上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (3): 242-.doi: 10.3969/j.issn.1674-8115.2012.03.002

• 论著（基础研究） • 上一篇    下一篇

2型糖尿病大鼠胰岛组织中基质金属蛋白酶2的表达与活性研究

万晓玉, 董 艳, 林 宁, 张洪梅, 陈雪茹   

  1. 上海交通大学 医学院附属新华医院内分泌科, 上海 200092
  • 出版日期:2012-03-28 发布日期:2012-03-28
  • 通讯作者: 陈雪茹, 电子信箱: xueru1972@yahoo.com.cn。
  • 作者简介:万晓玉（1985—）, 女, 硕士生；电子信箱: wanxiaoyu1985@163.com。
  • 基金资助:

    国家自然科学基金（81170757）；上海交通大学医学院基金（09xj21011）

Expression and activity of matrix metalloproteinases 2 in pancreatic islets of rats with type 2 diabetes mellitus

WAN Xiao-yu, DONG Yan, LIN Ning, ZHANG Hong-mei, CHEN Xue-ru   

  1. Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2012-03-28 Published:2012-03-28
  • Supported by:

    National Natural Science Foundation of China, 81170757；Shanghai Jiaotong University School of Medicine Foundation, 09xj21011

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摘要：

目的 观察2型糖尿病大鼠胰岛组织中基质金属蛋白酶2 (MMP2)的表达及活性变化。方法 SD大鼠随机分为对照组和糖尿病组。利用高热量饮食喂养结合小剂量链脲佐菌素（STZ, 15 mg/kg）腹腔注射的方法建立2型糖尿病大鼠模型。分别于造模后2周和6周时提取大鼠胰岛组织，采用RT-PCR技术和Western blotting方法检测MMP2的mRNA和蛋白表达，以明胶酶谱分析MMP2活性。结果 两组大鼠胰岛组织中均存在MMP2的mRNA和蛋白表达，且糖尿病组显著高于对照组（P<0.05）；明胶酶谱分析结果显示：糖尿病组MMP2的酶活性显著高于对照组（P<0.05）。与造模后2周比较，糖尿病组造模后6周时MMP2的mRNA和蛋白表达及酶活性均较高，但差异无统计学意义（P>0.05）。结论 糖尿病大鼠胰岛组织中MMP2基因表达上调且酶活性增强,可能与2型糖尿病大鼠胰岛B细胞功能受损有关。

关键词: 2型糖尿病, 基质金属蛋白酶2, 酶活性, 胰岛

Abstract:

Objective To investigate the expression and activity of matrix metalloproteinases 2 (MMP2) in pancreatic islets of rats with type 2 diabetes mellitus. Methods SD rats were randomly divided into control group and diabetes mellitus group. Type 2 diabetes mellitus model was established by high calorie diet and intraperitoneal injection of low dose of streptozotocin (STZ, 15 mg/kg). The tissues of pancreatic islets were harvested from both groups 2 weeks and 6 weeks after model establishment, RT-PCR and Western blotting were employed to detect the expression of MMP2 mRNA and protein respectively, and the activity of MMP2 was analysed by gelatin zymography. Results There was expression of MMP2 mRNA and protein in tissues of pancreatic islets in both groups, and the expression of MMP2 mRNA and protein in diabetes mellitus group was significantly higher than that in control group (P<0.05). Gelatin zymography indicated that the activity of MMP2 in diabetes mellitus group was significantly higher than that in control group (P<0.05). The expression of MMP2 mRNA and protein and the activity of MMP2 6 weeks after model establishment were higher than those 2 weeks after model establishment in diabetes mellitus group, while there was no significant difference between them (P>0.05). Conclusion The expression of MMP2 gene and activity of MMP2 increase in pancreatic islets of rats with diabetes mellitus, which may be related to B cell dysfunction in rats with type 2 diabetes mellitus.

Key words: type 2 diabetes mellitus, matrix metalloproteinases 2, enzymatic activity, pancreatic islet