上海交通大学学报（医学版）

上海交通大学学报(医学版) ›› 2020, Vol. 40 ›› Issue (11): 1468-1472.doi: 10.3969/j.issn.1674-8115.2020.11.005

• 论著·基础研究 • 上一篇    下一篇

手术创伤对2型糖尿病小鼠术后认知功能和脑葡萄糖代谢的影响

罗 钢，崔永晨，曹 越，张俊峰   

  1. 上海交通大学附属第六人民医院麻醉科，上海200233
  • 出版日期:2020-11-28 发布日期:2021-01-13
  • 通讯作者: 张俊峰，电子信箱：zhangjunfeng@sjtu.edu.cn。
  • 作者简介:罗 钢（1993—），男，硕士生；电子信箱：184234041@qq.com。
  • 基金资助:
    国家自然科学基金（81571035）。

Effect of surgical trauma on postoperative cognitive function and cerebral glucose metabolism in type 2 diabetic mice

LUO Gang, CUI Yong-chen, CAO Yue, ZHANG Jun-feng   

  1. Department of Anesthesia, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2020-11-28 Published:2021-01-13
  • Supported by:
    National Natural Science Foundation of China (81571035).

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摘要： 目的·观察2型糖尿病小鼠腹部手术创伤后的学习记忆功能及相应功能脑区葡萄糖代谢功能的变化。方法·将40只雄性5周龄C57BL/6野生型小鼠用60%高脂饲料喂养，4周后使用烟酰胺和链脲佐菌素建成2型糖尿病模型，然后随机分为对照组和腹部手术创伤组（手术组），每组15只。旷场实验和条件性恐惧实验观察手术创伤对小鼠学习记忆的影响（n=10）；正电子发射型计算机断层显像（positron emission tomography/computed tomography，PET/CT）观察2组小鼠大脑相应脑区18F-氟代脱氧葡萄糖（18F-fluorodeoxyglucose，18F-FDG）的摄取水平（n=5）。结果·旷场实验中，手术组的中央格穿越次数和总移动距离与对照组差异均无统计学意义；在条件性恐惧实验中，手术组的场景相关性记忆测试和声音相关性记忆测试的僵直时间比例均显著低于对照组（均P<0.05）。PET/CT中，手术组海马、皮质、纹状体及杏仁核脑区的18F-FDG摄取率均显著高于对照组（均P<0.05）。结论·2型糖尿病小鼠腹部手术创伤后出现的认知功能障碍可能与其相关脑区葡萄糖代谢异常有关。

关键词: 术后认知功能障碍, 正电子发射型计算机断层显像, 18F-氟代脱氧葡萄糖, 脑葡萄糖代谢, 糖尿病

Abstract:

Objective · To observe the changes of learning and memory functions and as well as the changes of the glucose metabolism in the corresponding functional brain regions in type 2 diabetic mice after abdominal surgery. Methods · Forty 5-week-old male mice of C57BL/6 wild-type, which were fed on 60% high-fat diet, and the type 2 diabetes models were established with nicotinamide and streptozocin 4 weeks later. These mice were then randomly divided into control group (n=15) and abdominal surgery group (surgery group, n=15). The open field experiment and the fear conditioning experiment were performed to assess the learning and memory functions in each group (n=10). Positron emission tomography/computed tomography (PET/CT) was used to measure the uptake of 18F-fluorodeoxyglucose (18F-FDG) in different brain regions of mice in each group (n=5). Results · In the open field experiment, there was no significant difference in the number of central grid crossings and total movement distance between the control group and the surgery group. In the fear conditioning experiment, the freezing time ratios of the surgery group in the contextual test and the tone-related test were lower than those in the control group (both P<0.05). The standard intake rates of 18F-FDG in hippocampus, cerebral cortex, striatum and amygdala in the surgery group were significantly higher than those of the control group (all P<0.05). Conclusion · Postoperative cognitive dysfunction after abdominal surgery in type 2 diabetic mice may be associated with the abnormal cerebral glucose metabolism in relevant cerebral areas.

Key words: postoperative cognitive dysfunction (PCOD), positron emission tomography/computed tomography (PET/CT), 18F-fluorodeoxyglucose (18F-FDG), cerebral glucose metabolism, diabetes

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