Objective To investigate mycoplasma infection and its resistance to common antimicrobial agents and provide evidence for guiding clinical rational drug use. Methods A total of 2 639 samples from patients suspected of genitourinary tract infection were collected. Mycoplasma culture, identification, and drug sensitivity kit were used to perform mycoplasma detection, identification, and drug sensitivity tests. Results Three hundred and thirty-three of 2 639 samples were mycoplasma-positive and the positive rate was 12.6%. Two hundred and seventy-nine (83.8%) of these 333 samples were only Ureaplasma urealyticum (Uu)-positive, 23 (6.9%) were only Mycoplasma hominis (Mh)-positive, and 31 (9.3%) were Uu and Mh-positive. The detection rate in female patients (15.0%) was higher than that in male patients (4.3%). Mycoplasma-positive rate in 31-40-year-old female patients (23.6%) was higher than that in patients of other age groups. Antibiotic susceptibility rates of Uu to josamycin, minocycline, doxycycline, tetracycline, clarithromycin, ciprofloxacin, sparfloxacin, and erythromycin were 98.6%, 96.4%, 96.1%, 97.4%, 87.1%, 80.2%, 31.5%, and 26.2%, respectively. Conclusion Mycoplasma genitourinary tract infection is mainly caused by Uu. It is necessary to perform mycoplasma culture and drug sensitivity test for patients suspected of mycoplasma infection. Josamycin, doxycycline, and minocycline are recommended for empirical treatment.
