上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (11): 1564-.doi: 10.3969/j.issn.1674-8115.2011.11.013

• 论著（基础研究） • 上一篇    下一篇

葛根总黄酮联合三氧化二砷对Kasumi-1和HL-60细胞增殖和凋亡的影响

唐宇宏1, 邵化敏2, 朱红青3, 姜鹏君4, 季建敏4, 沈 群4   

  1. 1.上海交通大学 医学院附属第三人民医院血液科, 上海201900； 2.中国人民解放军第155医院血液肿瘤科, 开封 475003； 3.马鞍山市中心医院血液科, 马鞍山 243000； 4.南京中医药大学附属医院血液科, 南京 210009
  • 出版日期:2011-11-28 发布日期:2011-11-29
  • 通讯作者: 沈 群, 电子信箱: shenq@medmail.com.cn。
  • 作者简介:唐宇宏（1978—）, 男, 住院医师, 博士；电子信箱: yuhong_tang@126.com。
  • 基金资助:

    江苏省卫生厅“科教兴卫工程”医学重点人才基金（RC2007068）；江苏省“六大人才高峰”第五批高层次人才基金（2008年）

Effects of co-treatment with puerariae radix flavones and arsenic trioxide on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells

TANG Yu-hong1, SHAO Hua-min2, ZHU Hong-qing3, JIANG Peng-jun4, JI Jian-min4, SHEN Qun4   

  1. 1.Department of Hematology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China；2.Department of Hematology and Oncology, No.155 Hospital of People's Liberation Army, Kaifeng 475003, China；3.Department of Hematology, The Central Hospital of Maanshan, Maanshan 243000, China；4.Department of Hematology, the Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210009, China
  • Online:2011-11-28 Published:2011-11-29
  • Supported by:

    Foundation of Department of Health, Jiangsu Province, RC2007068；Talent Program of Jiangsu Province, 2008

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摘要：

目的 探讨葛根总黄酮（PRF）联合三氧化二砷（ATO）对M2型急性髓系白血病细胞株Kasumi-1和HL-60细胞增殖和凋亡的影响。方法 以100 μg/mL PRF和1 μmol/L ATO联合处理Kasumi-1和HL-60细胞48 h（RRF+ATO组），MTT法检测细胞增殖抑制率，Annexin V-FITC/PI双染法流式细胞术检测细胞早期凋亡率，Real-Time PCR检测凋亡抑制基因Bcl-2、Survivin mRNA表达，Western blotting检测细胞凋亡相关蛋白酶Caspase-3、-8、-9的表达。设立单用100 μg/mL PRF的PRF处理组和空白对照组。结果 RRF+ATO组Kasum-1细胞增殖抑制率、早期凋亡率以及Caspase-3、-9表达均显著高于PRF处理组（P<0.05），细胞中Bcl-2 mRNA表达的下调趋势明显。RRF+ATO组HL-60细胞各项指标检测结果与PRF处理组比较差异均无统计学意义(P>0.05)。结论 PRF联合ATO能有效抑制Kasumi-1细胞增殖并诱导早期凋亡，而对HL-60细胞则无明显影响。

关键词: 葛根总黄酮, 三氧化二砷, 细胞增殖, 细胞凋亡, M2型急性髓系白血病

Abstract:

Objective To investigate the effects of co-treatment with puerariae radix flavones (PRF) and arsenic trioxide (ATO) on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells of acute myeloid leukemia M2. Methods Kasumi-1 cells and HL-60 cells were treated with 100 μg/mL PRF and 1 μmol/L ATO for 48 h (RRF+ATO group). MTT assay was employed to measure the inhibition rate of cell proliferation, Annexin V-FITC/PI double staining was used to determine the early apoptosis rate by flow cytometry, the expression of Bcl-2 and Survivin mRNA was detected by Real-Time PCR, and the expression of Caspase-3, Caspase-8 and Caspase-9 protein was detected by Western blotting. Cells only treated with 100 μg/mL PRF were served as PRF group, and blank control group was also established. Results The inhibition rate of cell proliferation, early apoptosis rate and expression of Caspase-3 and Caspase-9 protein of Kasumi-1 cells in RRF+ATO group were significantly higher than those in PRF group (P<0.05), and there was an obvious trend of down-regulation of expression of Bcl-2 mRNA in Kasumi-1 cells. There was no significant difference in each parameter of HL-60 cells between RRF+ATO group and PRF group (P>0.05). Conclusion Co-treatment with PRF and ATO can effectively inhibit the proliferation and induce early apoptosis of Kasumi-1 cells, while that has no significant effect on HL-60 cells.

Key words: puerariae radix flavones, arsenic trioxide, proliferation, apoptosis, acute myeloid leukemia M2