上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (9): 1101-.doi: 10.3969/j.issn.1674-8115.2010.09.020

• 论著（基础研究） • 上一篇    下一篇

静脉高压对微血管床病理变化的实验研究

齐砚庆, 张 岚, 张纪蔚   

  1. 上海交通大学 医学院附属仁济医院血管外科, 上海 200127
  • 出版日期:2010-09-25 发布日期:2010-09-27
  • 通讯作者: 张 岚, 电子信箱: lucky200207@yahoo.com.cn；张纪蔚, 电子信箱: zhangjiwei001@sina.com.cn。
  • 作者简介:齐砚庆（1982—）, 男, 硕士生；电子信箱: qiyanqing020434@163.com。
  • 基金资助:

    上海市科委自然科学基金（10ZR1418800）

Experimental study of pathological changes of capillary vessels with venous hypertension

QI Yan-qing, ZHANG Lan, ZHANG Ji-wei   

  1. Department of Vascular Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2010-09-25 Published:2010-09-27
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 10ZR1418800

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摘要：

目的 建立适合静脉高压病理机制研究的动物模型，观察毛细血管内皮细胞Duffy抗原趋化因子受体（DARC）在静脉高压炎症反应中的作用。方法 选取SD大鼠，分别建立股动静脉瘘和髂静脉缩窄静脉高压模型，另设阴性对照和空白对照，HE染色和免疫组织化学染色法观察术后大鼠后肢肌肉及皮下组织毛细血管管腔面积的变化，并利用选出的模型观察毛细血管内皮细胞DARC表达的变化。结果 术后不同时间点股动静脉瘘模型大鼠较髂静脉缩窄模型大鼠后肢肌肉及皮下组织毛细血管管腔扩张明显且持续时间长，股动静脉瘘模型大鼠毛细血管内皮细胞DARC表达随时间延长而增加。结论 大鼠股动静脉瘘模型较适合静脉高压病理机制研究，毛细血管内皮细胞DARC的表达与静脉高压炎症反应有关。

关键词: Duffy抗原趋化因子受体, 静脉高压, 动物模型, 慢性静脉功能不全

Abstract:

Objective To establish the suitable animal model for pathological study of venous hypertension, and investigate the role of Duffy antigen receptor for chemokines (DARC) in capillary endothelial cells in the inflammatory reaction of venous hypertension. Methods SD rat femoral arteriovenous fistula venous hypertension model and iliac vein coarctation venous hypertension model were established, and negative controls and blank controls were also prepared. The changes of area of blood capillary lumens in muscle and subcutaneous tissues in hind limbs of rats were observed by HE staining and immunohistochemical staining, and the changes of expression of DARC in capillary endothelial cells were detected with selected rat models. Results The area of blood capillary lumens in muscle and subcutaneous tissues in hind limbs of rat femoral arteriovenous fistula venous hypertension model was much larger and more sustained than that of rat iliac vein coarctation venous hypertension model at different time points after model establishment. The expression of DARC in capillary endothelial cells of rat femoral arteriovenous fistula venous hypertension model increased with time after model establishment. Conclusion Rat femoral arteriovenous fistula model is more suitable for pathological study of venous hypertension, and the expression of DARC in capillary endothelial cells is related with inflammatory reaction of venous hypertension.

Key words: Duffy antigen receptor for chemokines, venous hypertension, animal model, chronic venous insufficiency