Abstract: Objective · To investigate the effect of recombinant adiponectin on the pregnancy outcome and the balance of Th17/regulatory T cell (Treg)
in spontaneous abortion murine model. Methods · CBA/J(♀)×DBA/2(♂) mating was used as a spontaneous abortion model, while CBA/J(♀)×BALB/
c(♂) mating as a normal pregnancy model. CBA/J female mice were intraperitoneal injected with 10 μg/(kg·d) recombinant adiponectin for 9 d from day
5 of gestation. The control group received equal volumes of PBS each day. On day 14 of gestation, the mice were killed. The embryo resorption rate was
calculated. The levels of serum adiponectin, IL-17 and TGF-β were detected by ELISA. Spleen lymphocytes were isolated. The ratios of Th17/Treg were
counted by flow cytometry(FCM). Results · Recombinant adiponectin administration had no obvious effect on the embryo resorption rate of the normal
pregnancy model, while it significantly reduced the embryo resorption rate from 32.2% to 13.3% (P=0.039) of the spontaneous abortion model. ELISA
showed that recombinant adiponectin increased the serum adiponectin and TGF-β levels, and reduced the IL-17 level of the spontaneous abortion model
(P<0.01). FCM showed that adiponectin administraion could reduce the ratio of Th17/CD4+ T cells, increase the ratio of Treg/CD4+ T cells and reverse
the ratio of Th17/Treg in abortion mice. Conclusion · Intraperitoneal administration of recombinant adiponectin in early gestation can improve pregnancy
outcome in a murine model of abortion maybe through regulating the balance of Th17/Treg.

Key words: adiponectin, gestation, spontaneous abortion model, mouse, Th17, regulatory T cell