JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (7): 903-909.doi: 10.3969/j.issn.1674-8115.2021.07.009
• Clinical research • Previous Articles
Qing LIU1(), Na ZHANG1(
), Jing-bo SHAO1, Hong LI1, Kai CHEN1, Cheng-kan DU2, Zhen WANG1, Hui JIANG1
Online:
2021-07-28
Published:
2021-08-03
Contact:
Na ZHANG
E-mail:liuqing.ting@163.com;abczhangna@hotmail.com
Supported by:
CLC Number:
Qing LIU, Na ZHANG, Jing-bo SHAO, Hong LI, Kai CHEN, Cheng-kan DU, Zhen WANG, Hui JIANG. Clinical characteristics and prognosis of pediatric acute leukemia patients with MLL gene rearrangements[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(7): 903-909.
Method | ALL (n=27) | AML (n=18) |
---|---|---|
FISH | ||
MLL-r | 27 (100.0) | 18 (100.0) |
Karyotype analysis | ||
Normal Karyotype | 8 (29.6) | 2 (11.1) |
11q23 translocation | 13 (48.1) | 13 (72.2) |
Add (11q23) or del (11q23) | 2 (7.4) | 0 (0) |
Other aberration | 1 (3.7) | 1 (5.5) |
Not available | 3 (11.1) | 2 (11.1) |
RT-PCR | ||
MLL/AF4 | 16 (59.2) | 2 (11.1) |
MLL/AF9 | 2 (7.4) | 6 (33.3) |
MLL/AF10 | 4 (14.8) | 3 (16.7) |
MLL/ENL | 2 (7.4) | 3 (16.7) |
MLL/AF1q | 1 (3.7) | 1 (5.5) |
MLL/AF17 | 0 (0) | 1 (5.5) |
MLL-r negative | 2 (7.4) | 2 (11.1) |
Tab 1 Cytogenetic and molecular characteristics of 45 AL children with MLL-r [n(%)]
Method | ALL (n=27) | AML (n=18) |
---|---|---|
FISH | ||
MLL-r | 27 (100.0) | 18 (100.0) |
Karyotype analysis | ||
Normal Karyotype | 8 (29.6) | 2 (11.1) |
11q23 translocation | 13 (48.1) | 13 (72.2) |
Add (11q23) or del (11q23) | 2 (7.4) | 0 (0) |
Other aberration | 1 (3.7) | 1 (5.5) |
Not available | 3 (11.1) | 2 (11.1) |
RT-PCR | ||
MLL/AF4 | 16 (59.2) | 2 (11.1) |
MLL/AF9 | 2 (7.4) | 6 (33.3) |
MLL/AF10 | 4 (14.8) | 3 (16.7) |
MLL/ENL | 2 (7.4) | 3 (16.7) |
MLL/AF1q | 1 (3.7) | 1 (5.5) |
MLL/AF17 | 0 (0) | 1 (5.5) |
MLL-r negative | 2 (7.4) | 2 (11.1) |
Factor | ALL | AML | ||||||
---|---|---|---|---|---|---|---|---|
n | Median time/month | EFS/% | P value | n | Median time/month | EFS/% | P value | |
Gender | 0.998 | 0.539 | ||||||
Male | 13 | 20 | 40.0±16.9 | 8 | 25 | 41.7±22.2 | ||
Female | 11 | 23 | 46.8±16.7 | 10 | 16 | 41.5±20.7 | ||
Age | 0.028 | 0.449 | ||||||
>1 year | 17 | 26 | 49.6±15.1 | 16 | 14 | 35.0±18.0 | ||
≤1 year | 7 | 14 | 28.6±17.1 | 2 | 83 | 50.0±35.4 | ||
WBC/(×109·L-1) | 0.024 | 0.530 | ||||||
