上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (12): 1707-.doi: 10.3969/j.issn.1674-8115.2011.12.010

• 论著（基础研究） • 上一篇    下一篇

抗性淀粉干预对糖尿病大鼠子代糖代谢的影响

沈 丽1, 殷 峻2, 周 军3   

  1. 1.上海交通大学附属第六人民医院临床营养科, 上海 200233； 2.上海交通大学附属第六人民医院内分泌代谢科, 上海 200233； 3.美国华盛顿退伍军人医学中心内分泌代谢实验室, Washington DC 20422
  • 出版日期:2011-12-28 发布日期:2012-01-04
  • 作者简介:沈 丽（1975—）, 女, 主治医师, 博士；电子信箱: Shenli750@hotmail.com。

Effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats

SHEN Li1, YIN Jun2, ZHOU Jun3   

  1. 1.Department of Clinical Nutrition, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2.Department of Endocrinology and Metabolism, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；3.Laboratory of Endocrinology and Metabolism, Veterans Affairs Medical Center, Washington DC 20422, USA
  • Online:2011-12-28 Published:2012-01-04

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摘要：

目的 探讨抗性淀粉干预糖尿病大鼠对其子代糖代谢的影响。方法 20只6周龄雌性2型糖尿病Goto-Kakizaki（GK）大鼠随机分为抗性淀粉组（GK-RS组，n=10）和等能量密度饲料对照组（GK-EC组，n=10），以喂食等能量密度饲料的同龄Wistar雌鼠作为空白对照组（Wistar-EC组，n=10），分组喂养直至分娩。在各组幼鼠中分别随机选取10只雄性幼鼠，常规饲料喂养至8周龄，每周3次记录体质量和摄食量，免疫组织化学法检测胰腺相对β细胞密度，ELISA法测定空腹血清胰岛素水平，口服葡萄糖耐量试验检测空腹血糖和餐后2 h血糖，以稳态模型的胰岛素抵抗指数（HOMA-IR）评估胰岛素敏感性。结果 与GK-EC 组比较，GK-RS组空腹血糖浓度显著降低（P<0.05），胰腺胰岛素含量明显升高（P<0.05），胰腺相对β细胞密度呈现增高趋势（P=0.07）。GK-EC与GK-RS组幼鼠的摄食量、体质量、HOMA-IR、餐后2 h血糖和血清空腹胰岛素水平比较，差异均无统计学意义（P>0.05）。结论 抗性淀粉干预糖尿病大鼠有益于改善其子代发生的糖代谢紊乱。

关键词: 抗性淀粉, 2型糖尿病, 糖代谢, 子代

Abstract:

Objective To investigate the effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats. Methods Twenty female Goto-Kakizaki (GK) rats with type 2 diabetes aged 6 weeks were randomized into resistant starch diet group (GK-RS group, n=10) and energy control diet group (GK-EC group, n=10), and another 10 age-matched female Wistar rats fed with energy control diet were served as blank control group (Wistar-EC group). Ten male offsprings were randomly selected in each group, and were raised till 8 weeks old on regular chow diet. Body weight and volume of food intake were recorded three time a week, pancreatic relative β cell density was measured by immunohistochemistry, fasting serum insulin concentration was detected by ELISA, concentrations of fasting blood glucose and 2 h postprandial blood glucose were determined by oral glucose tolerance test, and insulin sensitivity was evaluated by homeostasis model assessment of insulin resistance (HOMA-IR). Results Compared with GK-EC group, the concentration of fasting blood glucose significantly decreased (P<0.05), pancreatic insulin content significantly increased (P<0.05), and pancreatic relative β cell density tended to increase in GK-RS group (P=0.07). There was no significant difference in body weight, volume of food intake, HOMA-IR and concentrations of 2 h postprandial blood glucose and fasting serum insulin between GK-EC group and GK-RS group (P>0.05). Conclusion Dietary resistant starch has potential in improving glucose metabolism disorder in offsprings born to diabetic dams.

Key words: resistant starch, type 2 diabetes, glucose metabolism, offspring