Abstract:

Objective To investigate the fibrogenesis of dermal and adipose tissue-derived fibroblasts, and explore the mechanism. Methods Fibroblasts derived from dermal tissues and subcutaneous adipose tissues in the same pig were isolated and cultured. Inverted microscope and transmission electron microscope were used to observe the cell morphology and ultrastructure, and Real-Time PCR was employed to detect the relative expression of procollagen type ⅠmRNA, procollagen type Ⅲ mRNA, α-smooth muscle actin (α-SMA) mRNA, transforming growth factor β1 (TGF-β1) mRNA and insulin-like growth factor-1 (IGF-1) mRNA in cells. Results Compared with adipose tissue-derived fibroblasts, the cell body of dermal tissue-derived fibroblasts was larger, with expanded rough endoplasmic reticulum. The relative expression of precollagen type I mRNA and α-SMA mRNA in dermal tissue-derived fibroblasts was significantly higher than that in adipose tissue-derived fibroblasts (P<0.05), while the relative expression of precollagen type Ⅲ mRNA and IGF-1 mRNA in dermal tissue-derived fibroblasts was significantly lower than that in adipose tissue-derived fibroblasts (P<0.05). There was no significant difference in the relative expression of TGF-β1 mRNA between adipose tissue-derived fibroblasts and dermal tissuederived fibroblasts (P>0.05). Conclusion There are differences in cell morphology and function concerning with fibrogenesis between dermal tissuederived fibroblasts and adipose tissue-derived fibroblasts. The starting points of wound healing process after dermal and adipose tissue impairment are different between dermal tissue-derived fibroblasts and adipose tissue-derived fibroblasts.

Key words: dermal, adipose, fibroblast, collagen, fibrogenesis