上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1129-.doi: 10.3969/j.issn.1674-8115.2011.08.019

• 论著（临床研究） • 上一篇    下一篇

肿瘤相关抗原OVA66在人肿瘤组织中的表达

荣婷婷1, 饶 威1, 陈惠娟1, 王 颖1, 张 勇1, 沈立松2, 葛海良1   

  1. 1.上海交通大学 基础医学院免疫学教研室, 上海 200025； 2.上海交通大学 医学院附属新华医院检验科, 上海 200092
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 通讯作者: 葛海良, 电子信箱: gehl@shsmu.edu.cn。
  • 作者简介:荣婷婷（1984—）, 女, 硕士生；电子信箱: rtt.com@163.com。
  • 基金资助:

    国家自然科学基金（30972786）；上海市科委生物医学重点基金（10411950400）；上海市科委重点学科建设基金（JS0207）

Expression of tumor-associated antigen OVA66 in human tumor tissues

RONG Ting-ting1, RAO Wei1, CHEN Hui-juan1, WANG Ying1, ZHANG Yong1, SHEN Li-song2, GE Hai-liang1   

  1. 1.Department of Immunology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；2.Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2011-08-28 Published:2011-08-29
  • Supported by:

    National Natural Science Foundation of China,30972786；Shanghai Science and Technology Committee Foundation,10411950400；Shanghai Leading Academic Discipline Project, JS0207

PDF (PC)

1402

摘要：

目的 分析肿瘤相关抗原OVA66在人肿瘤组织中的表达特征，探讨其临床应用价值。方法 采用免疫组织化学法检测多种组织肿瘤芯片和肿瘤组织标本(包括胃癌、肠癌、肺癌、乳腺癌、膀胱癌、肾癌和卵巢癌)及其相应的非肿瘤组织中OVA66蛋白的表达，分析其表达与组织的良恶性以及肿瘤转移和临床病理特征的关系。结果 OVA66蛋白在胃、肠、卵巢、膀胱和肾的肿瘤组织中的表达显著高于正常组织，在肺和乳腺肿瘤组织中表达较弱。膀胱癌OVA66的表达与膀胱癌的病理分级呈现相关性，肠癌和膀胱癌的淋巴转移组表达阳性率略高于未转移组，但差异无统计学意义。结论 OVA66蛋白在多数肿瘤组织中均呈高表达，在膀胱癌中与肿瘤病理分级相关，提示OVA66有可能成为新的肿瘤标志物应用于多种肿瘤的辅助诊断。

关键词: OVA66, 组织芯片, 免疫组织化学

Abstract:

Objective To investigate the expression of tumor-associated antigen OVA66 in human tumor tissues, and explore its clinical values. Methods Immunohistochemistry was employed to detect the expression of OVA66 protein in several types of tumor tissue chips and samples (gastric cancer, intestinal cancer, lung cancer, breast cancer, ovarian cancer, bladder cancer and renal cancer) and corresponding non-tumor tissues. The relationship between the expression of OVA66 protein and character of tumor tissues, tumor metastasis and clinicopathological characteristics was analysed. Results There was high expression of OVA66 protein in the tumor tissues of stomach, intestine, ovary, bladder and kidney, which was significantly higher than that in non-tumor tissues. While the expression of OVA66 was relatively lower in the tumor tissues of lung and breast. The expression of OVA66 in bladder cancer tissues was related to the pathological grading of bladder cancer. The expression of OVA66 in intestinal cancer and bladder cancer with lymph node metastasis was slightly higher than that in intestinal cancer and bladder cancer without lymph node metastasis, while there was no significant difference between them. Conclusion There is high expression of OVA66 protein in many tumor tissues. The expression of OVA66 protein in bladder cancer tissues is related to the pathological grading of bladder cancer. OVA66 may serve as a novel tumor marker in the diagnosis of tumors.

Key words: OVA66, tissue microarray, immunohistochemistry