Abstract:

Objective To observe the effects of lipoic acid pretreatment on acute oxidative stress induced by intravenous iron supplement in patients with continuous ambulatory peritoneal dialysis (CAPD). Methods Forty patients with CAPD received single intravenous iron supplement (Fe group) and intravenous iron supplement with lipoic acid pretreatment (Lipoic+Fe group), and the intermission between these two treatment was more than 8 weeks. Blood samples were taken before (0 min) and 10, 60, 120 and 240 min after intravenous iron supplement, and the activity of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and level of serum malondiadehyde (MDA) were detected by visible range spectrophotometer. Another 20 healthy volunteers were served as control group. Results Compared with control group, the activity of SOD and GSH-Px in Fe group and Lipoic+Fe group at 0 min time point decreased, while the level of MDA increased （P<0.05）. After intravenous iron supplement in two groups, the activity of SOD and GSH-Px decreased and reached the valley at 60 min time point, and the level of MDA increased and reached the peak at 60 min time point. The activity of SOD and GSH-Px was significantly higher and the level of MDA was significantly lower at each time point in Lipoic+Fe group than those in Fe group (P<0.05). Conclusion Acute aggravation of oxidative stress may be induced by intravenous iron supplement in patients with CAPD, which may be alleviated by lipoic acid pretreatment.

Key words: oxidative stress, peritoneal dialysis, intravenous iron supplement, lipoic acid