≥50 | 15 | 17 | 29.1±12.2 | 6 | 25 | 53.3±24.8 | ||
<50 | 9 | 32 | 75.0±21.7 | 12 | 16 | 24.6±19.8 | ||
Hb/(g·L-1) | 0.895 | 0.959 | ||||||
0?60 | 3 | 10 | 66.7±27.2 | 4 | 32 | 33.3±27.2 | ||
>60 | 21 | 23 | 42.5±12.4 | 14 | 16 | 38.4±19.4 | ||
PLT/(×109·L-1) | 0.027 | 0.535 | ||||||
≤30 | 7 | 10 | 19.0±16.8 | 4 | 12 | 0 | ||
>30 | 17 | 32 | 54.1±14.3 | 14 | 16 | 45.9±17.8 | ||
Partner gene | 0.223 | ? | ? | ? | 0.747 | |||
MLL/AF4 | 15 | 14 | 36.6±14.1 | |||||
MLL/AF9 | ? | ? | ? | 6 | 22.5 | 37.5±28.6 | ||
Others | 9 | 34 | 53.3±20.5 | 12 | 12 | 34.1±18.7 | ||
Blast count of PB | 0.214 | 0.872 | ||||||
Positive | 18 | 14 | 38.2±13.6 | 13 | 16 | 37.2±19.1 | ||
Negative | 6 | 58 | 60.0±21.9 | 5 | 22 | 37.5±28.6 | ||
Immunophenotyping | 0.547 | ? | ||||||
Pro-B | 16 | 23 | 40.5±13.7 | ? | ? | ? | ||
Others | 8 | 20 | 68.6±18.6 | ? | ? | ? |
Tab 2 Effects of different prognostic factors on the survival rates of MLL-r AL patients
Factor | ALL | AML | ||||||
---|---|---|---|---|---|---|---|---|
n | Median time/month | EFS/% | P value | n | Median time/month | EFS/% | P value | |
Gender | 0.998 | 0.539 | ||||||
Male | 13 | 20 | 40.0±16.9 | 8 | 25 | 41.7±22.2 | ||
Female | 11 | 23 | 46.8±16.7 | 10 | 16 | 41.5±20.7 | ||
Age | 0.028 | 0.449 | ||||||
>1 year | 17 | 26 | 49.6±15.1 | 16 | 14 | 35.0±18.0 | ||
≤1 year | 7 | 14 | 28.6±17.1 | 2 | 83 | 50.0±35.4 | ||
WBC/(×109·L-1) | 0.024 | 0.530 | ||||||
≥50 | 15 | 17 | 29.1±12.2 | 6 | 25 | 53.3±24.8 | ||
<50 | 9 | 32 | 75.0±21.7 | 12 | 16 | 24.6±19.8 | ||
Hb/(g·L-1) | 0.895 | 0.959 | ||||||
0?60 | 3 | 10 | 66.7±27.2 | 4 | 32 | 33.3±27.2 | ||
>60 | 21 | 23 | 42.5±12.4 | 14 | 16 | 38.4±19.4 | ||
PLT/(×109·L-1) | 0.027 | 0.535 | ||||||
≤30 | 7 | 10 | 19.0±16.8 | 4 | 12 | 0 | ||
>30 | 17 | 32 | 54.1±14.3 | 14 | 16 | 45.9±17.8 | ||
Partner gene | 0.223 | ? | ? | ? | 0.747 | |||
MLL/AF4 | 15 | 14 | 36.6±14.1 | |||||
MLL/AF9 | ? | ? | ? | 6 | 22.5 | 37.5±28.6 | ||
Others | 9 | 34 | 53.3±20.5 | 12 | 12 | 34.1±18.7 | ||
Blast count of PB | 0.214 | 0.872 | ||||||
Positive | 18 | 14 | 38.2±13.6 | 13 | 16 | 37.2±19.1 | ||
Negative | 6 | 58 | 60.0±21.9 | 5 | 22 | 37.5±28.6 | ||
Immunophenotyping | 0.547 | ? | ||||||
Pro-B | 16 | 23 | 40.5±13.7 | ? | ? | ? | ||
Others | 8 | 20 | 68.6±18.6 | ? | ? | ? |
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