上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1170-.doi: 10.3969/j.issn.1674-8115.2011.08.027

• 论著（临床研究） • 上一篇    下一篇

自然流产母胎界面嗜酸粒细胞趋化因子、T淋巴细胞激活上调性表达分泌因子S及其受体的表达

肖世金, 董 倩, 赵爱民   

  1. 上海交通大学 医学院附属仁济医院妇产科, 上海 200127
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 通讯作者: 赵爱民, 电子信箱: zam0526@yahoo.com.cn。
  • 作者简介:肖世金（1983—）, 男, 住院医师, 硕士；电子信箱: shjxiao@sina.com。

Expression of eosinophil chemotactic factor, regulated upon activation, normal T cell expressed and secreted and their receptors in maternal-fetal interface of spontaneous abortion

XIAO Shi-jin, DONG Qian, ZHAO Ai-min   

  1. Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2011-08-28 Published:2011-08-29

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摘要：

目的 探讨嗜酸性粒细胞趋化因子(Eotaxin)/CCR3、T淋巴细胞激活上调性表达分泌因子S(RANTES)/CCR5在正常妊娠免疫耐受及自然流产中的作用。方法 采集20例自然流产患者(AP组)和20例正常早孕妇女(NP组)的蜕膜和绒毛组织；采用免疫组织化学方法检测蜕膜和绒毛组织Eotaxin/CCR3、RANTES/CCR5的表达，并进行线性相关分析。结果 NP组蜕膜、绒毛组织Eotaxin的表达阳性率分别为75%和80%，显著高于AP组的35%和30%(P=0.011, P=0.001)；NP组阳性表达强度亦显著高于AP组(P=0.003, P=0.002)。NP组蜕膜、绒毛组织RANTES的表达阳性率分别为40%和45%，显著低于AP组的80%和85%(P=0.01, P=0.008)，NP组阳性表达强度亦显著低于AP组(P=0.002, P=0.002)。两组蜕膜、绒毛组织CCR5的表达阳性率比较，差异无统计学意义（P>0.05）；但AP组阳性表达强度显著高于NP组(P=0.018，P=0.029)。两组CCR3的表达阳性率及阳性表达强度比较，差异均无统计学意义（P>0.05）。在NP组中，蜕膜、绒毛RANTES的表达与CCR5的表达均呈正相关(r=0.679, P=0.001；r=0.478, P=0.033)；在AP组中，蜕膜、绒毛RANTES的表达与CCR5的表达均为正相关(r=0.610, P=0.004；r=0.620, P=0.004)；两组蜕膜、绒毛Eotaxin和CCR3的表达均不存在相关性 (P>0.05)。结论 趋化因子及其受体Eotaxin/CCR3、RANTES/CCR5在妊娠免疫耐受形成和自然流产的发病机制中可能发挥重要作用。

关键词: 趋化因子, 嗜酸性粒细胞趋化因子, CCR3, T淋巴细胞激活上调性表达分泌因子S, CCR5, 自然流产

Abstract:

Objective To investigate the role of eosinophil chemotactic factor (Eotaxin)/CCR3 and regulated upon activation, normal T cell expressed and secreted (RANTES)/CCR5 in immune tolerance of normal pregnancy and spontaneous abortion. Methods Decidua and chorionic tissues of 20 patients with spontaneous abortion (AP group) and 20 women with normal pregnancy (NP group) were collected. The expression of Eotaxin/CCR3 and RANTES/CCR5 in decidua and chorionic tissues was detected by immunohistochemistry, and linear correlation analysis was performed.ResultsThe positive expression rates of Eotaxin in decidua and chorionic tissues in NP group were significantly higher than those in AP group (75% vs 35%, P=0.011; 80% vs 30%, P=0.001), and the positive expression intensity in NP group was significantly higher than that in AP group (P=0.003, P=0.002). The positive expression rates of RANTES in decidua and chorionic tissues in NP group were significantly lower than those in AP group (40% vs 80%, P=0.01; 45% vs 85%, P=0.008), and the positive expression intensity in NP group was significantly lower than that in AP group (P=0.002, P=0.002). There was no significant difference in the positive expression rate of CCR5 in decidua and chorionic tissues between two groups (P>0.05), while the positive expression rates of AP group was significantly higher than those in NP group (P=0.018, P=0.029). There was no significant difference in the positive expression rate and positive expression intensity of CCR3 in decidua and chorionic tissues between two groups (P>0.05). In NP group, the expression of RANTES was positively related to the expression of CCR5 in decidua and chorionic tissues (r=0.679, P=0.001; r=0.478, P=0.033). In AP group, the expression of RANTES was positively related to the expression of CCR5 in decidua and chorionic tissues (r=0.610, P=0.004; r=0.620, P=0.004). There was no relationship between the expression of Eotaxin and expression of CCR3 in two groups (P>0.05). Conclusion Chemokines and their receptors Eotaxin/CCR3 and RANTES/CCR5 may play an important role in the pathogenesis of immune tolerance of pregnancy and spontaneous abortion.

Key words: chemokine, eosinophil chemotactic factor, CCR3, regulated upon activation, normal T cell expressed andsecreted, CCR5, spontaneous abortion