上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (3): 271-.doi: 10.3969/j.issn.1674-8115.2011.03.005

• 论著（基础研究） • 上一篇    下一篇

Pentraxin 3蛋白在兔动脉粥样硬化斑块中的表达研究

刘 华, 方唯一, 袁 方, 关韶峰, 张 敏   

  1. 上海交通大学附属胸科医院心内科, 上海 200030
  • 出版日期:2011-03-28 发布日期:2011-03-29
  • 通讯作者: 方唯一, 电子信箱: fwychest@163.com。
  • 作者简介:刘 华（1978—）, 女, 主治医师, 博士生；电子信箱: xkliuhua@126.com。
  • 基金资助:

    上海市胸科医院科技课题资助项目(Y209-11)

Expression of pentraxin 3 protein in rabbit atherosclerosis plaques

LIU Hua, FANG Wei-yi, YUAN Fang, GUAN Shao-feng, ZHANG Min   

  1. Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2011-03-28 Published:2011-03-29
  • Supported by:

    Shanghai Chest Hospital Foundation, Y209-11

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摘要：

目的 研究炎症因子正五聚素蛋白3（pentraxin 3，PTX3）在兔动脉粥样硬化斑块及正常动脉组织中的表达。方法 16只新西兰大白兔随机分为正常饮食对照组和高脂饮食组（每组8只），分别喂食普通饲料（100 g/d）和高脂饲料(100 g/d），持续16周。于实验第8周和第16周时，超声检查观察高脂饮食组主动脉粥样硬化斑块形成情况。于实验第16周，取高脂饮食组兔主动脉组织，苏丹Ⅲ染色观察主动脉粥样硬化情况。分别采用Real-Time PCR技术和Western blotting法测定高脂饮食组兔动脉粥样硬化斑块和正常饮食对照组兔正常动脉组织中PTX3 mRNA及蛋白表达。结果 实验第16周，超声检查和组织学观察均证实高脂饮食组兔主动脉有粥样硬化斑块形成；高脂饮食组动脉粥样硬化斑块中PTX3 mRNA和蛋白的相对表达量均显著高于正常饮食对照组正常动脉组织，PTX3 mRNA表达分别为（2.815±0.531）和（1.022±0.175），PTX3蛋白表达分别为（0.470±0.056）和（0.248±0.053），差异均有统计学意义（P<0.01）。结论 PTX3在动脉粥样硬化斑块中的表达明显上调，是参与动脉粥样硬化发生和发展的重要炎症因子之一。

关键词: 动脉粥样硬化斑块, 正五聚素蛋白3, 炎症因子

Abstract:

Objective To investigate the expression of pentraxin 3 (PTX3) in rabbit atherosclerosis plaques and normal artery tissues. Methods Sixteen New Zealand rabbits were randomly divided into normal diet control group (n=8) and high-fat diet group (n=8), and were fed with standard diet (100 g/d) and high-fat diet (100 g/d) for 16 weeks, respectively. At the eighth and sixteenth week of the study, the formation of atherosclerosis plaques in high-fat diet group was observed by ultrasonography. At the sixteenth week of the study, rabbit aortas of high-fat diet group were obtained, and the formation of atherosclerosis plaques was observed by sudan Ⅲ staining. The expression of PTX3 mRNA and protein in atherosclerosis plaques of high-fat diet group and normal artery tissues of normal diet control group was detected by Real-Time PCR and Western blotting, respectively. Results At the sixteenth week of the study, the formation of atherosclerosis plaques in high-fat diet group was confirmed by both ultrasonography and histological observations. The relative expression of PTX3 mRNA and protein in atherosclerosis plaques of high-fat diet group was significantly higher than that in normal artery tissues of normal diet control group [(2.815±0.531) vs (1.022±0.175) and (0.470±0.056) vs (0.248±0.053)](P<0.01). Conclusion The expression of PTX3 is significantly increased in atherosclerosis plaques, and PTX3 is one of the important inflammatory factors involved in the development of atherosclerosis plaques.

Key words: atherosclerosis plaque, pentraxin 3, inflammatory factor