上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1125-.doi: 10.3969/j.issn.1674-8115.2011.08.018

• 论著（基础研究） • 上一篇    下一篇

补体过度激活与自身免疫型复发性流产的关系

肖世金1, 赵爱民1, 鲍世民2   

  1. 1.上海交通大学 医学院附属仁济医院妇产科, 上海 200127； 2.中国科学院上海实验动物中心, 上海 201615
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 通讯作者: 赵爱民, 电子信箱: zam0526@yahoo.com.cn。
  • 作者简介:肖世金（1983—）, 男, 硕士生；电子信箱: shjxiao@sina.com。
  • 基金资助:

    上海市创新行动计划基金（08140901500）

Relationship between excessive complement activation and autoimmune-type recurrent spontaneous abortion

XIAO Shi-jin1, ZHAO Ai-min1, BAO Shi-min2   

  1. 1.Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200217, China；2.Experimental Animal Center of Shanghai, Chinese Academy of Sciences, Shanghai 201615, China
  • Online:2011-08-28 Published:2011-08-29
  • Supported by:

    Shanghai Innovation Action Plan Foundation, 08140901500

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摘要：

目的 建立自身免疫型复发性流产(RSA)小鼠模型，探讨补体在自身免疫型RSA发病机制中的作用。方法 采用子宫注射人β2GP-1免疫Balb/c小鼠（子宫β2GP-1注射组，n=15）；分别以子宫无关蛋白注射组（n=6）、子宫佐剂注射组（n=13）、子宫生理盐水（NS）注射组（n=10）及正常未孕组（n=10)、正常妊娠组（n=15)作为对照。比较各组小鼠胚胎丢失率、胎盘平均质量、外周血补体C3浓度及胎盘组织衰变加速因子（DAF）的质量浓度。结果 子宫β2GP-1注射组小鼠胚胎丢失率显著高于正常妊娠组和各子宫注射对照组（P<0.05），胎盘平均质量显著低于正常妊娠组和各子宫注射对照组（P<0.05），外周血补体C3水平和胎盘组织DAF的质量浓度显著低于正常妊娠组和各子宫注射对照组（P<0.05）。结论 通过子宫注射人β2GP-1的方法可诱导建立符合自身免疫型RSA临床特征的小鼠模型，补体系统过度激活可能是自身免疫型RSA的发病机制之一。

关键词: 复发性流产, 自身免疫, 动物模型, β2GP-1, 补体C3, 衰变加速因子, 小鼠

Abstract:

Objective To establish the mouse model of autoimmune-type recurrent spontaneous abortion (RSA), and explore the role of complement in the pathogenesis of autoimmune-type RSA. Methods The mouse model of autoimmune-type RSA was established by intrauterine injection of human β2GP-1 in Balb/c mice (intrauterine injection of β2GP-1 group, n=15), and intrauterine injection of unrelated protein group (n=6), intrauterine injection of adjuvants group (n=13), intrauterine injection of normal saline group (n=10), normal unpregnancy group (n=10) and normal pregnancy group (n=15) were served as controls. The embryo loss rates, mean placental weight, concentrations of complement C3 in peripheral blood and mass concentrations of decay accelerating factor (DAF) in placental tissues were compared among groups. Results The embryo loss rate in intrauterine injection of β2GP-1 group was significantly higher than those in normal pregnancy group and intrauterine injection control groups （P<0.05）. The mean placental weight, concentrations of complement C3 in peripheral blood and mass concentrations of DAF in placental tissues in intrauterine injection of β2GP-1 group were significantly lower than those in normal pregnancy group and intrauterine injection control groups （P<0.05）. Conclusion Intrauterine injection of human β2GP-1 can establish autoimmune-type RSA mouse model. Excessive complement activation may paly an important role in the pathogenesis of autoimmune-type RSA.

Key words: recurrent spontaneous abortion, autoimmune, animal model, β2GP-1, complement C3, decay accelerating factor, mouse