping Assay. The effect of rs3758391 polymorphism on the of SIRT1 mRNA in brain was analyzedBRAINEAS database, and the difference of depressive symptom severity among three genotypes at rs3758391 polymorphism was comparedmultivariate analysis of variance. Results · The frequencies of C and T alleles of rs3758391 polymorphism in SIRT1 gene were 18.7% and 81.3% in the case group, and 14.3% and 85.7% in the control group, respectively. The allelic distribution frequencies between the two groups were significantly different (χ24.86, P0.03). There were significant differences in mood, cognitive impairment and HAMD scores among patients with different genotypes of rs3758391 polymorphism (PSIRT1 gene in occipital cortex (OCTX) (P0.003). Conclusion · rs3758391 polymorphism of SIRT1 gene may be a risk factor for major depressive disorder in Chinese Han population, and is associated with the severity of depressive symptoms, especially with emotional symptoms and cognitive impairment.

Key words: SIRT1 gene, depression, clinical dimension, Hamilton Depression Scale, quantitative trait analysis